Supercharge Your Innovation With Domain-Expert AI Agents!

A microfluidic chip-based typing method for human y-str gene loci

A technology of microfluidic chip and typing method, which is applied in the field of DNA analysis and can solve problems such as high temperature and long time

Active Publication Date: 2021-08-27
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, human STR genotyping in these studies still requires a long time, high temperature (≥60°) and different separation modes to achieve

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A microfluidic chip-based typing method for human y-str gene loci
  • A microfluidic chip-based typing method for human y-str gene loci
  • A microfluidic chip-based typing method for human y-str gene loci

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Electrophoresis Analysis of Y-STR Allele Typing Standards

[0021] 1) Selection of electrophoresis buffer system:

[0022] The injection buffer system consists of 50mmol / L tris(Tris), 50mmol / L N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid (TAPS), 1mmmol / L ethylenediaminetetra Acetic acid (EDTA) constitutes 1×TTE buffer solution, pH8.3; the sample separation system consists of POP 4 or POP 7 gel;

[0023] 2) Preparation of Y-STR allelic typing standards

[0024] Based on the consideration of genetic polymorphism (GD>0.6) and allele span in the Chinese population, we selected five Y chromosome loci with large polymorphism and small allele span in the Chinese population as research object. Under the condition of cooperation with Wuxi Zhongde Meilian Co., Ltd., a Y chromosome kit was jointly produced, and the loci included DYS459, DYS446, DYS443, DYS460 and Y-GATA-A10. The 5 loci were divided into two groups, HEX and TAMRA were used for group labeling, and the...

Embodiment 2

[0034] Electrophoretic Analysis of Y-STR Alleles in Blood Samples

[0035] 1) Electrophoresis buffer system

[0036] The injection buffer system consists of 50mmol / L tris(Tris), 50mmol / L N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid (TAPS), 1mmmol / L ethylenediaminetetra Acetic acid (EDTA) constitutes 1×TTE buffer solution, pH 8.3; the sample separation system consists of POP 4 or POP 7 gel.

[0037] 2) Preparation of Y-STR allelic typing standards

[0038] Based on the consideration of genetic polymorphism (GD>0.6) and allele span in the Chinese population, we selected five Y chromosome loci with large polymorphism and small allele span in the Chinese population as research object. Under the condition of cooperation with Wuxi Zhongde Meilian Co., Ltd., a Y chromosome kit was jointly produced, and the loci included DYS459, DYS446, DYS443, DYS460 and Y-GATA-A10. The 5 loci were divided into two groups, HEX and TAMRA were used to mark groups, and the molecular weigh...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for typing human Y-STR gene loci based on a microfluidic chip, and relates to the field of DNA analysis. The method comprises the steps of selecting a buffer system, selecting an electrophoresis chip, selecting a Y chromosome STR site, electrophoresis and the like. Based on chip electrophoresis technology combined with a laser-induced fluorescence detection system, the present invention separates the STR loci (DYS459, DYS446, DYS443, DYS460 and Y-GATA-A10) on five human Y chromosomes within 12 minutes, and completes the identification of real blood samples. Y-chromosome STR typing test. The high-resolution and rapid separation and analysis capabilities of chip electrophoresis for Y-chromosomal STR typing make it hopeful that it will become one of the analytical methods for fast, simple and efficient separation and detection of human chromosome STR loci.

Description

technical field [0001] The invention relates to the field of DNA analysis, in particular to a method for typing human Y-STR gene loci based on a microfluidic chip. Background technique [0002] Y chromosome (Y Chromosomes) is unique to males and has 60 million nucleotide fragments, which is only larger than chromosome 22 in the chromosome group. During meiosis, the Pseudoautosomal Regions (PAR) of the Y chromosome structure often exchange and recombine with the corresponding regions of the X chromosome, while the Y-specific regions (Nonrecombining Regions, NRY), which account for about 95% of the Y chromosome structure, do not. Recombination and exchange, showing haploid paternal inheritance, and retaining the mutation records of the paternal line, which is the theoretical basis for using the Y chromosome for forensic DNA analysis (Dupuy B M et al, Hum.Mutat., 2004, 23 (2): 117 -124). At the same time, due to its male-specificity, in actual case handling, when identifying ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6858
CPCC12Q1/6858C12Q2565/125C12Q2565/629C12Q2563/107
Inventor 秦建华苏文涛姜雷李艳峰
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com