Unlock instant, AI-driven research and patent intelligence for your innovation.
A microfluidic chip-based typing method for human y-str gene loci
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of microfluidic chip and typing method, which is applied in the field of DNA analysis and can solve problems such as high temperature and long time
Active Publication Date: 2021-08-27
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF9 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, human STR genotyping in these studies still requires a long time, high temperature (≥60°) and different separation modes to achieve
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0020] Electrophoresis Analysis of Y-STR Allele Typing Standards
[0021] 1) Selection of electrophoresis buffer system:
[0022] The injection buffer system consists of 50mmol / L tris(Tris), 50mmol / L N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid (TAPS), 1mmmol / L ethylenediaminetetra Acetic acid (EDTA) constitutes 1×TTE buffer solution, pH8.3; the sample separation system consists of POP 4 or POP 7 gel;
[0023] 2) Preparation of Y-STR allelic typing standards
[0024] Based on the consideration of genetic polymorphism (GD>0.6) and allele span in the Chinese population, we selected five Y chromosome loci with large polymorphism and small allele span in the Chinese population as research object. Under the condition of cooperation with Wuxi Zhongde Meilian Co., Ltd., a Y chromosome kit was jointly produced, and the loci included DYS459, DYS446, DYS443, DYS460 and Y-GATA-A10. The 5 loci were divided into two groups, HEX and TAMRA were used for group labeling, and the...
Embodiment 2
[0034] Electrophoretic Analysis of Y-STR Alleles in Blood Samples
[0035] 1) Electrophoresis buffer system
[0036] The injection buffer system consists of 50mmol / L tris(Tris), 50mmol / L N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid (TAPS), 1mmmol / L ethylenediaminetetra Acetic acid (EDTA) constitutes 1×TTE buffer solution, pH 8.3; the sample separation system consists of POP 4 or POP 7 gel.
[0037] 2) Preparation of Y-STR allelic typing standards
[0038] Based on the consideration of genetic polymorphism (GD>0.6) and allele span in the Chinese population, we selected five Y chromosome loci with large polymorphism and small allele span in the Chinese population as research object. Under the condition of cooperation with Wuxi Zhongde Meilian Co., Ltd., a Y chromosome kit was jointly produced, and the loci included DYS459, DYS446, DYS443, DYS460 and Y-GATA-A10. The 5 loci were divided into two groups, HEX and TAMRA were used to mark groups, and the molecular weigh...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a method for typing human Y-STR gene loci based on a microfluidic chip, and relates to the field of DNA analysis. The method comprises the steps of selecting a buffer system, selecting an electrophoresis chip, selecting a Y chromosome STR site, electrophoresis and the like. Based on chip electrophoresis technology combined with a laser-induced fluorescence detection system, the present invention separates the STR loci (DYS459, DYS446, DYS443, DYS460 and Y-GATA-A10) on five human Y chromosomes within 12 minutes, and completes the identification of real blood samples. Y-chromosome STR typing test. The high-resolution and rapid separation and analysis capabilities of chip electrophoresis for Y-chromosomal STR typing make it hopeful that it will become one of the analytical methods for fast, simple and efficient separation and detection of human chromosome STR loci.
Description
technical field [0001] The invention relates to the field of DNA analysis, in particular to a method for typing human Y-STR gene loci based on a microfluidic chip. Background technique [0002] Y chromosome (Y Chromosomes) is unique to males and has 60 million nucleotide fragments, which is only larger than chromosome 22 in the chromosome group. During meiosis, the Pseudoautosomal Regions (PAR) of the Y chromosome structure often exchange and recombine with the corresponding regions of the X chromosome, while the Y-specific regions (Nonrecombining Regions, NRY), which account for about 95% of the Y chromosome structure, do not. Recombination and exchange, showing haploid paternal inheritance, and retaining the mutation records of the paternal line, which is the theoretical basis for using the Y chromosome for forensic DNA analysis (Dupuy B M et al, Hum.Mutat., 2004, 23 (2): 117 -124). At the same time, due to its male-specificity, in actual case handling, when identifying ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6858
CPCC12Q1/6858C12Q2565/125C12Q2565/629C12Q2563/107
Inventor 秦建华苏文涛姜雷李艳峰
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Login to View More 
Login to View More