Device for pretreatment of multiple samples and application method of device

Abstract

The invention discloses a device for pretreatment of multiple samples. The device comprises the components of a sample extraction tube (3), a plugged bottom plate (9), a sample extraction frame (2), asieve plate cover (4), a fixed cover plate (5), a feeding disk (1), a pushrod flat plate (10) and an oscillator (12). The invention further discloses an application method of the device. By use of the device, sample analysis efficiency can be improved by multiple times.

Description

technical field [0001] The invention belongs to the field of tobacco chemistry research, and in particular relates to a device for pretreatment of multiple samples and an application method thereof. Background technique [0002] With the advancement of the tobacco industry's "Tobacco Genome Project Major Project-Based Genome Editing Technology Factory Breeding Program", all gene-edited materials will be screened through chemical composition analysis, and the compatibility test of tobacco leaves and cigarette formula will be passed. Verification, to determine the varieties with strong industrial usability, has become a key technical content urgently needed by the tobacco industry. [0003] The continuous flow analysis technology pumps the standard working fluid, samples and reagents into the chemical analyzer in proportion, and at the same time sends the gas regularly, so that a continuous fluid separation system is formed in the pipeline, so that the substances participating...

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Problems solved by technology

[0004] There are many deficiencies in the traditional continuous flow analysis test sample pretreatment: ①The traditional sample weighing process is heavy, tedious and time-consuming, which seriously affects the progress of the test work; Measuring instruments are labor-intensive and time-consuming, and unskilled operators may easily lead to inconsistent volumes of the extractant, which will affect the test results; if the reagent bottle is equipped with a quantitative device and the hand pressure method is used to take the liquid, it is easy to produce air bubbles , resulting in an inaccurate pipetting volume of the extractant, which will also affect the test results; ③In the pretreatment, the sample needs to be weighed into a ground-mouth Erlenmeyer flask, the extractant is added to vibrate for extraction, filtered with qualitative filter paper, and the filtrate is transferred to a continuous flow into the sampling line of the analyzer before subsequent determinations can be made

During the sample pretreatment process, the sample needs to be transferred several times, which is not convenient enough and affects the efficiency of the detection; and the reliability of the detection results may be affected by the introduction of impurities and the careless spilling of the extract during the transfer process.

[0005] Factory breeding of gene editing will produce at least tens of thousands of editing materials. At present, the daily sample processing capacity of conventional chemical composition analysis is only about 60. It takes a long time to complete the routine chemical composition analysis of tens of thousands of materials, and the lag of analysis results will be huge. Affects the progress of the entire factory breeding, so there is an urgent need for accurate and reliable high-throughput rapid analysis methods in the process of gene editing breeding

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