High flux continuous flow analysis method of tobacco gene editing material potassium content

Abstract

The invention discloses a high flux continuous flow analysis method of tobacco gene editing material potassium content. The high flux continuous flow analysis method of tobacco gene editing material potassium content comprises following steps: A, 0.25g of a potassium-containing sample is weighed accurately, and is introduced into each 25mL sample tube, a sampling disc of a continuous flow analyzerautomatic sampler is loaded with the sample tubes until full loading is realized; B, a pipette gun is used for introducing 25ml of deionized water into each sample tubes, the sample tubes are inserted into internal sleeving pipes successively to avoid solution splashing in vibration extraction process, and then the sampling disc is arranged on a oscillator for vibration extraction, wherein the bottom of each internal sleeving pipe is provided with a filter screening plate with a pore diameter of 0.45<mu>m; and C, after extraction, the internal sleeving pipes are pressed downward, so that an extracted sample solution flows into the internal sleeving pipes through the filter screening plates, the sampling place is arranged on the continuous flow analyzer automatic sampler, sampling needlesof the continuous flow analyzer automatic sampler are driven to penetrate into the internal sleeving pipes to a supernatant to absorb extracted samples, and the extracted samples are introduced into acontinuous flow analyzer for analysis.

Description

technical field [0001] The invention belongs to the field of tobacco chemistry research, and in particular relates to a high-throughput analysis method suitable for detecting potassium content in tobacco gene editing materials. Background technique [0002] With the advancement of the tobacco industry's "Tobacco Genome Project Major Project-Based Genome Editing Technology Factory Breeding Program", all gene-edited materials will be screened through chemical composition analysis, and the compatibility test of tobacco leaves and cigarette formula will be passed. Verification, to determine the varieties with strong industrial usability, has become a key technical content urgently needed by the tobacco industry. [0003] Potassium is an important conventional chemical component in tobacco and one of the factors that affect the important quality of tobacco leaves. It helps to improve the combustibility of tobacco leaves, and enhances the safety, aroma value and aroma volume of to...

AI Technical Summary

Problems solved by technology

During the sample pretreatment process, the sample needs to be transferred several times, which is not convenient enough and affects the efficiency of the detection; and the reliability of the detection results may be affected by the introduction of impurities and the careless spilling of the extract during the transfer process.

[0005] Factory breeding for gene editing will produce at least tens of thousands of editing materials. At present, the daily sample processing capacity of potassium sample analysis is only about 60. It takes a long period to complete the routine chemical composition analysis of tens of thousands of materials, and the lag of analysis results will affect Therefore, there is an urgent need for accurate and reliable high-throughput rapid analysis methods in the process of gene editing breeding

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