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Application of TMTC1 gene as novel molecular marker

A gene and gene expression technology, which is applied in the field of chronic obstructive pulmonary disease diagnostic kits and preparation of chronic obstructive pulmonary disease diagnostic kits, can solve the problems of the overall prevalence of chronic obstructive pulmonary disease and achieve a favorable diagnosis Effect

Inactive Publication Date: 2018-07-20
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are vertical differences in the survey data, it can still be seen that the overall prevalence of COPD is grim

Method used

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  • Application of TMTC1 gene as novel molecular marker
  • Application of TMTC1 gene as novel molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Screening of differentially expressed genes between patients with chronic obstructive pulmonary disease and normal people

[0033] 1. Clinical research object:

[0034] Five patients with chronic obstructive pulmonary disease were selected, including 2 males and 3 females, aged from 50 to 79 years old. The diagnostic criteria were all in line with the "Code for Diagnosis and Treatment of Chronic Obstructive Pulmonary Disease" revised in 2007 in my country.

[0035] Diagnostic criteria: COPD can be diagnosed in any patient with dyspnea, chronic cough, or phlegm production, with a history of exposure to risk factors, and pulmonary function tests showing airflow limitation following inhaled bronchodilators.

[0036] Exclusion criteria: ①Combined with other lung diseases, such as bronchial asthma, interstitial pulmonary fibrosis, lung cancer, etc.; ②Patients with infection in other parts; organ failure, hepatitis; ④ suffering from immune system diseases or recent...

Embodiment 2

[0076] Example 2 QPCR verification of the relationship between candidate genes and chronic obstructive pulmonary disease

[0077] Based on the previous high-throughput sequencing results, we selected the TMTC1 gene (its expression is upregulated in patients with chronic obstructive pulmonary disease) for verification according to the size of the P value.

[0078] 1. Research object:

[0079] According to the method of Example 1, 45 patients with chronic obstructive pulmonary disease and 35 normal people were selected.

[0080] 2. Total RNA extraction from blood

[0081] Use Biotech Blood RNA Extraction Kit to extract total RNA from blood:

[0082] (1) Take 250 μl (or 0.25 g) of whole blood into an RNase-Free filter column, centrifuge at 13,000 rpm for 2 minutes, collect the lower liquid, and add 0.75 ml of lysis solution RLS.

[0083] (2) Shake the homogenate sample vigorously, and incubate at 15-30°C for 5 minutes to completely decompose the ribosomes.

[0084] (3) Optional...

Embodiment 3

[0100] Example 3 Western blot experiment to verify the expression products of differentially expressed genes in patients with chronic obstructive pulmonary disease and normal people

[0101] 1. Clinical object: same as embodiment 2.

[0102] 2. Mononuclear cell isolation

[0103] Patients with chronic obstructive pulmonary disease and normal people take 10ml of venous blood, inject it into a sterile vial containing heparin, and shake it gently immediately after capping. Add an equal volume of HBSS (NaCl 8.0g, NaCl 2 HPO 4 0.132g, KH 2 PO 4 0.06g, KCl 0.4g, phenol red 1ml, NaHCO 3 0.35g, D-glucose 1.0g, dissolved in 1000ml double distilled water), to reduce the aggregation of red blood cells. Draw 8ml of lymphocyte stratification solution into a 50ml centrifuge tube, slowly add the diluted blood along the tube wall, keep the interface clear, do not mix the two, centrifuge at 20°C 2000r / min for 30min, carefully absorb the stratification solution and plasma transfer The ...

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Abstract

The invention discloses application of a TMTC1 gene in diagnosis of chronic obstructive pulmonary diseases. The content of the TMTC1 gene in the blood of a patient suffering from the chronic obstructive pulmonary disease obviously differs from that of a normal person, and the patient suffering from the chronic obstructive pulmonary disease and the normal person can be distinguished according to the difference. The invention further discloses a diagnosis product for the chronic obstructive pulmonary diseases, and the diagnosis product can fulfill the purpose of diagnosing the chronic obstructive pulmonary diseases by detecting the TMTC1 gene in the blood.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a diagnostic kit for chronic obstructive pulmonary disease and the use of TMTC1 gene in preparing the diagnostic kit for chronic obstructive pulmonary disease. Background technique [0002] Chronic obstructive pulmonary disease (COPD for short) is a common airflow-limited respiratory disease. The airflow limitation usually develops progressively, accompanied by chronic airway inflammation after inhalation of harmful gases or particles. The main clinical manifestations are dyspnea, chronic cough, and expectoration (Chronic Obstructive Pulmonary Disease Group, Respiratory Diseases Branch of Chinese Medical Association, Guidelines for Diagnosis and Treatment of Chronic Obstructive Pulmonary Disease (2013 revision) [J], Chinese Journal of Tuberculosis and Respiratory Medicine , 2014, 36(2): 8-17). [0003] Worldwide, the prevalence of COPD varies greatly across countries. A survey of peopl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68
CPCC12Q1/6883C12Q2600/158G01N33/6893G01N2333/47G01N2800/122G01N2800/50
Inventor 向常娟马翠
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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