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A kind of detection method of lincomycin residue in soil

A lincomycin and detection method technology, which is applied in measurement devices, instruments, scientific instruments and other directions, can solve problems such as accurate detection, and achieve the effect of improving accuracy and high sensitivity

Active Publication Date: 2021-02-23
NINGXIA HOPE FIELD BIOLOGICAL AGRI TECH CO LTD
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Problems solved by technology

[0004] The purpose of the present invention is to make up for the defects in the prior art that cannot quickly / accurately detect lincomycin in soil, there is no effective solution to the problem of matrix effect, and to provide a method for extracting lincomycin in soil and determining the residual amount method, in order to provide a method basis for the detection of lincomycin residues in soil

Method used

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  • A kind of detection method of lincomycin residue in soil
  • A kind of detection method of lincomycin residue in soil
  • A kind of detection method of lincomycin residue in soil

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specific Embodiment approach 1

[0023] Specific embodiment one: the detection method of lincomycin residue in a kind of soil of the present embodiment, it is carried out according to the following steps:

[0024] 1. Sample preparation for the test product:

[0025] 1) Take the soil containing lincomycin, add extractant, vortex for 1 min, assisted extraction in ultrasound for 30 min, centrifuge at 3000-4000 rpm for 10 min, and take the supernatant;

[0026] 2) Evaporate the supernatant in a 30°C water bath to nearly dryness, and then redissolve it in ultrapure water to obtain the sample to be treated;

[0027] 3) Pipette methanol and ultrapure water to activate the HLB solid-phase extraction column, add the sample to be treated, pass through the column at a speed of 1mL / min, and rinse with ultrapure water after all the sample liquid flows out. After vacuuming for 5-10 minutes, the analyte was eluted with 80% methanol by volume, the eluate was collected and fixed to volume, shaken evenly, and passed through a...

specific Embodiment approach 2

[0032] Embodiment 2: This embodiment differs from Embodiment 1 in that the mass-volume ratio of lincomycin soil to extractant is 1 g: 10 mL. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0033] Embodiment 3: This embodiment differs from Embodiment 1 in that: the extractant is mixed with phosphate and acetonitrile in a ratio of 3:4 by volume. Others are the same as in the first embodiment.

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Abstract

A detection method for lincomycin residue in soil, which relates to a detection method for lincomycin residue titer in soil, the method comprises the preparation of reference substance solution, the preparation of test solution, and then adopts high-efficiency For detection by liquid chromatography, the above-mentioned standard solution and the test solution are injected into the liquid chromatograph, and the lincomycin titer is calculated according to the external standard method. The present invention establishes a high-performance liquid chromatography detection and analysis method for lincomycin in soil by optimizing the chromatographic conditions and testing the preparation methods of the test product and standard product; by optimizing the chromatographic conditions and purification process, the soil In the detection method of lincomycin, through the methodological verification test research, it is shown that the established method has high accuracy, strong specificity and good reproducibility, and can effectively detect residual lincomycin in soil.

Description

technical field [0001] The invention relates to the technical field of soil detection and testing, in particular to a detection method for lincomycin residual potency in soil. Background technique [0002] Veterinary antibiotics are used to prevent and treat animal diseases, improve feed utilization and promote animal growth. With the expansion of the scale of poultry farming, the use of antibiotics has also increased. Veterinary antibiotics are mainly excreted into the soil in the form of original drugs and metabolites in the form of feces and urine after passing through the animal body, which leads to the pollution of antibiotics in the soil and poses a threat to ecological security and human health. Relevant tests have found that the content of antibiotics in the soil has reached 11-300 μg / kg, mainly sulfonamides, quinolones, and tetracyclines. The establishment of trace detection and analysis methods for different types of antibiotics in soil has become a relatively po...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 王义张鹏金昌福李宁飞
Owner NINGXIA HOPE FIELD BIOLOGICAL AGRI TECH CO LTD
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