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Molybdenum disulfide used for stem cell proliferation and/or differentiation, substrate for stem cell proliferation and/or differentiation, preparation method and application

A molybdenum disulfide, neural stem cell technology, applied in biochemical equipment and methods, cell culture supports/coatings, animal cells, etc., can solve the problem of graphene's poor biocompatibility, lack of regulation of stem cell proliferation and differentiation, and electrical conductivity. Sexual irreducibility, etc.

Active Publication Date: 2020-11-03
BEIJING INST OF NANOENERGY & NANOSYST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, graphene has poor biocompatibility with stem cells, its conductivity cannot be adjusted, and it is still lacking in the regulation of proliferation and differentiation of stem cells.

Method used

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  • Molybdenum disulfide used for stem cell proliferation and/or differentiation, substrate for stem cell proliferation and/or differentiation, preparation method and application
  • Molybdenum disulfide used for stem cell proliferation and/or differentiation, substrate for stem cell proliferation and/or differentiation, preparation method and application
  • Molybdenum disulfide used for stem cell proliferation and/or differentiation, substrate for stem cell proliferation and/or differentiation, preparation method and application

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preparation example Construction

[0038] The preparation method of the molybdenum disulfide thin film, the characteristics of the molybdenum disulfide thin film and the selection of stem cells have been specifically introduced above, and the present invention will not repeat them here.

[0039] According to the present invention, the substrate used for the above molybdenum disulfide thin film can be various substrates known in the art for cell culture, for example, a glass substrate or a polymer film substrate. The polymer membrane can be selected in a wide range, as long as the selected membrane does not decompose at a temperature not higher than 180°C, has good biocompatibility with cells and is non-toxic to cells. Just poison. Examples of the polymer film include, but are not limited to, polyvinylidene fluoride films.

[0040]The inventors of the present invention have found that when the substrate is a flexible polymer film substrate for the cultivation of stem cells, after the cells are inoculated and th...

Embodiment 1

[0053] This embodiment is used to illustrate the substrate for stem cell proliferation and / or differentiation provided by the present invention

[0054] (1) First weigh 30 mg of sodium molybdate powder and 60 mg of thiourea powder, and dissolve them in 20 ml of deionized water. After the two powders are fully dissolved, pour the mixture into a 25ml Teflon reaction kettle. Cut the glass into 20mm x 30mm size, soak it in the reaction kettle, and react at 180°C for 24 hours.

[0055] (2) The glass synthesized in step (1) with attached molybdenum disulfide was ultrasonically treated at a frequency of 55 Hz and a temperature of 25° C. for 10 min. After treatment, the molybdenum disulfide film layer can be firmly attached to the glass surface as a substrate for cell culture.

[0056] The thickness of the molybdenum disulfide thin film is 300nm. After the substrate was characterized by scanning electron microscopy, it can be seen that the hydrothermally synthesized molybdenum disu...

Embodiment 2

[0058] This embodiment is used to illustrate the substrate for stem cell proliferation and / or differentiation provided by the present invention

[0059] (1) First weigh 25mg of sodium molybdate powder and 50mg of thiourea powder, and dissolve them in 20ml of deionized water. After the two powders are fully dissolved, pour the mixture into a 25ml Teflon reaction kettle. Cut the polyvinylidene fluoride film into a size of 20mm x 30mm, soak it in a reaction kettle, and react at 200°C for 24 hours.

[0060] (2) The polyvinylidene fluoride film attached with molybdenum disulfide synthesized in step (1) was ultrasonically treated at a frequency of 50 Hz and a temperature of 28° C. for 10 min. After treatment, the thin layer of molybdenum disulfide can be firmly attached to the surface of polyvinylidene fluoride membrane as a substrate for cell culture. The substrate structure is as follows: Figure 12 shown.

[0061] The thickness of the molybdenum disulfide thin film is 500nm. A...

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Abstract

The invention relates to the field of biomaterials and discloses a use of molybdenum disulfide for stem cell proliferation and / or differentiation, a substrate for stem cell proliferation and / or differentiation and a preparation method and use thereof and discloses a use of molybdenum disulfide in stem cell proliferation and / or differentiation, a substrate for stem cell proliferation and / or differentiation and a preparation method thereof. The substrate comprises a base and a molybdenum disulfide film coating the base. The invention discloses a use of the substrate in stem cell proliferation and / or differentiation. When the molybdenum disulfide film is used in stem cell proliferation and / or differentiation, stem cell activity and dryness are not reduced, good biocompatibility to stem cellsis obtained and cell proliferation and differentiation are promoted. Molybdenum disulfide as a biomaterial has a wide application prospect in stem cell proliferation and / or differentiation.

Description

technical field [0001] The present invention relates to the field of biomaterials. Specifically, it relates to the application of molybdenum disulfide in stem cell proliferation and / or differentiation, a substrate for stem cell proliferation and / or differentiation, the preparation method of the substrate and the effect of the substrate in stem cell proliferation and / or differentiation application. Background technique [0002] To realize the directed and controllable differentiation of stem cells has always been the core and main goal in the field of tissue engineering and regenerative medicine. Many studies have shown that biomaterial-mediated physical (such as material hardness, surface topography, and surface charge) and chemical factors (chemical composition) can affect the fate of stem cell differentiation. Many natural and synthetic materials are used as biological scaffolds, which regulate the differentiation of stem cells by acting as the extracellular matrix of st...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0797C12N5/0775C23C18/16
CPCC12N5/0623C12N5/0663C12N5/0665C12N5/0668C12N2533/20C12N2533/30C23C18/1648
Inventor 王舒李琳琳刘宏牟晓宁仇吉川郭伟博聂锦辉张晓笛刘志荣王中林
Owner BEIJING INST OF NANOENERGY & NANOSYST
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