Liposome loaded with sea cucumber boiled liquid extract and preparation method thereof

Abstract

The invention relates to the technical field of medicines and cosmetics, and discloses a liposome carrying a sea cucumber boiled liquid extract and a preparation method thereof. The liposome carrier carries the sea cucumber boiled liquid extract that has biological activities like fungal resistance, anti-inflammation, virus resistance and radiation resistance and multiple skincare functions of resisting aging and oxidation and whitening. The liposome is prepared from a sea cucumber boiled liquid extract, a phospholipid material, cholesterol and an emulsifier through a thin-film dispersion combined freeze-thaw method. The sea cucumber boiled liquid extract is prepared into liposome, so that defects of the existing sea cucumber boiled liquid extract in preparation of special medicines and functional cosmetics are overcome, the sea cucumber boiled liquid extract is more stable, and the color and odor of the sea cucumber boiled liquid extract are removed. The preparation method is easy andcontrollable, has high repeatability, and facilitates industrial production.

Description

technical field

[0001] The invention relates to the technical fields of medicine and cosmetics, in particular to a liposome of sea cucumber boiled liquid extract and a preparation method thereof. Background technique

[0002] Sea cucumber boiling liquid is a large amount of waste liquid rich in active substances such as sea cucumber saponins, sea cucumber polypeptides and sea cucumber polysaccharides produced during sea cucumber processing. It used to be discharged as waste liquid, which would cause waste of resources and environmental pollution.

[0003] In recent years, the potential economic value of sea cucumber boiled liquid has attracted people's attention, and its application in medicine, cosmetics, food and other fields has been comprehensively developed. Chinese patent 201510020329.9 discloses a method for preparing oral liquid from sea cucumber boiled liquid, which uses discarded sea cucumber boiled liquid as raw material, removes heavy metals and impurities, conce...

Examples

Embodiment 1

[0039] The formula of raw material component is as follows:

[0040]

[0041] like figure 1 Shown, the preparation concrete steps of this embodiment are as follows:

[0042] 1. Weigh soybean lecithin, cholesterol and Span 80 according to the proportion of the formula, mix the raw materials and dissolve them in ether at 30°C, remove the ether by rotary evaporation until a uniform lipid film is formed at the bottom of the evaporation container;

[0043] 2. Weigh the sea cucumber boiled liquid extract and deionized water according to the formula ratio, mix the raw materials, place them in a constant temperature stirrer with a stirring rate of 500r / min, and stir for 20 minutes at 30°C to obtain a clear and transparent sea cucumber boiled liquid extract aqueous solution;

[0044] 3. Weigh Tween 80 and PBS buffer solution (pH value = 7.6) according to the formula ratio, add Tween 80, 1 / 2 PBS buffer solution and the aqueous solution of sea cucumber boiled liquid extract obtained...

Embodiment 2

[0047] The formula of raw material component is as follows:

[0048]

[0049] Such as figure 1 Shown, the preparation concrete steps of this embodiment are as follows:

[0050] 1. Weigh soybean lecithin, cholesterol and Span 80 according to the proportion of the formula, mix the raw materials and dissolve them in ether at 40°C, remove the ether by rotary evaporation until a uniform lipid film is formed at the bottom of the evaporation container;

[0051] 2. Weigh the sea cucumber boiled liquid extract and deionized water according to the formula ratio, mix the raw materials, place them in a constant temperature stirrer with a stirring rate of 300r / min and stir for 20min at 30°C to obtain a clear and transparent sea cucumber boiled liquid extract aqueous solution;

[0052] 3. Weigh Tween 80 and PBS buffer solution (pH value = 6.5) according to the formula ratio, add Tween 80, 1 / 2 PBS buffer solution and the aqueous solution of sea cucumber boiled liquid extract obtained in...

Embodiment 3

[0055] The formula of composition raw material component is as follows:

[0056]

[0057]

[0058] Such as figure 1 Shown, the preparation concrete steps of this embodiment are as follows:

[0059] 1. Weigh soybean lecithin, cholesterol and Span 80 according to the proportion of the formula, mix the raw materials and dissolve them in ether at 40°C, remove the ether by rotary evaporation until a uniform lipid film is formed at the bottom of the evaporation container;

[0060] 2. Weigh the sea cucumber boiled liquid extract and deionized water according to the formula ratio, mix the raw materials, place them in a constant temperature stirrer with a stirring rate of 600r / min, and stir for 15 minutes at 50°C to obtain a clear and transparent sea cucumber boiled liquid extract aqueous solution;

[0061] 3. Weigh Tween 80 and PBS buffer solution (pH value = 6.5) according to the formula ratio, add Tween 80, 1 / 3 PBS buffer solution and the aqueous solution of sea cucumber boi...

technical field

[0001] The invention relates to the technical fields of medicine and cosmetics, in particular to a liposome of sea cucumber boiled liquid extract and a preparation method thereof. Background technique

[0002] Sea cucumber boiling liquid is a large amount of waste liquid rich in active substances such as sea cucumber saponins, sea cucumber polypeptides and sea cucumber polysaccharides produced during sea cucumber processing. It used to be discharged as waste liquid, which would cause waste of resources and environmental pollution.

[0003] In recent years, the potential economic value of sea cucumber boiled liquid has attracted people's attention, and its application in medicine, cosmetics, food and other fields has been comprehensively developed. Chinese patent 201510020329.9 discloses a method for preparing oral liquid from sea cucumber boiled liquid, which uses discarded sea cucumber boiled liquid as raw material, removes heavy metals and impurities, conce...

Examples

Embodiment 1

[0039] The formula of raw material component is as follows:

[0040]

[0041] like figure 1 Shown, the preparation concrete steps of this embodiment are as follows:

[0042] 1. Weigh soybean lecithin, cholesterol and Span 80 according to the proportion of the formula, mix the raw materials and dissolve them in ether at 30°C, remove the ether by rotary evaporation until a uniform lipid film is formed at the bottom of the evaporation container;

[0043] 2. Weigh the sea cucumber boiled liquid extract and deionized water according to the formula ratio, mix the raw materials, place them in a constant temperature stirrer with a stirring rate of 500r / min, and stir for 20 minutes at 30°C to obtain a clear and transparent sea cucumber boiled liquid extract aqueous solution;

[0044] 3. Weigh Tween 80 and PBS buffer solution (pH value = 7.6) according to the formula ratio, add Tween 80, 1 / 2 PBS buffer solution and the aqueous solution of sea cucumber boiled liquid extract obtained...

Embodiment 2

[0047] The formula of raw material component is as follows:

[0048]

[0049] Such as figure 1 Shown, the preparation concrete steps of this embodiment are as follows:

[0050] 1. Weigh soybean lecithin, cholesterol and Span 80 according to the proportion of the formula, mix the raw materials and dissolve them in ether at 40°C, remove the ether by rotary evaporation until a uniform lipid film is formed at the bottom of the evaporation container;

[0051] 2. Weigh the sea cucumber boiled liquid extract and deionized water according to the formula ratio, mix the raw materials, place them in a constant temperature stirrer with a stirring rate of 300r / min and stir for 20min at 30°C to obtain a clear and transparent sea cucumber boiled liquid extract aqueous solution;

[0052] 3. Weigh Tween 80 and PBS buffer solution (pH value = 6.5) according to the formula ratio, add Tween 80, 1 / 2 PBS buffer solution and the aqueous solution of sea cucumber boiled liquid extract obtained in...

Embodiment 3

[0055] The formula of composition raw material component is as follows:

[0056]

[0057]

[0058] Such as figure 1 Shown, the preparation concrete steps of this embodiment are as follows:

[0059] 1. Weigh soybean lecithin, cholesterol and Span 80 according to the proportion of the formula, mix the raw materials and dissolve them in ether at 40°C, remove the ether by rotary evaporation until a uniform lipid film is formed at the bottom of the evaporation container;

[0060] 2. Weigh the sea cucumber boiled liquid extract and deionized water according to the formula ratio, mix the raw materials, place them in a constant temperature stirrer with a stirring rate of 600r / min, and stir for 15 minutes at 50°C to obtain a clear and transparent sea cucumber boiled liquid extract aqueous solution;

[0061] 3. Weigh Tween 80 and PBS buffer solution (pH value = 6.5) according to the formula ratio, add Tween 80, 1 / 3 PBS buffer solution and the aqueous solution of sea cucumber boi...

Images