Affinity Chromatography Medium Using Tetrapeptide Gastrin as Functional Ligand
A technology of tetrapeptide gastrin and chromatography medium, which is applied in the field of protein chromatography separation technology, can solve the problems of high separation cost and difficult separation cost, and achieves remarkable hydrophobic charge-induced chromatography separation characteristics and adsorption capacity. Large, high ligand density effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0023] Get polychitosan 10g, add 25% (v / v) dimethyl sulfoxide of 2g, the sodium hydroxide of 5g divinyl sulfoxide and 3g, activate 10 hours in 200rpm shaking table under 25 ℃, suction filtration , washed with deionized water to obtain an activated chromatography matrix; the activated chromatography matrix was mixed with 10 g of 3-mercaptopropionic acid and 2 g of ammonium persulfate for alcoholization, reacted in a shaking table at 200 rpm at 50 ° C for 3 hours, filtered with suction, and used to remove Wash with ionized water to obtain an alcoholized matrix; mix the alcoholized matrix with 1 g of tetrapeptide gastrin and 0.5M sodium carbonate buffer, the pH of the sodium carbonate buffer is 10, and react in a shaker at 200 rpm at 30°C for 10 hours. The medium after tetrapeptide gastrin coupling was obtained; the medium after tetrapeptide gastrin coupling was suction-filtered, washed with deionized water, added to cyclohexylamine containing 50 g, and reacted in a shaker at 200 ...
Embodiment 2
[0025] Get 10g of polychitosan, add 5g of 25% (v / v) dimethyl sulfoxide, 10g of divinyl sulfoxide and 5g of sodium hydroxide, activate it in a shaker at 200rpm at 25°C for 12 hours, and filter with suction , washed with deionized water to obtain an activated chromatography matrix; the activated chromatography matrix was mixed with 10 g of 3-mercaptopropionic acid and 2 g of ammonium persulfate for alcoholization, reacted in a shaking table at 200 rpm at 50 ° C for 3 hours, filtered with suction, and used to remove Wash with ionized water to obtain alcoholized matrix; mix the alcoholized matrix with 1 g of tetrapeptide gastrin and 0.5M sodium carbonate buffer, the pH of the sodium carbonate buffer is 10, react in a shaker at 200 rpm at 30°C for 8 hours, Obtain the tetrapeptide gastrin-coupled medium; suction filter the tetrapeptide gastrin-coupled medium, wash with deionized water, add to cyclohexylamine containing 10 g, and react in a shaker at 30°C and 200rpm for 7 hours, wash...
Embodiment 3
[0027] Get polychitosan 10g, add 25% (v / v) dimethyl sulfoxide of 2g, the sodium hydroxide of 10g divinyl sulfoxide and the sodium hydroxide of 5g, activate 10 hours in 200rpm shaking table under 25 ℃, suction filtration , washed with deionized water to obtain an activated chromatography matrix; the activated chromatography matrix was mixed with 10 g of 3-mercaptopropionic acid and 3 g of ammonium persulfate for alcoholization, reacted in a shaker at 200 rpm at 50 ° C for 6 hours, filtered with suction, and used to remove Wash with ionized water to obtain an alcoholized matrix; mix the alcoholized matrix with 2g of tetrapeptide gastrin and 0.5M sodium carbonate buffer, the pH of the sodium carbonate buffer is 10, and react in a shaker at 200 rpm at 30°C for 20 hours. The tetrapeptide gastrin-coupled medium was obtained; the tetrapeptide gastrin-coupled medium was suction-filtered, washed with deionized water, added to cyclohexylamine containing 10 g, and reacted in a shaker at 2...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More