Immunofluorescence chromatography detection card used for detecting canine coronavirus antigen, and preparation method

A coronavirus and immunofluorescence technology, which is applied in the field of immunofluorescence chromatography detection card, can solve the problems of inappropriate on-site detection, non-dry detection card, and inconvenient operation, so as to achieve good immune reaction efficiency, reduce detection cost, reduce workload effect

Pending Publication Date: 2018-07-24
广州敏捷生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the following problems: the fluorescently labeled canine coronavirus antibody is divided into reagent bottles, rather than directly sprayed on the marker pad, so that the whole detection card is not dry and needs to be refrigerated. The sample solution is added to the reagent bottle, the operation is not convenient, and it is not suitable for on-site detection

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  • Immunofluorescence chromatography detection card used for detecting canine coronavirus antigen, and preparation method
  • Immunofluorescence chromatography detection card used for detecting canine coronavirus antigen, and preparation method
  • Immunofluorescence chromatography detection card used for detecting canine coronavirus antigen, and preparation method

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Embodiment 1

[0059] A kind of immunofluorescence chromatography detection card for detecting canine coronavirus antigen provided by the present invention, refer to Figure 1 to Figure 2 , including a housing 7, the housing 7 is provided with an immunofluorescence chromatography detection card for detecting canine coronavirus antigen, and the immunofluorescence chromatography detection card for detecting canine coronavirus antigen includes a bottom plate 1, in The bottom plate 1 is sequentially connected with a sample pad 2, a binding pad 3, a nitrocellulose membrane 4 and a water-absorbing pad 5, and the binding pad 3 is provided with fluorescent microsphere-labeled CCV monoclonal antibody 31 and fluorescent microsphere-labeled goat anti-chicken IgY32 , the nitrocellulose membrane 4 is provided with a quality control C line coated with chicken IgY, and a detection T line coated with CCV monoclonal antibody. The housing 7 is provided with a sample injection hole 72 compatible with the sampl...

Embodiment 2

[0061] Another immunofluorescence chromatography test card for detecting canine coronavirus antigen provided by this application, see Figure 1 to Figure 3 , including a box body 9, the box body 9 is provided with a sample diluent bottle 6, a suction pipe 8 and an immunofluorescence chromatography detection card for detecting the canine coronavirus antigen, and the immunofluorescence chromatography detection card for detecting the canine coronavirus antigen The fluorescence chromatography detection card is located in the housing 7, and the immunofluorescence chromatography detection card for detecting the canine coronavirus antigen includes a base plate 1, and the base plate 1 is connected with a sample pad 2, a binding pad 3, and a nitrocellulose membrane 4 in sequence And water-absorbing pad 5, described binding pad 3 is provided with fluorescent microsphere mark CCV monoclonal antibody 31 and fluorescent microsphere mark goat anti-chicken IgY32, and described nitrocellulose ...

Embodiment 3

[0063] The preparation method of the immunofluorescence chromatography detection card for detecting the canine coronavirus antigen provided by embodiment 1 and embodiment 2 is assembled under the environment of temperature (18~26) ℃ and humidity≤30%, on the bottom plate 1 The nitrocellulose membrane 4, the water-absorbing pad 5, the binding pad 3 and the sample pad 2 are successively connected; cut the pasted large plate into 4.0MM wide test strips, and put them into the plastic card case, that is, the CCV test card; The test card is placed in an aluminum film bag, add 1 pack of desiccant, and heat seal it for later use.

[0064] The specific preparation method is as follows:

[0065] 1.1 Preparation of conjugation pads

[0066] 1.1.1 Labeling of CCV monoclonal antibody

[0067] 1) Select microspheres: microspheres are polystyrene microspheres containing fluorescent mismatches of rare earth europium elements; density: 1.05g / cm3; refractive index: 1.59@589nm, 25°C; diameter 3...

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Abstract

The invention discloses an immunofluorescence chromatography detection card used for detecting a canine coronavirus antigen, and a preparation method, and aims to provide the immunofluorescence chromatography detection card which has the advantages of high sensitivity and reliable detection result and is used for detecting the canine coronavirus antigen. The immunofluorescence chromatography detection card has the technical key points that the detection card comprises a bottom plate, wherein a sample cushion, a combination cushion, a nitrocellulose membrane and a water adsorption cushion are connected in sequence onto the bottom plate; the combination cushion is provided with a CCV (canine coronavirus) monoclonal antibody labeled by fluorescent microspheres and goat anti-chicken IgY labeled by the fluorescent microspheres; the nitrocellulose membrane is provided with the quality control C line of coating chicken IgY, and the detection T line of the coating CCV monoclonal antibody. Theimmunofluorescence chromatography detection card belongs to the field of animal epidemic diseases.

Description

technical field [0001] The invention discloses an immunofluorescence chromatography detection card, specifically, an immunofluorescence chromatography detection card for detecting canine coronavirus antigen, and the invention also discloses a preparation method of the immunofluorescence chromatography detection card . Background technique [0002] The pathogen of canine coronavirus disease is canine coronavirus (Canine Corona Virus, CCV). It is clinically characterized by gastroenteritis of varying severity and clinically fatal watery diarrhea. Sudden onset, depression, loss of appetite, vomiting, discharge of foul-smelling soft and mucous feces. Vomiting often lasts for several days and does not subside until diarrhea develops. Afterwards, the feces range from semen-like, semi-pasty to watery, orange or green, and contain mucus and varying amounts of blood. Rapid dehydration, weight loss. The source of infection is mainly sick dogs and infected dogs. The detoxification...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/577G01N33/533
CPCG01N33/533G01N33/56983G01N33/577
Inventor 汤永平梁展鹏
Owner 广州敏捷生物技术有限公司
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