A method for producing acrylic acid by fermentation of Saccharomyces cerevisiae

A technology of Saccharomyces cerevisiae and Saccharomyces cerevisiae, which is applied in the field of biosynthesis of acrylic acid, can solve the problems of unclear synthesis mechanism, low synthesis efficiency, and complex synthesis pathways, and achieve good environmental and occupational health, easy production cost, and easy industrial transformation Effect

Active Publication Date: 2021-06-04
SHENZHEN INNOVATION CENT OF SMALL MOLECULE DRUG DISCOVERY CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Complicated synthetic pathways, unclear synthetic mechanisms, and extremely low synthetic efficiency are the shortcomings of existing biosynthetic methods for acrylic acid

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for producing acrylic acid by fermentation of Saccharomyces cerevisiae
  • A method for producing acrylic acid by fermentation of Saccharomyces cerevisiae
  • A method for producing acrylic acid by fermentation of Saccharomyces cerevisiae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0040] The technical solutions of the present invention will be further specifically described below through specific embodiments and in conjunction with the accompanying drawings. The principles and features of the present invention are described below, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0041] A method for producing acrylic acid by fermentation of Saccharomyces cerevisiae, the synthetic route of which is as follows figure 1 Said, the synthesis method uses glycerol as a raw material, Saccharomyces cerevisiae as a host bacterium, and is completed through the catalysis of several enzymes in the host bacterium; the host bacterium is constructed and obtained according to the following method:

[0042] 1) Optimize the codons of the genes GlyDH and DAK according to the codon preference of Saccharomyces cerevisiae, and then use the bidirectional promoter to construct the two genes...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
melting pointaaaaaaaaaa
boiling pointaaaaaaaaaa
flash pointaaaaaaaaaa
Login to view more

Abstract

The invention relates to a method for fermenting and producing acrylic acid by Saccharomyces cerevisiae. Glycerol is used as a raw material, Saccharomyces cerevisiae is used as a host bacterium, and the method is catalyzed by several enzymes in the host bacterium. Beneficial effects of the present invention: 1) low-cost compounds are used as raw materials, and the raw material cost of the process is low. 2) Based on the technology of transforming microorganisms, the catalytic synthesis of biological enzymes is carried out in microorganisms, avoiding large-scale extraction, separation and purification processes, and easy to control in terms of production costs and environmental friendliness. 3) This process is less than other methods in terms of scale and quantity of production equipment, simple operation, and easy industrial transformation. 4) This process only has a separation and purification process in the final step of production. The product purification process is simple, and the product quality is better than the general method with better purity and higher content. 5) There is no waste liquid discharge in the synthesis process, which is environmentally friendly, pollution-free, and sustainable production.

Description

technical field [0001] The invention relates to a biosynthesis method of acrylic acid, in particular to a method for producing acrylic acid by fermentation of Saccharomyces cerevisiae. Background technique [0002] Molecular formula of acrylic acid: C 3 h 4 o 2 ; English name: Acrylic acid; CAS: 79-10-7; The temperature is 141°C, the flash point is 54°C, and the density is 1.05g / cm. [0003] Acrylic acid is an important organic synthetic raw material and synthetic resin monomer, and is a vinyl monomer with a very fast polymerization rate. It is the simplest unsaturated carboxylic acid, consisting of a vinyl group and a carboxyl group. Pure acrylic acid is a clear, colorless liquid with a characteristic pungent odour. It is miscible with water, alcohol, ether and chloroform. Most of them are used to make acrylates such as methyl acrylate, ethyl ester, butyl ester, and hydroxyethyl ester. Acrylic acid and acrylate can be homopolymerized and copolymerized, and its polym...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/40C12N15/81C12R1/865
CPCC07K14/395C12N15/81C12P7/40
Inventor 卓炳照胡欣李棋林炜锋江会锋王文
Owner SHENZHEN INNOVATION CENT OF SMALL MOLECULE DRUG DISCOVERY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap