Antibody preservation solution and preparation method thereof

Abstract

The invention discloses an antibody preservation solution. The antibody preservation solution is prepared from 2.3-3.5 parts of sodium hydrogen phosphate, 0.23-0.35 part of sodium dihydrogen phosphate, 6-12 parts of sodium chloride, 5-15 parts of bovine serum albumin, 0.8-4.5 parts of casein sodium, 3-4.5 parts of ethylene diamine tetraacetic acid disodium, 5-15 parts of polyethylene glycol 4000,2-5 parts of glycerol, 1.5-4.5 parts of Tween-20, 0.5-1.5 parts of merthiolate sodium, 0.05-0.1 part of gentamicin and 900-1,000 parts of water; and the pH is 7.0-8.0. The invention further disclosesa preparation method of the antibody preservation solution. By means of the antibody preservation solution, the stability of various antibody molecules can be maintained, the bioactivity of antibodiescan be kept to the greatest extent, the shelf life of the antibody molecules is prolonged, and compared with a commonly-used phosphate buffer solution containing bovine serum albumin, the antibody preservation solution has obvious advantages.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an antibody preservation solution and a preparation method thereof. Background technique [0002] Antibody refers to the immunoglobulin produced by plasma cells differentiated from B lymphocytes by the body's immune system under the stimulation of antigens, and can specifically bind to corresponding antigens. Whether the antibody is properly preserved or not directly determines the activity and use effect of the antibody. For most antibodies, long-term storage at -20°C is generally required, and it is difficult to achieve long-term storage at room temperature. At the same time, repeated freezing and thawing will lead to antibody denaturation and multimer formation, thereby reducing the activity of the antibody. In most cases, antibodies can be stored at 4°C for one to two weeks, avoiding damage to antibody activity caused by repeated freezing and thawing. However, after diluting th...

AI Technical Summary

Problems solved by technology

In the process of antibody utilization, whether it is properly preserved or not directly affects the activity and use effect of the antibody. For most antibodies, it is usually stored at low temperature. Using this method of storage, repeated freezing and thawing of the antibody will lead to denaturation of the antibody, multiple Polymerization, thereby reducing antibody activity