Fast detection method of salmonella

A detection method and technology for Salmonella, applied in the field of Salmonella detection, can solve the problems of inconvenient rapid detection of Salmonella, and achieve the effects of saving detection time, simple operation steps and short cycle.

Inactive Publication Date: 2018-06-01
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the shortcoming that it is not convenient

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0028] A rapid detection method for salmonella, comprising the following steps:

[0029] S1. Sample treatment: Weigh 4g of solid sample and pulverize it, add sterile physiological saline to the pulverized solid sample to dissolve;

[0030] S2, prepare culture medium, culture medium is set as BPW medium;

[0031] S3. After the culture medium is cooled to room temperature, carry out ultraviolet sterilization on the culture medium for 17 minutes;

[0032] S4. Bacterial culture: put the sample to be tested into the culture medium and cultivate it for 5 hours, carry out bacterial identification on the cultured product, take the identified bacterial colony and add sterile physiological saline to prepare a bacterial suspension;

[0033] S5. Enrichment: Prepare enrichment medium: take 4.5g soytone, 29.0g magnesium chloride hexahydrate, 8.0g sodium chloride, 0.4g dipotassium hydrogen phosphate, 1L distilled water, and 0.6g potassium dihydrogen phosphate, mix them, and dissolve at a lo...

Embodiment 3

[0038] A rapid detection method for salmonella, comprising the following steps:

[0039] S1. Sample treatment: Weigh 3 g of solid sample and pulverize it, add sterile physiological saline to the pulverized solid sample for dissolution;

[0040] S2, prepare culture medium, culture medium is set as BPW medium;

[0041] S3. After the medium is cooled to room temperature, carry out ultraviolet sterilization on the medium for 20 minutes;

[0042] S4. Bacterial culture: put the sample to be tested into the culture medium and cultivate it for 5 hours, carry out bacterial identification on the cultured product, take the identified bacterial colony and add sterile physiological saline to prepare a bacterial suspension;

[0043] S5. Enrichment: Prepare enrichment medium: mix 4.5g soytone, 29.0g magnesium chloride hexahydrate, 8.0g sodium chloride, 0.4g dipotassium hydrogen phosphate, 1L distilled water, and 0.6g potassium dihydrogen phosphate, and dissolve at a low temperature , adjus...

Embodiment 4

[0048] A rapid detection method for salmonella, comprising the following steps:

[0049] S1. Sample treatment: Weigh 5 g of the solid sample and pulverize it, and add sterile physiological saline to the pulverized solid sample for dissolution;

[0050] S2, prepare culture medium, culture medium is set as BPW medium;

[0051] S3. After the medium is cooled to room temperature, carry out ultraviolet sterilization on the medium for 20 minutes;

[0052] S4. Bacterial culture: put the sample to be tested into the culture medium and cultivate it for 6 hours, carry out bacterial identification on the cultured product, take the identified bacterial colony and add sterile physiological saline to prepare a bacterial suspension;

[0053] S5. Enrichment: Prepare enrichment medium: mix 4.5g soytone, 29.0g magnesium chloride hexahydrate, 8.0g sodium chloride, 0.4g dipotassium hydrogen phosphate, 1L distilled water, and 0.6g potassium dihydrogen phosphate, and dissolve at a low temperature ...

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PUM

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Abstract

The invention provides a fast detection method of salmonella. The method comprises the following steps of S1, treating samples: weighing 2 to 5g of solid samples; performing crushing; adding sterile saline solution into the crushed solid samples for dissolution; S2, preparing a culture medium; S3, after the culture medium is cooled to the room temperature, performing ultraviolet sterilization on the culture medium for 15 to 20min; S4, performing bacterium culture: putting the samples to be tested into the culture medium to be cultured for 4 to 6h; performing bacterium identification on the cultured product; taking the identified bacterial colony, adding the sterile saline solution for preparing a bacterial suspension. The method provided by the invention has the advantages that the operation steps are simple and convenient; the period is shorter; the salmonella can be fast detected; the detection time is saved; the practical detection use is convenient.

Description

technical field [0001] The invention relates to the technical field of salmonella detection methods, in particular to a rapid detection method for salmonella. Background technique [0002] Salmonella is a zoonotic pathogen that seriously threatens the life and health of humans and animals. It can cause acute gastroenteritis, typhoid fever, sepsis, and extraintestinal focal infections in humans and animals. It has been listed as a Class A pathogenic microorganism by FAO. Over the years, food poisoning caused by Salmonella often ranks first in the list of bacterial food poisoning. my country's Center for Disease Control and Prevention has listed Salmonella as one of the bacterial indicators that require control in food. Accurate and timely detection methods are the key to preventing and controlling the spread of pathogenic microorganisms. The current detection methods for Salmonella are usually too cumbersome and take a long period of time. It usually takes many days to comp...

Claims

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Application Information

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IPC IPC(8): C12Q1/10C12R1/42
CPCC12Q1/045C12Q1/10G01N2333/255
Inventor 瞿孝云廖明张建民徐成刚
Owner SOUTH CHINA AGRI UNIV
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