A Tetraploid Breeding Method of Non-heading Chinese Cabbage

A technology of head cabbage, sports, applied in the field of genetic breeding

Inactive Publication Date: 2020-04-10
南京理想农业科技有限公司
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Problems solved by technology

However, there is no report on the mixed mutagen of colchicine and APM. In this study, a mixture of colchicine, Tween 20 and APM was used to induce tetraploidy of non-heading Chinese cabbage 'Dongshangwei' in order to obtain High-efficiency and low-toxic tetraploid mutagenesis method

Method used

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  • A Tetraploid Breeding Method of Non-heading Chinese Cabbage

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Embodiment 1

[0030] 1. Materials and methods

[0031] 1.1 Materials

[0032] 'Dongshangwei' is diploid non-heading Chinese cabbage (commonly known as Chinese cabbage) (2n=2x=20), which comes from Japan (strain) Musang Wild Seeds Seedling Garden. On October 10, 2015, the seeds were sown in plug trays (50 holes) equipped with leafy vegetable substrates (Huai'an Chaimihe Agricultural Science and Technology Development Co., Ltd.). Put it into the artificial climate chamber and adjust the optical density to 200 μmol m -2 ·s -1 , the light hours are set to 12h·d -1 , the daytime temperature is set at (22±2)°C, the night temperature is set at (18±2)°C, the relative humidity is set at 60%-65%, and regular ventilation [10] . 1.2 Processing method

[0033] When the angle between cotyledon petiole is 40°-60°, when the first true leaf is not out, use a micro-injector to drip treatment on the growth point, twice a day, once at 7:30-8:30 in the morning, and at 3:30-4 in the afternoon :30 once, 20...

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Abstract

The invention discloses a tetraploid breeding method for non-heading Chinese cabbages. The method comprises the step of treating a growing point with a combined mutagenic agent when a cotyledon petiole included angle of diploid non-heading Chinese cabbages is of 40-60 degrees and a first true leaf does not grow, wherein the combined mutagenic agent is mainly composed of 0.1-0.2% colchicine and 40-60 [mu]mol / L amiprophos-methyl (APM), and treatment is performed for 2 to 4 times by using the combined mutagenic agent. According to the method disclosed by the invention, the tetraploid mutagenesisrate is as high as 12.6% by using a mixture of the low-concentration colchicine and APM, the mutagenesis rate is improved, and pollution to the environment and toxic effects on plants are reduced.

Description

technical field [0001] The invention belongs to the field of genetic breeding and relates to a tetraploid breeding method of non-heading Chinese cabbage. Background technique [0002] Polyploidy is one of the trends in plant evolution. Chemical mutagenesis is a commonly used method for artificial induction of polyploidy. Colchicine is currently the most effective mutagen, which can interfere with microtubule assembly, destroy spindle formation and terminate cells. The role of division [1] , other unfavorable mutations rarely occur in the genetic [2] . In the study of colchicine-induced doubling of non-heading Chinese cabbage, Liu Huiji [3] For the first time, 0.4% colchicine was used to treat the growth point of non-heading Chinese cabbage, and tetraploid plants were successfully obtained. Zhang Zhenchao et al [4] and Xu Lijuan [5] It is reported that 0.2% colchicine is used to treat the growth point of non-heading Chinese cabbage 4 times, the effect is the best, and t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 李艳艳周鸿章杨阳张蜀宁金业浒周金泉
Owner 南京理想农业科技有限公司
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