Natural mixed phytosterol catechin, preparation method and application

A technology of plant sterols and catechins, which is applied in the fields of steroids, drug combinations, food science, etc., can solve the problems of poor solubility of free phytosterols and limit the scope of practical use, and achieve good fat-soluble antioxidant function, good resistance to Oxidation performance, inexpensive effect

Active Publication Date: 2020-05-22
ZHEJIANG UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the solubility of free phytosterols in oils is poor, which limits its practical application range

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Natural mixed phytosterol catechin, preparation method and application
  • Natural mixed phytosterol catechin, preparation method and application
  • Natural mixed phytosterol catechin, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Phytosterol (412.7mg), succinic anhydride (120.0mg) and potassium carbonate (200.0mg) were stirred and reacted at room temperature in dimethylformamide for 1 hour, and extracted with ethyl acetate after the reaction was completed, and the product obtained was mixed with Tetra-n-propionyl catechin (510.0mg) and N,N'-dicyclohexylcarbodiimide (415.0mg) were stirred and reacted in tetrahydrofuran at room temperature for 2 hours, and treated with hydrazine (300.0mg) after the reaction Then extracted with ethyl acetate and purified by chromatography to obtain natural mixed phytosterol catechins (65% yield).

[0039] The phytosterols used in this embodiment are purchased from the market, including 30% stigmasterol (stigmasterol), 5% brassicasterol (brassicasterol), 20% ergosterol (campesterol) and 40% β-sitosterol ( β-sitosterol), total purity 95%.

[0040] Structure determination data: IR 1734cm -1 The left and right sides show the characteristic absorption peaks of the est...

Embodiment 2

[0042] Phytosterol (412.7mg), succinic anhydride (160.0mg) and triethylamine (150.0mg) were stirred and reacted in dimethylformamide at room temperature for 1 hour, and extracted with ethyl acetate after the reaction was completed, and the obtained product React with tetra-n-propionyl catechin (620.0mg) and N,N'-dicyclohexylcarbodiimide (500.0mg) in tetrahydrofuran at room temperature for 2 hours. After treatment and extraction with ethyl acetate, natural mixed phytosterol catechins (yield 78%) were obtained after chromatographic purification.

Embodiment 3

[0044] Phytosterol (412.7mg), succinic anhydride (170.0mg) and sodium hydroxide (55.0mg) were stirred and reacted in dimethylformamide at room temperature for 1 hour, and extracted with ethyl acetate after the reaction was completed, and the obtained product was React with tetra-n-propionyl catechin (643.0mg) and N,N'-dicyclohexylcarbodiimide (560.0mg) in tetrahydrofuran at room temperature for 2 hours. After treatment and extraction with ethyl acetate, natural mixed phytosterol catechins were obtained after chromatographic purification (83% yield).

[0045] Antioxidant activity test: The fat-soluble antioxidant activity of natural mixed phytosterol catechins was tested using the classic ORAC method, and the results showed that the ORAC antioxidant activity of natural mixed phytosterol catechins was nearly 2 times that of catechins. The commonly used strong fat-soluble antioxidant TBHQ is 23% higher.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses natural mixing phytosterol catechin, a preparation method and applications thereof, wherein succinic anhydride and the hydroxyl groups of phytosterol and catechin form the ester group to link phytosterol and catechin so as to obtain the functional molecule. The invention further discloses a preparation method of the natural mixing phytosterol catechin, wherein the preparation method comprises: mixing succinic anhydride and phytosterol, adding an acid-binding agent, carrying out a reaction, adding protected catechin and a coupling reagent to the obtained product, stirring until completing the reaction, and carrying out post-treatment to obtain the natural mixing phytosterol catechin. According to the present invention, the natural mixing phytosterol catechin has advantages of good liposolubility, good anti-oxidation property, wide application range and simple use, and the preparation method can be used for industrial production.

Description

technical field [0001] The invention belongs to the field of functional food and health food, and in particular relates to a natural mixed phytosterol catechin molecule and its preparation method and application. Background technique [0002] Phytosterols are a class of natural components present in plants. A large number of animal experiments and human clinical trial data show that phytosterols have significant significance in reducing blood cholesterol levels and reducing the occurrence of cardiovascular diseases, and are an important health food ingredient. However, the solubility of free phytosterols in oils is poor, which limits its practical application range. Phytosterols are esterified with carboxylic acids to form phytosterol esters, which can significantly improve their solubility. The carbon chain length of fatty acids and the degree of saturation of fatty acids affect the melting point and fat solubility of phytosterol esters. By selecting the type and composit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & AuthorityPatents(China)
IPC IPC(8): C07J17/00A61P39/06A23L33/11
CPCC07J17/00
Inventor刘松柏吴珊珊夏方正
OwnerZHEJIANG UNIV