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Enterococcus faecium strain and application thereof in alkekengi bean curd processing

A technology of Enterococcus faecium and strains, applied in the field of Enterococcus faecium strains and its application in the processing of physalis tofu, to achieve the effects of reducing the content of polluting pathogenic bacteria and miscellaneous bacteria, good stability, and reducing production costs

Active Publication Date: 2018-07-03
HUAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the application of Enterococcus faecium in the production of Physalis tofu

Method used

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  • Enterococcus faecium strain and application thereof in alkekengi bean curd processing
  • Enterococcus faecium strain and application thereof in alkekengi bean curd processing
  • Enterococcus faecium strain and application thereof in alkekengi bean curd processing

Examples

Experimental program
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Effect test

Embodiment 1

[0031] This example is the process of obtaining and screening Enterococcus faecium R2 strain. Its specific operation is as follows:

[0032] 1.1 strain screening

[0033] Weigh 10 g of kimchi samples and place them in a conical flask containing 100 mL of sterile saline, shake them well to form a suspension. Using aseptic technique, use sterile normal saline to dilute the above suspension to 10 -5 , 10 -6 、10 -7 times, coated with CaCO 3 MRS on solid medium. Then cultured at 37°C for 48h. Pick the colonies with calcium-dissolving circles and separate them by streaking on the plate, and repeat this 4-5 times until pure single colonies are obtained. Transfer the purified single colony to the sterilized pure yellow pulp water liquid medium, and culture it statically at 37°C for 72 hours, evaluate the fermentation performance of lactic acid bacteria by using the biomass of lactic acid bacteria and the pH of the fermentation broth as indicators, and screen a biological strain...

Embodiment 3

[0045] This example is a study on the growth characteristics of Enterococcus faecium R2 strain.

[0046] Inoculate the Enterococcus faecium R2 strain into fresh MRS liquid medium (100mL / 250mL Erlenmeyer flask), culture it statically at 37°C, measure its absorbance at 600nm, and draw the growth curve of the strain, as shown in figure 1 As shown, the time for the strain to reach the growth stationary phase was 16h.

Embodiment 4

[0048] This embodiment is an application method of a preferred form of Enterococcus faecium R2 strain in the processing of Physalis tofu, which includes the following steps:

[0049] Take fresh tofu yellow slurry water, pack in 250mL Erlenmeyer flasks, sterilize at 120°C for 15min. Cool to about 35°C and inoculate 3% (v / v) Enterococcus faecium R2 liquid seed solution (MRS medium), and culture at 37°C for 32 hours to obtain bean curd slurry with a pH of 4.0. The tofu physalis can be directly used as a coagulant for the physalis tofu. The soybeans are cleaned of impurities, cleared, soaked, beaten and slag-removed, and then boiled to obtain soybean milk. The obtained Physalis coagulant is added to the obtained cooked soybean milk in an amount of 16% (v / v) to obtain soybean brains, and the obtained soybean brains are poured into the pavement In the frame of good tofu cloth, add mesh cloth, cover flat, self-draining, and then pressurize to obtain Physalis tofu.

[0050] In the a...

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PUM

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Abstract

The invention relates to an enterococcus faecium strain. The 16S rDNA gene sequence is as shown in SEQ ID No.1. The strain is R2, the classification and naming of which is enterococcus faecium, the preservation number of which is CGMCC No.14944. The invention also relates to an application of the R2 in alkekengi bean curd processing. The application comprises the following steps: sterilization byyellow serofluid; inoculating R2 to the sterilized yellow serofluid; leaving the mixture to stand to ferment, wherein the pH value of the yellow serofluid reaches a preset value to obtain the alkekengi; adding the alkekengi into boiled soybean milk to obtain uncongealed beancurds, and carrying out follow-up treatment on the uncongealed beancurds to obtain alkekengi bean curds. The enterococcus faecium strain is simple in culture condition, high in acid-yielding speed, and good in stability; the alkekengi bean curds are produced by means of the strain, so that the content of polluting pathogenic bacterium and infectious microbes can be reduced effectively, and in the fermenting process, nutrients are not supplemented, and meanwhile, after fermentation, a proper acidity can be achieved without supplementing acetic acid, so that the production cost is lowered effectively.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to an Enterococcus faecium strain and its application in processing Physalis tofu. Background technique [0002] Yellow pulp water is a by-product produced during tofu processing. It contains nutrients such as soybean isoflavones, protein and vitamins, as well as a large amount of water-soluble sugars such as stachyose, raffinose and other oligosaccharides, which can be fermented and utilized by microorganisms. Direct discharge in the production process not only causes a waste of resources, but also causes serious pollution to the surrounding environment. In our country, the folks use yellow pulp water to ferment and produce acid under natural conditions to obtain Physalis coagulant and make Physalis tofu. Compared with tofu with gypsum and brine as coagulants, Physalis tofu has the characteristics of fine texture, good water retention, and fresh taste, and is deeply loved by peop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C20/02C12R1/46A23L11/45A23L11/50
CPCA23C20/02C12R2001/46C12N1/205
Inventor 陈志娜张科贵叶韬丁曼黄琳
Owner HUAINAN NORMAL UNIV
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