Urine preservation reagent and application thereof

A technology for preserving reagents and urine, which is applied in the field of urine preservation reagents, can solve the problems of low free DNA content, free DNA not reaching the detection level, and affecting the accuracy of test results, so as to avoid nuclease degradation and reduce genome size. DNA background, the effect of avoiding self-degradation

Inactive Publication Date: 2018-04-06
GENETALKS BIO TECH CHANGSHA CO LTD
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  • Abstract
  • Description
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Problems solved by technology

[0004] However, the free DNA content in urine is very low, and it is easily degraded in the urine environment. At the same time, the leukocyte fragmentation in urine will release a large amount of genomic DNA
This makes, on the one hand, the content o

Method used

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  • Urine preservation reagent and application thereof
  • Urine preservation reagent and application thereof
  • Urine preservation reagent and application thereof

Examples

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Embodiment

[0032] The urine preservation reagent in this example is composed of nucleic acid buffer, metal ion chelating agent, preservative, cell fixative and formaldehyde quencher, wherein the nucleic acid buffer is Tris-HCl with a final concentration of 2 mmol / L, and the working pH is 8 ; Metal ion chelating agent is the EDTA of final concentration 10mmol / L; Preservative is made up of the sodium citrate of the citric acid of final concentration 1.25mmol / L and final concentration 3.75mmol / L; Cell fixative is made up of equal amounts of paraformaldehyde, Composed of diazolidinyl urea and imidazolidinyl urea, the amount of cell fixative is 0.1% of the total weight; the formaldehyde quencher is composed of equal amounts of glycine, lysine and ethylenediamine, and the mixed total formaldehyde quencher The final concentration is 40mmol / L.

[0033] The preparation method of the urine preservation reagent is as follows:

[0034]Dissolve Tris in water, adjust the pH to 7-8 with hydrochloric a...

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Abstract

The invention discloses a urine preservation reagent and an application thereof. The urine preservation reagent comprises a nucleic acid buffer, a metal ion chelating agent, an antiseptic, a cell fixing agent, and a formaldehyde quenching agent; a nucleic acid buffer is Tris-HCl, a working pH value is 7-8, the metal ion chelating agent is EDTA, the antiseptic is citric acid and/or sodium citrate,the cell fixing agent is selected from at least one of paraformaldehyde, diazolidinyl urea, imidazolidinyl urea, and diethyl urea; and the formaldehyde quenching agent is selected from at least one ofglycine, lysine, ethene diamine and arginine. The urine preservation reagent can effectively preserve free DNA in urine, degradation can be avoided, and the urine preservation reagent establishes a base for subsequently detecting the free DNA; fragmentation of cells in the urine is prevented, a genome DNA background is reduced, and free DNA detection result accuracy is guaranteed. The urine preservation reagent is the novel reagent for preserving a urine sample, and provides the guarantee for clinical detection of the urine free DNA.

Description

technical field [0001] The present application relates to the field of urine preservation, in particular to a urine preservation reagent and its application. Background technique [0002] Urine is excreted through a series of tissues (such as the bladder), in which there are exfoliated cells and free DNA of related tissues. Urine is a complex metabolic waste excreted by the human body, including various organic and inorganic metabolites; many pathological metabolites also include proteins, carbohydrates, etc.; some patients have extremely serious hematuria. At the same time, the complexity of urine components allows many microorganisms to multiply rapidly in it, and the characteristics of urine itself make the nucleic acids in it easily degraded. [0003] Clinically, lesion tissues and cells are mainly used to detect and monitor tumor patients. The disadvantages lie in the heterogeneity of tissues, the trauma to patients, and the difficulty of sampling. With the developmen...

Claims

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Application Information

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IPC IPC(8): C12Q1/6806
CPCC12Q1/6806C12Q2527/125
Inventor 宋卓王永利高堂杰何庆
Owner GENETALKS BIO TECH CHANGSHA CO LTD
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