Hericium erinaceus strain and breeding method thereof

A technology of Hericium erinaceus and strains, applied in botany equipment and methods, horticulture, plant cultivation, etc., can solve problems such as degradation, unstable cultivation environment, etc.

Active Publication Date: 2018-09-28
SHANGHAI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

With the in-depth research on Hericium erinaceus and the development of Hericium erinaceus products, the demand for high-yield polysaccharide Hericium erinaceus has increased sharply, and the existing Hericium erinaceus polysaccharides mainly come from fruiting bodies and solid fermentation mycelia cultivated in traditional models Due to the instability of the cultivation environment and the degradation of the strain, the polysaccharide content is not high and unstable, so it is particularly important to obtain high-quality Hericium erinaceus

Method used

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  • Hericium erinaceus strain and breeding method thereof
  • Hericium erinaceus strain and breeding method thereof

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Embodiment 1

[0027] (1) Preparation of protoplasts and selection of monokaryons

[0028] (1) Activation and cultivation of the parental strains: transfer the slant strain 0605 and spines to PDA plates, and culture them in an incubator at 26°C for 10-15 days. Pick the plate mycelium into a homogenizer, add a small amount of liquid culture medium, homogenize and crush it, inoculate it in 100mL liquid culture medium, keep the temperature at 26°C, and culture it statically for 10 days, during which the mycelia are slightly shaken 2-3 times a day.

[0029] (2) Preparation of protoplasts: the hyphae were collected by filtration and cultured statically, rinsed with sterile water for 1-2 times, and the water on the surface of the mycelia was blotted dry with sterile absorbent paper. According to every 0.3g mycelia, add 1mL enzyme solution (0.2g wall-lyzing enzyme (purchased from Guangdong Institute of Microbiology), 0.05g disintegrating enzyme (purchased from Sigma) and dissolve in 10mL 0.6mol / L m...

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Abstract

The invention provides a hericium erinaceus strain. A good strain ZJ611 which has high mycelium biomass, stable per unit area yield and high fruiting body polysaccharide content and has the collectionnumber of CGMCC No.14990 is obtained through preparation of parental strain protoplast, selection of mononuclear bodies, identification of hybrid strains, further screening of the hybrid strains andscreening of hybrid strain cultivation fruiting bodies. The growth rate of the hericium erinaceus strain reaches 0.308 cm / d, which is 12% higher than that of parent strains; the shake flask biomass reaches 12.23 g / L, which is 43.07% higher than that of parent strains; the intracellular polysaccharide yield is 0.89 g / L, which is 36% higher than that of parent strains; the per unit area yield of cultivation fruiting bodies reach 87 g / bag; the polysaccharide content reaches 8.54%, which is 63.9% higher than that of parents.

Description

technical field [0001] The invention belongs to the field of microbial cross-breeding, and in particular relates to a Hericium erinaceus strain and a breeding method thereof. Background technique [0002] Hericium erinaceus is a well-known edible and medicinal fungus in my country, which belongs to Basidiomycota, Hericium erinaceae, and Hericium erinaceus. Hericium erinaceus has the functions of protecting liver and stomach, lowering blood sugar, protecting nerves, enhancing human immunity, anti-cancer, anti-oxidation, etc. It can treat neurasthenia, gastritis and gastric ulcer, etc. It has high edible and medicinal value. Developed as related pharmaceutical preparations. [0003] The chemical composition of Hericium erinaceus is very complex, including polysaccharides, sterols, terpenes, fatty acids, phenols and other compounds, among which the polysaccharides of Hericium erinaceus are the most important active ingredients, which have the functions of improving immunity, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20A01G18/40A01G18/50A23L33/125
CPCA01G18/00A01G18/20A01G18/40A01G18/50A23L33/125
Inventor 杨焱杨珊张赫男李巧珍吴迪李正鹏陈明杰张劲松刘艳芳汪雯翰唐传红周帅张忠
Owner SHANGHAI ACAD OF AGRI SCI
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