A kind of antibiotic degrading mixed bacteria agent and its application
A technology of mixing bacterial agents and antibiotics, applied in the directions of bacteria, fungi, microorganisms, etc., can solve the problem of few antibiotics degrading mixed bacterial agents, and achieve the effects of clear bacterial composition, high degradation efficiency, and good application prospects.
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[0039] One, the domestication, screening and identification method of Arthrobacter sp. and Alcaligenes sp., comprising the following steps:
[0040] 1) Take about 5g of sludge and add them to the oligotrophic enrichment medium and nutrient enrichment medium containing target antibiotics (1, oxytetracycline, 50mg / L; 2, sulfadimethoxine, 50mg / L) respectively , placed in a constant temperature shaker (30° C., 150-180 rpm) and incubated in the dark for one week.
[0041] 2) After the culture of the previous stage was allowed to stand, 5 ml of the supernatant was taken and transferred to the same resistance-enriched medium. After culturing for 7 days, the residual concentration of antibiotics was measured. A total of 3 cycles of enrichment were performed.
[0042] 3) Compare the antibiotic residue concentrations in the oligotrophic enrichment culture and the eutrophic enrichment culture, select the enrichment culture with a higher antibiotic degradation rate, and after standing fo...
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