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Plants containing a low copy number of the ri gene
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A plant and gene technology, applied in the field of plant preparation, can solve the problems of delayed flowering and not obvious
Active Publication Date: 2022-01-21
KNUD JEPSEN
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Also, delayed flowering was less pronounced in line 331 than its counterpart purported to contain fewer copies of the rol gene
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[0141] plant material
[0142] The interspecific hybrid 2006-0199 (Knud Jepsen A / S, Hinnerup, Denmarkand AgroTech a / s, Denmark) in vivo plants at a temperature of 20°C during the day and night, a 16-hour day length, and 260 μmol photons m -2 the s -1 grown in a greenhouse under light intensity. The isolated plants were exposed to a temperature of 25 °C during the day and 22 °C at night, a day length of 13 h, and 75 μmol photon m -2 the s -1 cultured in a growth chamber under light intensity. Plants of other species were purchased from various suppliers.
[0143] Leaf explants of each species / hybrid were used for control experiments. Leaves derived from living material were sterilized in 70% EtOH for 1 min, then 20 min in 1% NaOCl (VWR, Copenhagen, Denmark) and 0.03% (v / v) Tween20 (Merck, La Jolla, USA), and Wash 3 times in sterile water and store until excision.
[0144] bacterial strain
[0145] Agrobacterium rhizogenes strain ATCC43057(A4) (kindly provided by Dr. M...
example
[0198] · Feather leaf longevity
[0199] · Edo Purple
[0200] · Palm pearl interspecific hybrid 'Tropical Parfait (Tropical Parfait)'
[0201] ·Disco kalanchoe
[0202] ·Tiger-patterned Kalanchoe
[0203] ·The interspecific hybrid 'Amazing Pink' of Kalanchoe lobata
[0204] It is mainly used as an ornamental plant at present. Reduced plant height and increased branching were observed compared to non-transformed plants. In addition, the content of secondary metabolites such as cyclohectorin A showing strong anti-tumor promoting activity makes this genus / species attractive as medicinal plants. Kalanchoe genus, especially Luna kalanchoe also contains coumaric acid, ferulic acid, syringic acid, caffeic acid, citric acid, isocitric acid, malic acid, p-hydroxybenzoic acid, flavonoids such as quercetin ( quercetin), camphenol, quercetin-3-diarabinoside (quercetin-3-diarabinoside), furanoside, quercetin-3-L-rhamnoside-L-arabinofuranoside (quercetin- 3-L-rhamnosido-L-arabino fu...
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Abstract
Plants transformed with one or more genes derived from the Ri plasmid of Agrobacterium rhizogenes, or progeny of such plants by infection with Agrobacterium rhizogenes containing the Ri plasmid, are described in Its genome contains 1 to 5 copies of the one or more genes derived from the Ri plasmid. Furthermore, the use of such plants or progeny thereof as ornamental plants, as field crop species, for food extracts, cosmetics, fragrances or as medicinal plants is disclosed. Methods for the preparation of such plants are further disclosed. The plants exhibit intermediate height and / or have a higher content of metabolites compared to control plants without the A. rhizogenes sequence, without significant reduction in flower number or delay in flowering time.
Description
technical field [0001] The present invention relates to, in particular of medium height, transformed with one or more genes derived from the Ri plasmid of A. rhizogenes by infection with Agrobacterium rhizogenes carrying the Ri plasmid. Plants, or progeny of such plants, and methods for preparing said plants. Background technique [0002] There is an increasing need for compact plant architectures in the field of ornamental plants. The potted plant industry favors denser plant structures for environmental, space and transportation purposes. Potted plants exhibiting elongated growth behavior are losing interest among breeders, plant producers, and consumers. [0003] Currently, the industry treats plants with growth retardants to limit plant size. Furthermore, plants transformed with A. rhizogenes have compact plant structures, wherein the length of the transformed plants is less than 20% of the height of the untransformed wild-type plants. Such transformants exhibit a va...
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