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Extracellular polymeric substance and preparation method thereof, heavy metal treating agent and heavy metal waste water treating method

An extracellular polymer, heavy metal technology, applied in water/sewage treatment, adsorbed water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problem of poor heavy metal removal ability, achieve excellent adsorption effect, and be environmentally friendly , the effect of simple operation

Active Publication Date: 2018-09-07
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides an extracellular polymer and its preparation method, a heavy metal treatment agent and a heavy metal wastewater treatment method, and solves the technical problem that the existing microbial extracellular polymer has poor ability to remove heavy metals

Method used

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  • Extracellular polymeric substance and preparation method thereof, heavy metal treating agent and heavy metal waste water treating method
  • Extracellular polymeric substance and preparation method thereof, heavy metal treating agent and heavy metal waste water treating method
  • Extracellular polymeric substance and preparation method thereof, heavy metal treating agent and heavy metal waste water treating method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Exogenous Sulfur-Induced Extracellular Polymers

[0045] In this example, Bacillus vallismortis sp. (Bacillus vallismortis sp., belonging to aerobic heterotrophic bacteria, obtained by separation and purification in a laboratory) was selected. The specific preparation steps of the extracellular polymer are as follows:

[0046]The Bacillus dead valley stored at -18°C was taken out, thawed to room temperature, activated and cultured, and shaken at a constant temperature for 48 hours to prepare a Bacillus dead valley suspension. During induction culture, inoculate the bacterial suspension into the liquid culture medium that has been autoclaved at 121°C for 20 minutes under aseptic conditions according to 5% inoculum amount (the culture medium is packed in conical flasks, each conical flask Containing 100mL culture medium), shake at constant temperature in a shaker for 2 hours, and then inject sterilized sodium sulfide solution into the liquid medium under asepti...

Embodiment 2

[0047] Example 2 Determination of main components of extracellular polymer

[0048] In the determination of the main components of extracellular polymers, the following methods were used: the anthrone colorimetric method was used to determine the total sugar content, the Coomassie Brilliant Blue G-250 method was used to determine the protein content, and the diphenylamine method was used to determine the nucleic acid content. Total sugars, proteins, and nucleic acids together characterize the composition of extracellular polymers.

[0049] Depend on figure 1 and image 3 It can be seen that, in the embodiments of the present invention, compared with before induction, Bacillus death valley, at different concentrations of S 2- Under induction, among the specific extracellular polymers produced by Bacillus death valley, the protein content was the highest when the concentration of sodium sulfide was 40 mg / L and sodium thiosulfate concentration was 60 mg / L, while the trend of to...

Embodiment 3

[0050] Example 3 extracellular polymer to heavy metal Cu 2+ Determination of adsorption performance

[0051] Measure the specific extracellular polymer 2mL produced under the exogenous sulfur induction of Bacillus death valley in Example 1 and add to 20mL, Cu 2+ In wastewater with a concentration of 20mg / L, adjust the pH to about 5.0, shake at 150rpm for 2h, then put the mixture into a dialysis bag with a molecular weight cut-off of 4000, dialyze with deionized water for 12h, and measure the dialysis with a flame atomic absorption spectrophotometer. after Cu 2+ concentration, a blank test was performed with uninduced extracellular polymeric substances. Three parallel samples were set up for each sample, and the average value of the experimental data was taken.

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Abstract

The invention relates to the technical field of environment microorganism and waste water treatment, in particular to an extracellular polymeric substance and a preparation method thereof, a heavy metal treating agent and a heavy metal waste water treating method. The preparation method for the extracellular polymeric substance comprises the following steps that exogenous sulfur induction treatment is carried out on an aerobic heterotrophic bacterium to obtain the extracellular polymeric substance; the exogenous sulfur is one or more of sodium sulfide, sodium sulfite, sodium thiosulfate and sodium hydrosulfite. The preparation method is operated simply, and environmentally-friendly, it can be known through the test data that after the exogenous sulfur induction is carried out, the composing proportion of the extracellular polymeric substance changes correspondingly, the adsorption quantity for cuprum<2+> and zinc<2+> of the extracellular polymeric substance nearly reaches 1 gram per gram or above, the excellent adsorption effect is shown, and the technical problem that the poor removal capacity of the microorganism extracellular polymeric substance for the heavy metal is solved.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms and waste water treatment, in particular to an extracellular polymer and a preparation method thereof, a heavy metal treatment agent and a heavy metal waste water treatment method. Background technique [0002] Heavy metal pollution refers to environmental pollution caused by heavy metals or their compounds. Heavy metal pollution in water mainly comes from human social activities, including mining and metallurgy, metal processing, chemical industry, electroplating, use of pesticides and fertilizers, pollution accidents, etc. Concentration and chemical form present in food and organisms. Heavy metals are a class of pollutants that are extremely harmful to the ecological environment. They cannot be biodegraded after entering the environment. They often participate in the food chain cycle and eventually accumulate in organisms, destroying the normal physiological and metabolic a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04C02F1/28C02F101/20C02F101/22
CPCC02F1/286C02F2101/20C02F2101/22C12P1/04
Inventor 宋卫锋李秋华杨梓亨李家耀杜璞欣
Owner GUANGDONG UNIV OF TECH
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