High flux continuous flow analysis method of tobacco gene editing material sulfate content
A technology of gene editing and flow analysis, applied in the field of tobacco chemistry research, can solve the problems affecting the reliability of the test results, not being simple enough, and affecting the efficiency of the test work, and achieve the simplification of the sample pretreatment process, speed up the process, and improve the efficiency of sample analysis Effect
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Embodiment 1
[0024] Embodiment 1: Determination of sulfate in flue-cured cigarette
[0025] 1) Sample pretreatment: first dry the shredded tobacco, and use a high-speed cyclone mill to break the cell wall of tobacco and tobacco products, then pass through a 60-mesh sieve, and seal and store as a sample. Test the water content of the sample according to the YC / T296-2009 standard, and proceed as follows:
[0026] A. Accurately weigh 0.25g of sample into a 25mL improved sample tube, remove the sample tray from the automatic sampler of the continuous flow analyzer, and fill the sample tube with the weighed sample in order to inject into the continuous flow analyzer 100 sampling tube positions in the tray;
[0027] B. Use a 25mL pipette gun to add 25mL of deionized water to each sample tube, and then insert the inner casing of the filter sieve plate with a pore size of 0.45μm at the bottom in order to prevent the solution from splashing out during the vibration extraction process. Put the sam...
Embodiment 2
[0031] Embodiment 2: Determination of sulfate in mixed type cigarette sample
[0032] The process in Example 1 was repeated to measure sulfate in the mixed cigarette sample, and the sulfate content in the sample was determined to be 0.95%.
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