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Glycine type additive premixed feed for nursing sows

A technology for premixed feed and lactating sows is applied in the field of glycine-type additive premixed feed for lactating sows, which can solve the problem of no glycine, and achieve the effects of reducing pollution, reducing usage, and preventing the harm of drug-resistant bacteria.

Active Publication Date: 2018-03-06
福建九为生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, glycine is mainly used as a nutritional additive and attractant for increasing amino acids in feed for livestock and poultry, especially pets. So far, there are no reports of glycine promoting antibiotics to inhibit the growth of drug-resistant bact

Method used

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  • Glycine type additive premixed feed for nursing sows
  • Glycine type additive premixed feed for nursing sows
  • Glycine type additive premixed feed for nursing sows

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Glycine and glucose can increase the amount of antibiotics entering the bacterial body

[0028] Bacterial death was related to the amount of antibiotics that got inside the bacteria. In order to study the effects of glycine and glucose on promoting antibiotics to enter the bacteria, a single colony of Edwardsiella tarda EIB202 was picked from the LB plate and inoculated in 5 mL of LB medium, and cultured at 200 rpm at 30°C for 24 hours to reach saturation. Collect the bacterial liquid by centrifugation, centrifuge at 8000rpm for 5min, remove the supernatant and wash the bacterial cells with 0.85% normal saline, and finally suspend the bacterial cells with 1×M9 (containing 10mM acetate), adjust the OD value of the bacterial solution to 0.2, and then pack them separately 5mL in a test tube for later use. The experiment was divided into 5 groups, of which 2 groups were control groups, without adding any substances and adding antibiotics; the other 3 groups were...

Embodiment 2

[0029] Embodiment 2: Glycine and glucose can improve the susceptibility of various bacteria to kanamycin antibiotic

[0030]Pick a variety of bacteria: Staphylococcus aureus (S.aureus), Pseudomonas aeruginosa (Pseudomonas aeruginosa, P.aeruginosa), Escherichia coli clinical drug-resistant bacteria (Y15), Vibrio alginolyticus (V.alginolyticus) Put the single clone into 100ml LB liquid medium, culture at 200rpm at 37°C or 30°C for 16h to reach saturation. Collect 20ml of bacterial liquid, centrifuge at 8000rpm for 5min, remove the supernatant and wash the bacterial cells with an equal volume of 0.85% normal saline, and finally suspend the bacterial cells with 1×M9 (containing 10mM acetate), adjust the OD of the bacterial solution to 0.2, and divide Put 5mL in a test tube, add kanamycin as the control group, add 20mM glycine, 10mM glucose, 20mM glycine and 10mM glucose respectively as the experimental group, incubate at 37°C or 30°C at 200rpm for 6h, take 100μL of bacteria liqui...

Embodiment 3

[0031] Embodiment 3: Glycine and / or glucose improve the sensitivity of Escherichia coli and its clinical drug-resistant bacteria to oxytetracycline

[0032] (1) Glycine and / or glucose increase the sensitivity of Escherichia coli to oxytetracycline

[0033] Preparation of Escherichia coli test samples: Pick a single colony of Escherichia coli from the LB plate and inoculate it in 5mL of LB medium, shake and culture at 200rpm at 37°C for 16h to reach saturation. Collect the bacterial liquid by centrifugation, centrifuge at 8000rpm for 5min, remove the supernatant and wash the bacterial cells with 0.85% normal saline, and finally suspend the bacterial cells with 1×M9 (containing 10mM acetate), adjust the OD value of the bacterial solution to 0.2, and then pack them separately 5mL in a test tube for later use.

[0034] The prepared samples were divided into 5 groups, of which 2 groups were control groups, which were without adding any substances and adding oxytetracycline; the ot...

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Abstract

The invention discloses glycine type additive premixed feed for nursing sows and relates to mixed feed. The glycine type additive premixed feed for nursing sows is composed of the raw materials: glycine, glucose, exopolysaccharides, a copper glycine preparation, an iron glycine preparation, a zinc glycine preparation, a manganese glycine preparation, an organic iodine selenium and cobalt preformulation, calcium dihydrogen phosphate, calcium hydrogen phosphate, organic calcium, stone powder, table salt, betaine, choline, multiple vitamins, lysine, methionine, threonine, tryptophan, a sweeteningagent, an antioxidant, a mildew inhibitor, an enzyme preparation, phytase, an acidifier, Chinese herbal medicines and a carrier. By addition of the glycine and/or glucose, the survival rate of clinical drug-resistant bacteria of escherichia coli treated by terramycin obviously declines, which shows that the two substances, namely glycine and glucose can improve the sensibility of the clinical drug-resistant bacteria of escherichia coli on terramycin and also have a synergistic effect.

Description

technical field [0001] The invention relates to a mixed feed, in particular to a glycine type additive premixed feed for lactating sows. Background technique [0002] Antibiotics are used as feed drug additives in existing conventional additive premixed feeds for lactating sows, and inorganic copper sulfate pentahydrate, ferrous sulfate monohydrate, zinc sulfate monohydrate, manganese sulfate monohydrate and other metal trace element feed additives are used at the same time. And inorganic phosphorus, long-term feeding of sows with additive premixed feed for conventional lactating sows may cause antibiotic resistance in offspring piglets and environmental pollution caused by excessive undigested and absorbed antibiotics with manure. In addition, copper, iron, zinc, manganese and other inorganic metal trace element feed additives such as copper, iron, zinc and manganese and inorganic phosphorus are widely used in the feed, and the digestion and absorption rate of them by the a...

Claims

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Application Information

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IPC IPC(8): A23K50/30A23K20/163A23K20/142
CPCA23K20/142A23K20/163A23K50/30
Inventor 谢荔朋
Owner 福建九为生物技术有限公司