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Prostate cancer detection probe sequence and application thereof

A prostate cancer and sequence technology, which is applied in the field of prostate cancer detection probe sequences, can solve problems such as the inability to guarantee specificity, the influence of quantification limit and detection limit sensitivity, etc.

Active Publication Date: 2018-09-07
ZHENGZHOU KODIA BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, different QuantiMAT probe sequences have the most direct impact on the specificity of various cancers such as prostate cancer and cervical cancer. The QuantiMAT probe sequences for PCA3 designed according to the ProbeDesigner V1.0 tool cannot detect prostate cancer. Guaranteed high specificity at the expense of limits of quantitation (LOQ), limits of detection (LOD), and sensitivity

Method used

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  • Prostate cancer detection probe sequence and application thereof
  • Prostate cancer detection probe sequence and application thereof
  • Prostate cancer detection probe sequence and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: The kit for detecting prostate cancer of the present invention

[0046] 1. Probe sequence

[0047] The capture extension probe sequence is shown in SEQ ID No. 1-14 and SEQ ID No. 78-85, and the K-type labeled extension probe is shown in SEQ ID No. 15-64 and SEQ ID No. 86-131, closed The probes are shown in SEQ ID No. 65-77 and SEQ ID No. 132-148;

[0048] 2. Kit components

[0049] Universal probes coupled to carriers such as magnetic beads or cell culture plates: shown in SEQIDNo.149;

[0050] Signal amplification precursor: ATGCTTTGACTCAG / AAAACGGTAACTTC (amplified precursor pre-leader sequence, two kinds) + AGGATCGTAAGTAACTAAGGACTACC (amplifier pre-leader complementary sequence) + 19 times amplifier pre-leader complementary sequence (SEQ ID No. 150-151 Shown);

[0051] Signal amplifier: GGTAGTCCTTAGTTACTTACGATCCT (amplifier front guide region sequence) + GAATCCATTGAATCCTGTGATT (reporter probe repeat region complementary sequence) + 19 times reporter probe repeat reg...

Embodiment 2

[0054] Example 2: Specificity comparison test

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Abstract

The invention relates to the technical field of genetic engineering and discloses a prostate cancer detection probe sequence and the application thereof. The probe sequence comprises a capturing extension probe, a K-type marking extension probe and a sealing probe. The capturing extension probe, the K-type marking extension probe and the sealing probe are designed and artificially optimized for prostate cancer PCA3, the specificity of prostate cancer cell detection based on similar bDNA and QuantiMAT techniques can be improved, relatively good quantitative limits, detection limits and sensitivity can be achieved, and the probe can be applied to preparation of kits.

Description

Technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a prostate cancer detection probe sequence and its application. Background technique [0002] PCA3 is the new prostate cancer antigen 3. PCA3 was discovered in 1995 by Johns Hopkins Hospital (Baltimore) and Radboud University (Nijmegen, Netherlands). It was originally named DD3 (differential display clone, 3) because of the use of differential display analysis (in that Time is a new description method used to identify gene expression in different tissues) Compare the mRNA expression pattern of normal vs cancerous prostate tissue, and find that a kind of mRNA is highly specifically expressed in prostate cancer. [0003] PCA3 is overexpressed in 95% of all prostate cancers detected, and can be measured harmlessly from cancerous prostate cells with an accuracy close to 100%. In addition, PCA3 transcription has not been detected in extraprostatic tissues (benign and malignant),...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/682C12N15/11
CPCC12Q1/682C12Q1/6886C12Q2565/519
Inventor 张鹭鹭张惠箴黄谨李先坤田小强徐文茹李鑫李小磊孟歌何丽韩燃郭曙光苏芹
Owner ZHENGZHOU KODIA BIOTECHNOLOGY CO LTD
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