Hybridoma cell line, cd68 monoclonal antibody, preparation method and application

A hybridoma cell line and monoclonal antibody technology, applied in the field of biomedicine, can solve the problems of high preparation cost and poor stability of monoclonal antibodies, and achieve the effects of high specificity and sensitivity, and improved accuracy
CN108330107BActive Publication Date: 2021-10-29富恩生物技术(成都)有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
富恩生物技术(成都)有限公司
Publication Date
2021-10-29

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Abstract

The present invention relates to a hybridoma cell strain, CD68 monoclonal antibody, preparation method and application. The monoclonal antibody uses purified CD68 recombinant extracellular region protein as an immunogen to immunize animals; B lymphocytes of the animal body are fused to obtain hybridoma cells; positive clones of specific hybridoma cells are screened, cell cloning is performed on the positive clones, and hybridoma cells stably secreting CD68 monoclonal antibody are screened out. It has the characteristics of strong specificity, high affinity, good stability and high potency.
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Description

technical field

[0001] The present invention relates to the field of biomedical technology, and more specifically, relates to a hybridoma cell line capable of producing CD68 monoclonal antibody, CD68 monoclonal antibody, and the preparation method and application of the antibody. Background technique

[0002] CD68 is a 110KDa glycoprotein associated with lysosomal granules. CD68 is a marker of macrophages in many human tissues, including bone marrow, spleen, intestinal lamina propria and alveoli. Peripheral blood monocytes are also positive, while bone marrow precursors and peripheral blood granulocytes can also be studied. In recent years, it has been reported in the literature that CD68 can also be used as a marker of histiocytes, mainly for the study of macrophages, acute and chronic myeloid leukemia, and tumors derived from histiocytes such as malignant fibrous histiocytoma.

[0003] With the deepening of tumor immunology research, it is known that the cells infiltrati...

Claims

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