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Implant and method for producing an implant

A technology of implants and solutions, applied in the field of implants, can solve problems such as non-retention, and achieve the effect of improving tissue processing

Inactive Publication Date: 2018-04-27
威德瑞真有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, SIS does not preserve the original vascular supply to the tissue, resulting in limited perfusion

Method used

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  • Implant and method for producing an implant
  • Implant and method for producing an implant
  • Implant and method for producing an implant

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Experimental program
Comparison scheme
Effect test

Embodiment

[0090] All reagents were purchased from Sigma-Aldrich (UK) or Fisher Scientific (UK).

[0091] All animals were maintained and handled in accordance with the Animals Scientific Procedures Act 1986 (Animals Scientific Procedures Act 1986), and research was carried out through the following guidelines prescribed and licensed by the UK Home Office. Experiments were carried out using Large White Landrace crossbreed pigs. All animals were maintained under standard laboratory conditions and fed commercial pelleted chow.

[0092] 1. Preparation of decellularized scaffolds

[0093] (a) Harvested porcine intestinal tissue

[0094] After intravenous injection of heparin (7 000 U) in 60-65 kg English Large White Landrace crossbred pigs, a midline incision was made and the ileal part was separated from its connected vascular supply approximately 20-30 cm long. Dissect the remaining lymph nodes of the mesentery; keep the entire sample leak-proof; tie and separate the distal end of t...

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PUM

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Abstract

The invention relates to an implant and a method for producing the implant. According to the invention, intestinal tissue comprising a tubular segment of intestine with at least part of its associatedvasculature intact is processed by: perfusing the vasculature through a vessel thereof with at least one decellularising medium; and separately perfusing the tubular segment of intestine through itslumen with at least one decellularising medium. The new method greatly improves tissue processing as compared with the methods previously known in the art, in which decellularisation solutions were perfused into the tissue via the vasculature only, relying on diffusion to decellularise and purify the small bowel tissue. In contrast, the invention provides for separate decellularisation and purification protocols for the vascular and tubular intestinal components, which are designed to optimize decellularisation and purification of each of these tissue structures while preserving certain tissue-specific three-dimensional structures. The resulting scaffold is substantially decellularised and provides an excellent implant for repair and regeneration of bowel tissue.

Description

[0001] This application is a divisional application of Chinese Patent Application No. 201380048678.0 with the filing date of July 16, 2013, and the title of the invention is "Implants and methods for producing implants". technical field [0002] The present invention relates to implants and methods for producing implants. The invention is particularly useful in the production of intestinal-derived implants. Background technique [0003] Implants comprising biologically derived scaffolds have become an important option for tissue / organ repair and regeneration in the treatment of various diseases and conditions. A continuing major obstacle is the requirement for a functional blood supply of the implanted stent. [0004] Patients with Short Bowel Syndrome (SBS) lack more than half of their small intestine, which can result from numerous disorders and often results in surgical resection. A suitable implant would be useful in the treatment of SBS. Intestinal diseases such as C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
CPCA61L27/3629A61L27/3691A61P1/04A61P41/00A61B17/1114A61F2/062A61F2/064A61L27/3604A61F2/04A61F2002/045
Inventor 保罗·大卫·西本斯塔赫拉·伊克巴尔·安萨里
Owner 威德瑞真有限公司