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A kind of extraction method of total alkaloids of Dajin flower

A technology of total alkaloids and extraction methods, which is applied in the direction of alkaloids, alkaloids, chemical instruments and methods, etc., can solve the problems of unsatisfactory extraction rate and not many alkaloids in datura, so as to facilitate extraction and separation, guarantee Sufficient dissolution and guaranteed solubility

Inactive Publication Date: 2021-04-06
HEBEI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the research, it was found that the extraction rate is not ideal, and there are not many studies on how to improve the extraction rate of alkaloids in datura in recent years

Method used

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  • A kind of extraction method of total alkaloids of Dajin flower

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Experimental program
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Effect test

Embodiment 1

[0022] A method for extracting total alkaloids of Datura japonica, comprising the steps of:

[0023] (1) Pulverize 100g of Yangjinhua, pass through an 80-mesh sieve, and set aside;

[0024] (2) Mix 5.5g of 1-butyl-3-methylimidazole lactate, 4g of PEG400 and 200g of deionized water, and adjust the pH to 3.5 with lactic acid to obtain an acidic extract;

[0025] (3) Add the acidic extract obtained in step (2) to the treated datura in step (1), add 1.2g of acid cellulase and 0.7g of acid pectinase, mix well, and keep the temperature at 50 Stir between ℃ for 2.5h, filter, wash the filter residue with pH 2.5 hydrochloric acid aqueous solution, and combine the filtrates;

[0026] (4) Add 1% ammonium sulfate and 2%NaCl in the filtrate, extract 3 times with 2 times volume of ethyl acetate of the filtrate respectively, remove the ethyl acetate phase, adjust the pH to 10 with ammonia water in the alkaloid-rich phase, then use 1.5 times the volume of chloroform was extracted three time...

Embodiment 2

[0028] A method for extracting total alkaloids of Datura japonica, comprising the steps of:

[0029] (1) Pulverize 100g of Yangjinhua, pass through an 80-mesh sieve, and set aside;

[0030] (2) Mix 3g of 1-butyl-3-methylimidazole lactate, 2g of PEG800 and 200g of deionized water, and adjust the pH to 3.5 with lactic acid to obtain an acidic extract;

[0031] (3) Add the acidic extract obtained in step (2) to the treated datura in step (1), add 1.2g of acid cellulase and 0.7g of acid pectinase, mix well, and keep the temperature at 50 Stir between ℃ for 2.5h, filter, wash the filter residue with pH 2.5 hydrochloric acid aqueous solution, and combine the filtrates;

[0032] (4) Add 1% ammonium sulfate and 2%NaCl in the filtrate, extract 3 times with 2 times volume of ethyl acetate of the filtrate respectively, remove the ethyl acetate phase, adjust the pH to 10 with ammonia water in the alkaloid-rich phase, then use 1.5 times the volume of chloroform was extracted three times,...

Embodiment 3

[0034] A method for extracting total alkaloids of Datura japonica, comprising the steps of:

[0035] (1) Pulverize 100g of Yangjinhua, pass through an 80-mesh sieve, and set aside;

[0036] (2) Mix 8g of 1-butyl-3-methylimidazole lactate, 6g of PEG1000 and 200g of deionized water, and adjust the pH to 3.5 with lactic acid to obtain an acidic extract;

[0037] (3) Add the acidic extract obtained in step (2) to the treated datura in step (1), add 1.2g of acid cellulase and 0.7g of acid pectinase, mix well, and keep the temperature at 40 Stir between ℃ for 2.5h, filter, wash the filter residue with pH 2.5 hydrochloric acid aqueous solution, and combine the filtrates;

[0038] (4) Add 1% ammonium sulfate and 2%NaCl in the filtrate, extract 3 times with 2 times volume of ethyl acetate of the filtrate respectively, remove the ethyl acetate phase, adjust the pH to 10 with ammonia water in the alkaloid-rich phase, then use Chloroform was extracted 3 times, chloroform was recovered u...

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Abstract

The present invention relates to a method for extracting total alkaloids of datura, which comprises the following steps: pulverizing 100g of datura and sieving for later use; 3~8g of 1-butyl-3-methylimidazole lactate, 2~ 6 Mix PEG300~1000 with 200g deionized water, adjust the pH to 3~5 with lactic acid to obtain an acidic extract; add the acidic extract to Dajinhua, add 0.8~1.5g acid cellulase and 0.8g acid pectinase, mix evenly, keep the temperature between 35~55℃ and stir for 1~4h, filter, wash the filter residue with hydrochloric acid aqueous solution with pH2~3, combine the filtrate; add 1% ammonium sulfate and 2% Add NaCl, add ethyl acetate to extract 3 times, remove the ethyl acetate phase, adjust the pH of the alkaloid-rich phase to 9~11 with ammonia water, then extract 3 times with chloroform, recover the chloroform under reduced pressure to obtain the total alkaloids, and use acid-dissolved The principle of alkali precipitation improves the extraction rate of the total alkaloids in daphnia.

Description

technical field [0001] The invention relates to a method for extracting total alkaloids from daguna japonica. Background technique [0002] Datura datura is the dried flower of Datura datura, a plant of the Solanaceae family. It has the effects of relieving asthma, relieving cough, relieving pain, and relieving spasm. Its main component is tropane-type alkaloids, which have strong physiological activities. The main components of withanolide steroids and flavonoids are difficult to separate during extraction. How to fully extract the active ingredients of alkaloids and improve the utilization rate is the key to improving the quality of its finished products. The extraction research of common datura alkaloids is mainly focused on immersion extraction, reflux extraction and microwave extraction (Chinese patent medicine, volume 34, issue 5, May 2012). , can also be dissolved in chloroform, ether and other organic solvents, alkaloid salts, especially inorganic acid salts, are e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07G5/00A61K36/81A61K133/00
CPCA61K36/81A61K2236/19A61K2236/35A61K2236/39
Inventor 王伟曹亮律涛何朝星王晓晖付炎薛忠杨少坤师宁宁王亚博
Owner HEBEI MEDICAL UNIVERSITY
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