Tobacco explant anti-browning method based on active carbon and citric acid

A technology of explants and activated carbon, applied in the field of plant tissue culture, can solve the problems of insignificant effect, achieve good effect, improve differentiation state, and shorten the days of rooting

Pending Publication Date: 2018-06-12
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although these methods can play a certain anti-browning effect, the effect is not significant.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The tobacco explant anti-browning method based on gac and citric acid of the present embodiment may further comprise the steps:

[0029] Step (1), disinfection of seeds: take plump tobacco seeds, then move them to an ultra-clean workbench, soak the seeds with 75% alcohol for 30s, rinse them twice with sterile water, and then use 1% AgNO 3 Disinfect for 10 minutes, soak and wash in sterile water for 5 times, dry the surface moisture of seeds with sterile filter paper and then inoculate, wherein the sterile water is ultrapure water sterilized by autoclaving.

[0030] Step (2), seed cultivation: inoculate the seeds obtained in step (1) into MS medium, under the conditions of cultivation temperature of 25±1°C, light intensity of 30 μmol / (m2 s), and light time of 16 h / d Under culture for 60 days, the seeds germinate and grow into explant seedlings, and then coat the surface with water soaked in raw potatoes. The water soaked in raw potatoes contains substances such as starch...

Embodiment 2

[0037] The tobacco explant anti-browning method based on gac and citric acid of the present embodiment, wherein:

[0038] Steps (1)-(2) are the same as in Example 1;

[0039] Step (3), pre-cultivation of tobacco explants: transfer and inoculate the explant seedlings obtained in step (2) into a medium containing MS basic medium, 4g / L agar, 30g / L walnut kernels added with 10g / L L sucrose MS pre-cultured on solid medium. The control temperature is 24°C, the light intensity is 1500lux, the light time is 10 hours / day, and the culture time is 3 days. Finally, the pre-cultured explants are repeatedly inoculated in MS medium 4-5 times to make the tissue Partial infiltration of phenolic substances in the medium can promote the rapid germination of explants. Walnut grains are rich in melatonin, which can scavenge free radicals and prevent cell oxidative damage from browning.

[0040] Step (4) is identical with embodiment 1;

[0041] Step (5), explant anti-browning culture: place the e...

Embodiment 3

[0045] The tobacco explant anti-browning method based on gac and citric acid of the present embodiment, wherein:

[0046] Steps (1)-(2) are the same as in Example 1;

[0047] Step (3), pre-cultivation of tobacco explants: transfer and inoculate the explant seedlings obtained in step (2) into a medium containing MS basic medium, 4g / L agar, 30g / L walnut kernels added with 15g / L L sucrose MS pre-cultured on solid medium. The control temperature is 24°C, the light intensity is 1500lux, the light time is 10 hours / day, and the culture time is 3 days. Finally, the pre-cultured explants are repeatedly inoculated in MS medium 4-5 times to make the tissue Partial infiltration of phenolic substances in the medium can promote the rapid germination of explants. Walnut grains are rich in melatonin, which can scavenge free radicals and prevent cell oxidative damage from browning.

[0048] Step (4) is identical with embodiment 1;

[0049] Step (5), explant anti-browning culture: place the...

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PUM

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Abstract

The invention relates to a tobacco explant anti-browning method based on active carbon and citric acid. The method comprises the following steps: (1) seed disinfection; (2) seed culture; (3) tobacco explant pre-culture; (4) tobacco explant tissue culture; and (5) explant anti-browning culture. The method provided by the invention has the advantages that multiple anti-browning methods are effectively combined and are interactive, so that the anti-browning effect is better, and the rooting time is greatly shortened; the explant browning rate is 9.9-15.9%, and the explant can root in 7-10 days; and after the explant is cultured for 40-46 days, the root length is greater than 1cm, and the root number is greater than or equal to 2, so that the effect is good.

Description

technical field [0001] The invention relates to a method for preventing browning of tobacco explants, in particular to a method for preventing browning of tobacco explants based on activated carbon and citric acid, and belongs to the field of plant tissue culture. Background technique [0002] Tobacco is a crop with high economic value and occupies a huge economic market in our country. In addition to being mainly used for smoking, protein food and medicine, tobacco also has a wide range of uses. With the development of plant science, tobacco, as a model crop in basic research and application, has played an important role in the study of plant genetics, development, physiology, biochemistry and metabolism in this life. However, the browning problem of tobacco explants in the tissue culture process is common and almost unavoidable, so preventing browning is a problem to be solved in tobacco tissue culture. [0003] Explants with browning phenomenon can still grow and take r...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 曾婉俐邓乐乐向海英宋春满张涛许力张建铎蒋佳芮苏杨李雪梅陈章玉
Owner CHINA TOBACCO YUNNAN IND
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