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A Fluorescence Ratio Detection Method for Hypochlorite

A technology for detecting hypochlorous acid and hypochlorite, which is applied in fluorescence/phosphorescence, measuring devices, and material analysis through optical means, and can solve problems such as difficult quantification

Active Publication Date: 2020-10-27
HENAN UNIV OF ANIMAL HUSBANDRY & ECONOMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main mechanism of action of the currently reported fluorescent probe method for the detection of hypochlorite is based on fluorescence enhancement or fluorescence quenching. Difficult to quantify and other disadvantages

Method used

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  • A Fluorescence Ratio Detection Method for Hypochlorite
  • A Fluorescence Ratio Detection Method for Hypochlorite
  • A Fluorescence Ratio Detection Method for Hypochlorite

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0044] The preparation steps of the probe M1 used in this application are:

[0045] S1. In a 50 mL single-necked flask, under stirring at room temperature, add 3.0 mL of hydrazine hydrate solution dropwise to 30 mL of ethanol solution containing 1.2 g of rhodamine B hydrochloride, heat to reflux for about two hours, and detect the reaction by TLC Complete, cool to room temperature, remove the solvent under reduced pressure to obtain a red solid, add about 50 mL of dichloromethane to dissolve the solid completely, wash the system with saturated NaCl solution (10 mL) three times, dry the organic phase with anhydrous sodium sulfate, filter, and reduce The solvent was removed under pressure to obtain 1.03 g of light yellow solid product which was intermediate 1, and the yield was 90%.

[0046] S2. In a 50 mL one-necked flask, dissolve commercially purchased 4-bromo-1,8-naphthalene anhydride (413.8 mg, 1.5 mmol) and morpholine (0.2 g, 2.3 mmol) in 25 mL of ethylene glycol monomethy...

Embodiment 1

[0056] A HEPES buffer solution with a concentration of pH=7.4 and 10 mM was prepared, and a mixed solution A with a volume ratio of 4:1 was prepared with acetonitrile, and a probe M1 solution with a concentration of 1 mM was prepared with acetonitrile. The selectivity of probe M1 to hypochlorite was investigated by fluorescence spectrometer. as attached figure 1 As shown, under the excitation condition of excitation wavelength of 420 nm, the single probe M1 (10 µM) only has a strong fluorescence emission intensity at 534 nm in the mixed solution A, when adding hypochlorite (100 µM) After that, the fluorescence emission intensity at 534 nm was significantly reduced, and the fluorescence emission intensity at 580 nm was significantly enhanced, but when other analytes (100 μM) were added, the fluorescence emission intensity of the solution system was comparable to that of the single probe system. than no significant change. attached figure 2 For the above results, the fluore...

Embodiment 2

[0058] A HEPES buffer solution with a concentration of pH=7.4 and 10 mM was prepared, and a mixed solution A with a volume ratio of 4:1 was prepared with acetonitrile, and a probe M1 solution with a concentration of 1 mM was prepared with acetonitrile. The selectivity of probe M1 to hypochlorite was investigated by UV-Vis spectrometer. as attached image 3 As shown, the single probe M1 (10 µM) only has a strong UV absorption intensity at 398 nm in the mixed solution A, and when hypochlorite (100 µM) is added, the UV absorption intensity at 566 nm is significantly However, when other analytes (100 µM) were added, the UV absorption intensity of the solution system did not change significantly compared with the fluorescence emission intensity of the probe system alone. attached figure 2 For the above results, the ratio of UV absorption intensity (F 566 / F 398 ) histogram. The above experimental results show that the probe has good specificity and selectivity for hypochlorit...

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Abstract

The invention provides a method for the fluorescence ratio detection of hypochlorite ions. The method for the fluorescence ratio detection of hypochlorite ions is a method for detecting the hypochlorite ions based on a rhodamine-naphthalimide conjugate (M1) ratio. The detection method is concretely characterized in that the ratio detection of the hypochlorite ions is realized through detecting a ratio of the fluorescence emission intensities at different wavelengths (580 nm and 534 nm) in an HEPES buffer solution with the M1 as a fluorescent reagent. The detection method has a high sensitivityto the hypochlorite ions, the detection limit is as low as 1.36 * 10<-8> mol / L, and the detection method has the advantages of simple detection process, strong anti-interference ability, rapidness, sensitiveness, accurate detection result, and broad application prospect in the biomolecular detection field.

Description

technical field [0001] The present invention belongs to hypochlorite (ClO - ) detection technology field, in particular to a method for ratiometric detection of hypochlorite. Background technique [0002] Hypochlorous acid is a kind of active oxygen with strong oxidizing ability that plays an important physiological role in organisms, and plays an important role in the immune system of organisms. Under physiological conditions, hypochlorous acid can dissociate into hypochlorite to complete the important physiological protection mission of antibacterial and anti-inflammatory. Hypochlorite is closely related to our daily life. For example, the main component of common bleach and disinfectant in daily life is sodium hypochlorite. Hypochlorous acid (usually its sodium salt) is widely used in daily life as a bleaching agent, deodorant and disinfectant. As an important reactive oxygen species, hypochlorous acid plays a very important role in maintaining the redox balance in ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 马志伟陈晓培刘志景刘俊桃吴金松崔丽伟吕全建
Owner HENAN UNIV OF ANIMAL HUSBANDRY & ECONOMY
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