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A kind of culture method of high exopolysaccharide Lactobacillus San Francisco ls-1001 strain

A technology of Lactobacillus sanfrancisco and culture method, which is applied in the field of high-yielding exopolysaccharide Ls-1001 strain culture, can solve the problem that the characteristics of the strain and the culture conditions are not clearly understood, and achieve the effect of shortening the culture time and significantly increasing the production. Effect

Active Publication Date: 2021-07-27
SHANXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the industrial production of Lactobacillus San Francisco is not yet mature. The main reason is that the characteristics and culture conditions of the strain have not yet been clearly understood.

Method used

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  • A kind of culture method of high exopolysaccharide Lactobacillus San Francisco ls-1001 strain
  • A kind of culture method of high exopolysaccharide Lactobacillus San Francisco ls-1001 strain
  • A kind of culture method of high exopolysaccharide Lactobacillus San Francisco ls-1001 strain

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Experimental program
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Effect test

Embodiment 1

[0027] The present embodiment has selected five kinds of culture medium to carry out experiment respectively:

[0028] ①SDB medium: 6g / L peptone, 3g / L yeast powder, 20g / L maltose, 0.03% Tween 80 (3mL10% Tween / L), 1.5% fresh yeast extract, pH5.6;

[0029] Further, the preparation method of the fresh yeast extract is as follows: 20% commercially available high-activity dry yeast is dissolved in distilled water, sterilized under high pressure at 121°C for 30 minutes, cooled and placed overnight at 4°C, centrifuged and packaged, and stored at -80°C.

[0030] ②MRS medium: 10g / L peptone, 4g / L yeast powder, 5g / L beef extract powder, 20g / L glucose, 5g / L sodium acetate, 2g / L dipotassium hydrogen phosphate, 2g / L ammonium citrate, 0.2g / L magnesium sulfate, 0.05g / L manganese sulfate, 1mL / L Tween 80, pH5.4.

[0031] ③mMRS medium: 10g / L peptone, 5g / L yeast powder, 10g / L beef extract powder, 20g / L maltose, 5g / L sodium acetate, 2g / L dipotassium hydrogen phosphate, 2g / L ammonium citrate, 0.2...

Embodiment 2

[0036] The screening of embodiment 2 growth factors

[0037] On the basis of the optimal medium mMRS, two kinds of growth factors compounded with B vitamins and fresh yeast extract were studied, and the addition amount was 1.0g / L and 15mL / L respectively. The inoculum amount of the seed solution in the fermentation medium was 1%, the culture temperature was 30°C, and the initial pH was 5.4, and it was placed in an anaerobic incubator for static culture for 36 hours, and the number of colonies in the culture medium was measured.

[0038] figure 2 Show the impact of growth factors on the production of Lactobacillus San Francisco Ls-1001 bacterial classification, by figure 2 It can be seen that the complex B vitamins have a more significant effect on the growth of the strain than the fresh yeast extract, and the number of viable bacteria is as high as 2.77×10 8 CFU / mL. Therefore, complex B vitamins were selected as the growth-promoting factor of Lactobacillus San Francisco L...

Embodiment 3

[0039] The output of bacterial classification under embodiment 3 different times

[0040] image 3 Show the variation of Lactobacillus San Francisco Ls-1001 bacterial classification yield under different time, by image 3 It can be seen that the number of colonies tends to be stable when the culture time reaches 36 h. It can be concluded that the optimal culture time of Lactobacillus San Francisco Ls-1001 is 36 hours, which is significantly shortened from the 48 hours previously reported by Christiane B et al.

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Abstract

The invention discloses a method for cultivating a high-yield extracellular polysaccharide Lactobacillus San Francisco Ls-1001 strain, which comprises the following steps: diluting the frozen Lactobacillus San Francisco slant strain with sterilized physiological saline until it is measured at 600nm The absorbance value is A=0.5, and then inoculated into 100mL liquid medium supplemented with growth-promoting factors at a volume fraction of 2% for anaerobic culture. The pH of the medium is 5.0-5.4, and the culture temperature is 30-32°C. 34‑36 hours. The present invention increases the strain yield and exopolysaccharide yield of Lactobacillus San Francisco Ls-1001 by 4.6 times and 1.4 times respectively compared with the previous ones, shortens the culture time by 10-12 hours, has obvious production-increasing effect, and is also a good example for industrial production in the future Foundation.

Description

technical field [0001] The invention relates to a method for cultivating a high-yield extracellular polysaccharide Lactobacillus San Francisco Ls-1001 strain, belonging to food microorganisms [0002] field. Background technique [0003] Sourdough, generally known as "old noodles", "fermentation", "face fat" etc. in China, has a long history of use. In the 1980s, instant active dry yeast was introduced into my country. Because of its convenient use, the traditional pasta starter was gradually replaced. However, until now, it is still used in many areas, especially rural areas, to prepare steamed bread and other staple foods. Because of the use of traditional pasta The quality of the steamed bread prepared by the leavening agent is better. The reason is that the complex microbial system makes the fermented steamed bread have the best flavor and aroma, which has a good impact on the sensory quality. The application of Lactobacillus San Francisco has good effects on the flavo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/38C12R1/225
CPCC12N1/20C12N1/38
Inventor 张国华张纬珍刘俊峰孙瑜嵘何国庆
Owner SHANXI UNIV
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