Hericium erinaceus strain and breeding method thereof
A technology of Hericium erinaceus and strains, applied in botany equipment and methods, horticulture, plant cultivation, etc., can solve problems such as degradation, unstable cultivation environment, etc.
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[0027] (1) Preparation of protoplasts and selection of monokaryons
[0028] (1) Activation and cultivation of the parental strains: transfer the slant strain 0605 and spines to PDA plates, and culture them in an incubator at 26°C for 10-15 days. Pick the plate mycelium into a homogenizer, add a small amount of liquid culture medium, homogenize and crush it, inoculate it in 100mL liquid culture medium, keep the temperature at 26°C, and culture it statically for 10 days, during which the mycelia are slightly shaken 2-3 times a day.
[0029] (2) Preparation of protoplasts: the hyphae were collected by filtration and cultured statically, rinsed with sterile water for 1-2 times, and the water on the surface of the mycelia was blotted dry with sterile absorbent paper. According to every 0.3g mycelia, add 1mL enzyme solution (0.2g wall-lyzing enzyme (purchased from Guangdong Institute of Microbiology), 0.05g disintegrating enzyme (purchased from Sigma) and dissolve in 10mL 0.6mol / L m...
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