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Immunofluorescence chromatography detection card for detecting canine distemper virus antigen and preparation method thereof

A canine distemper virus and immunofluorescence technology, applied in the field of immunofluorescence chromatography detection cards, can solve the problems of failure to disclose the dose-response curve of reagents, rapid diagnosis and treatment of unfavorable diseases, cumbersome and time-consuming operations, etc. The effect of fast speed and reduced workload

Pending Publication Date: 2018-11-20
广州敏捷生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the linear range of the detection kit is not high, and the patent fails to disclose the dose-response curve of the reagent; horseradish peroxidase is used to label the goat anti-mouse antibody, and the specificity may be affected
In addition, the whole operation is very cumbersome and time-consuming, requiring a series of steps such as sample dilution, sample addition, washing, adding enzyme-labeled goat anti-mouse antibody, washing, adding luminescent liquid, and detection, which is not conducive to early and rapid diagnosis and treatment of diseases

Method used

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  • Immunofluorescence chromatography detection card for detecting canine distemper virus antigen and preparation method thereof
  • Immunofluorescence chromatography detection card for detecting canine distemper virus antigen and preparation method thereof
  • Immunofluorescence chromatography detection card for detecting canine distemper virus antigen and preparation method thereof

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Embodiment 1

[0060] A kind of immunofluorescence chromatography detection card for detecting canine distemper virus antigen provided by the present invention, see Figure 1 to Figure 2 , including a housing 7, the housing 7 is provided with an immunofluorescence chromatography detection card for detecting canine distemper virus antigen, and the immunofluorescence chromatography detection card for detecting canine distemper virus antigen includes a bottom plate 1 A sample pad 2, a binding pad 3, a nitrocellulose membrane 4 and a water-absorbing pad 5 are sequentially connected on the bottom plate 1, and the binding pad 3 is provided with a fluorescent microsphere-labeled CDV monoclonal antibody 31 and a fluorescent microsphere-labeled sheep Anti-chicken IgY32, the nitrocellulose membrane 4 is provided with a quality control C line coated with chicken IgY, and a detection T line coated with CDV monoclonal antibody. The housing 7 is provided with a sample injection hole 72 compatible with the...

Embodiment 2

[0062] Another immunofluorescence chromatography detection card for detecting canine distemper virus antigen provided by this application, see Figure 1 to Figure 3 , including a box body 9, the box body 9 is provided with a sample diluent bottle 6, a suction pipe 8 and an immunofluorescence chromatography detection card for detecting the canine distemper virus antigen, and the described method for detecting the canine distemper virus antigen The immunofluorescence chromatography detection card is located in the housing 7, and the immunofluorescence chromatography detection card for detecting canine distemper virus antigen includes a base plate 1, on which the sample pad 2, the binding pad 3, the nitric acid Fiber membrane 4 and water-absorbing pad 5, the CDV monoclonal antibody 31 of fluorescent microsphere mark and the goat anti-chicken IgY32 of fluorescent microsphere mark are arranged on the described binding pad 3, and described nitrocellulose membrane 4 is provided with a...

Embodiment 3

[0064] The preparation method of the immunofluorescence chromatography detection card for detecting canine distemper virus antigen provided by embodiment 1 and embodiment 2 is assembled under the environment of temperature (18~26) ℃ and humidity ≤30%, on the bottom plate 1 Nitrocellulose membrane 4, water-absorbing pad 5, binding pad 3, and sample pad 2 are successively connected on the top; cut the pasted large board into 4.0mm wide test strips, and put them into the plastic card case, that is, the CDV test card; One test card is placed in an aluminum film bag, 1 pack of desiccant is added, and the seal is heat-sealed for later use.

[0065] The specific preparation method is as follows:

[0066] 1.1 Preparation of conjugation pads

[0067] 1.1.1 Labeling of CDV monoclonal antibody

[0068] 1) Select microspheres: microspheres are polystyrene microspheres containing fluorescent mismatches of rare earth europium elements; density: 1.05g / cm3; refractive index: 1.59@589nm, 25°...

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Abstract

The invention discloses an immunofluorescence chromatography detection card for detecting a canine distemper virus antigen and a preparation method thereof. The invention aims to provide the immunofluorescence chromatography detection card for detecting the canine distemper virus antigen with high sensitivity and a reliable detection result. The technical point involves a bottom plate; a sample pad, a bonding pad, a nitrocellulose membrane and a water absorbing pad are sequentially connected on the bottom plate; the bonding pad is provided with a fluorescent microsphere-labeled CDV monoclonalantibody and a fluorescent microsphere-labeled goat anti-chicken IgY; and the nitrocellulose membrane is provided with a quality control C line coated with a chicken IgY, and a detection T line coatedwith the CDV monoclonal antibody. The immunofluorescence chromatography detection card for detecting the canine distemper virus antigen and the preparation method thereof belong to the field of animal disease detection.

Description

technical field [0001] The invention discloses an immunofluorescence chromatography detection card, specifically, an immunofluorescence chromatography detection card for detecting canine distemper virus antigen, and the invention also discloses the preparation of the immunofluorescence chromatography detection card method. Background technique [0002] Canine distemper (CD) is an acute, high-contact viral infectious disease of dogs that occurs widely worldwide. Hosts include most carnivores. [0003] Early biphasic fever, acute nasal catarrh followed by bronchitis, catarrhal pneumonia, severe gastroenteritis, and neurologic symptoms were characterized by hyperkeratosis of the nose and footpads of some affected animals. It is also highly contagious and has various clinical symptoms. It is prone to mixed infection or secondary infection of secondary bacteria and other viruses. sequelae. Routine immunization is a highly effective preventive protection measure under normal c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/533G01N33/577
CPCG01N33/533G01N33/56983G01N33/577
Inventor 汤永平梁展鹏
Owner 广州敏捷生物技术有限公司
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