Purpose of ion pump inhibitor for inhibiting epithelial-mesenchymal transition and resisting tumor metastasis

An anti-tumor metastasis and inhibitor technology, applied in the field of medicine, can solve problems such as severe infection, low immunity, metabolic disorders, etc., achieve the effect of inhibiting epithelial-mesenchymal transition and tumor metastasis, and huge social and economic benefits

Inactive Publication Date: 2018-06-05
LEADINGEN BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The low immunity of tumor patients leads to secondary severe infect

Method used

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  • Purpose of ion pump inhibitor for inhibiting epithelial-mesenchymal transition and resisting tumor metastasis
  • Purpose of ion pump inhibitor for inhibiting epithelial-mesenchymal transition and resisting tumor metastasis
  • Purpose of ion pump inhibitor for inhibiting epithelial-mesenchymal transition and resisting tumor metastasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Experimental materials and equipment: PC3 cell line ( CRL-1435 TM ), (Cat no114-07), dual chemiluminescent reporter system (pLenti-EcadPm-mCherry-PA-Spacer-VimPm-eGFP-PA-hPKG-Puro), small molecule compound library (Taosu).

[0052] Experimental steps:

[0053] 1. Transfect the double chemiluminescence system and verify the working efficiency

[0054] 1.1 Inoculate the single cell suspension of the human prostate cancer cell line PC3 evenly into a six-well plate, and when the cells grow to a density of about 80%, use (Cat no114-07) transfection reagent will double fluorescent reporter system pLenti-EcadPm-mCherry-PA-Spacer-VimPm-eGFP-PA-hPKG-Puro( figure 1 ) into PC3 cells, the specific transfection method refers to Transfection Reagent Instructions.

[0055] 1.2 After 72 hours of transfection, observe the cell state under a microscope. When the cell state is good, add 1ug / ml puromycin to the medium for cell selection.

[0056] 1.3 After 48 hours of puromycin ...

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Abstract

The invention provides a purpose of an ion pump inhibitor for inhibiting epithelial-mesenchymal transition and resisting tumor metastasis. The ion pump inhibitor has a general structure formula shownin the description; the effective concentration of the ion pump inhibitor is 20nm to 10mu m. An efficient dynamic screening method is used; various small molecule compounds are subjected to screeningwhether the small molecule compounds are EMT target spots or not; the obtained ion pump inhibitor can effectively inhibit epithelial-mesenchymal transition and tumor metastasis; a novel method is provided for treatment target spots for clinically treating tumor metastasis; huge social and economic interests can be brought.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of an ion pump inhibitor in inhibiting epithelial-mesenchymal transition and anti-tumor metastasis. Background technique [0002] With the rapid development of the global economy, science and humanities, human beings are paying more and more attention to the topic of global health. According to the statistical analysis of the global burden of disease by CBD in 2015, various diseases such as ischemic heart disease and cerebrovascular disease threaten human health, and many cancers such as lung cancer, liver cancer, colon cancer, and breast cancer also top the list, becoming human beings. One of the top killers. From the clear definition of cancer to the present, whether government agencies, researchers or civil organizations have invested decades in order to uncover the mystery of cancer and overcome cancer, but until recently, with economic development, populati...

Claims

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Application Information

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IPC IPC(8): A61K31/4439A61P35/04A61K31/337
CPCA61K31/337A61K31/4439A61K2300/00
Inventor 高维强朱鹤程姹萍
Owner LEADINGEN BIOTECH INC
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