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Pharmaceutical compositions useful for the treatment of tissue injury

A technology of tissue damage and composition, which is applied in the field of pharmaceutical composition for the treatment of tissue damage, and can solve the problems of discomfort and increased risk of specimens, and weakened effects of treatment plans, etc.

Pending Publication Date: 2018-08-03
美德睿臻有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the stem cell mobilization under the above-mentioned scheme is better than the effect seen when only using stem cell mobilizing agents or immunosuppressants, the stem cell mobilizing agents and immunosuppressants still need to inject multiple drugs at multiple sites, and the discomfort of the specimen and Increased risk with diminished effectiveness of the treatment regimen in question

Method used

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  • Pharmaceutical compositions useful for the treatment of tissue injury
  • Pharmaceutical compositions useful for the treatment of tissue injury
  • Pharmaceutical compositions useful for the treatment of tissue injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1、2

[0129] Embodiment 1,2 materials and methods:

[0130] Injectable AF Compositions

[0131] Reagent : AMD3100 with tacrolimus (powder) was obtained from Sigma-Aldrich (St. Louis, MO).

[0132] tacrolimus preparation : Due to the hydrophobicity of tacrolimus powder and its poor solubility in aqueous solution (such as normal saline), tacrolimus powder is dissolved in 100% ethanol (8% of the total volume), castor oil (2% of the total volume) %) and sterile saline for injection (90% of the total volume). The experimental formula of tacrolimus is C 44 h 69 NO 12 •H 2 O, the relative molecular weight is 822.03.

[0133] AMD3100 formulation : 24 mg AMD3100 dissolved in sterile water containing 5.9 mg sodium chloride, pH adjusted to 6.0-7.5 with hydrochloric acid and, if necessary, sodium hydroxide. The molecular weight of AMD3100 is 502.79 g / mol.

[0134] AF composition : Add dissolved tacrolimus to AMD3100 solution, the weight ratio of tacrolimus to AMD3100 is abou...

Embodiment 1

[0135] Example 1 - Stem cell mobilization activity test of AF composition

[0136] Hematopoietic colony-forming cell (CFC) assays have been widely used in research and clinical applications in human and animal models to quantify and evaluate the content of hematopoietic progenitor cells in cell samples. The stem cell mobilization activity can be detected by CFC test of the mobilized peripheral blood samples. CFCs can divide and differentiate into more mature populations of cells detectable by light microscopy. This allows quantification of pluripotent stem cell lineages mobilized by pharmaceutical agents.

[0137] C57 / B6 mice were divided into 4 treatment groups: 1) control group treated with normal saline; 2) AMD3100 group (1.0 mg / kg); 3) tacrolimus treated with low dose (0.1 mg / kg) Group; 4) AF composition (including AMD3100 and tacrolimus). Animals were dissected 3 hours after drug treatment, peripheral blood was collected, and peripheral blood mononuclear cells (PBMCs...

Embodiment 2

[0139] Example 2 - AF composition promotes burn wound healing in mice

[0140] Stem cell therapy can improve the quality of burn wound healing, reduce scar formation, and rebuild the skin. To avoid the costly and time-consuming preparation of hematopoietic stem cells required in the treatment of burns, this study shows that endogenous bone marrow stem cells can be pharmacologically mobilized by AF combinations to treat burns.

[0141] C57 / B6 mouse back skin caused full-thickness burns (diameter 12mm) ( figure 2 ). Burned mice were randomly divided into 4 experimental groups as follows, and received subcutaneous injection of normal saline or drugs immediately after the wound surface was formed until healing: (1) the control group treated with normal saline; (2) treated every other day (1.0 mg / kg ) AMD3100 group; (3) FK506 group treated with low dose (0.1 mg / kg) every day; and (4) AF composition group treated every other day. All wound assessments were double-blind.

[01...

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Abstract

The present invention relates to the field of tissue injury. More specifically, the present invention provides pharmaceutical compositions useful for the treatment of tissue injury. In certain embodiments, a pharmaceutical composition comprises (a) a stem cell mobilizer and (b) an immunosuppressive agent or non-immunosuppressive FK binding protein ligand. The pharmaceutical compositions of the invention can be administered by a variety of routes and dosing regimens.

Description

field of invention [0001] The present invention relates to the field of tissue damage. In particular, the present invention provides pharmaceutical compositions for treating tissue damage. Background of the invention [0002] Stem cell therapy can be used in the treatment of a variety of conditions, including organ transplantation, a variety of tissue injuries (including trauma such as burns, surgery, pressure sores, skin ulcers), nerve injury and / or degeneration (including spinal cord injury), or the treatment of IBD or Diagnosis of other inflammatory or autoimmune diseases or occasional IBD or other inflammatory or autoimmune diseases. However, this therapy generally requires that endogenous stem cells be separated from the sample, cultured outside the sample, the stem cells prepared, and then reintroduced into the sample. The process described above is costly, time-consuming, and not always reliable. [0003] Existing studies have shown that stem cell mobilization agen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/19A61K31/395
CPCA61K45/06A61K31/395A61K31/4427A61K31/506A61K9/0019A61K47/10A61K9/08A61K47/44A61K31/436A61P17/02A61P1/00A61P29/00A61K2300/00
Inventor 孙兆立
Owner 美德睿臻有限公司
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