New phenolic acid compound, preparation method and medical application thereof

A technology of phenolic acids and compounds, which is applied in the field of preparation of phenolic acids and can solve problems such as few

Inactive Publication Date: 2018-10-02
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that celery has good antibacterial activity, but there are not many basic research and reports on its antibacterial active substances

Method used

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  • New phenolic acid compound, preparation method and medical application thereof
  • New phenolic acid compound, preparation method and medical application thereof
  • New phenolic acid compound, preparation method and medical application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) 1000g of the dried whole herb of Feicai, crushed, heated and ultrasonically extracted with 70% ethanol once (amount of 8L), and recovered the crude extract of the extract under reduced pressure;

[0028] (2) The 70% ethanol crude extract obtained in step (1) is adsorbed by a macroporous resin, and gradient eluted with water, 60% and 90% ethanol-water mixed solvent;

[0029] (3) The 60% ethanol eluate obtained in step (2) is separated by silica gel column chromatography, and is separated by ethyl acetate / methanol 100:1, 100:2, 100:5, 100:8, 100:10, 4:1, 2:1, 1:1 mixed solvent gradient elution;

[0030] (4) step (3) ethyl acetate / methanol 100:3 fraction, carry out high-efficiency liquid phase separation, 250nm detection, flow velocity is 1mL / min, mobile phase is methanol: water=60:40, obtainsedumol A(t R = 7.9 min).

[0031] According to the physical and chemical properties and spectral data of sedumol A, its structure was identified (see the attached NMR spectrum a...

Embodiment 2

[0040] (1) 2000 g of the dried whole herb of Feicai, crushed and extracted twice under reflux with 40% ethanol (amount of 20 L), and the crude extract of the extract was recovered under reduced pressure;

[0041] (2) The 40% ethanol crude extract obtained in step (1) is adsorbed by a macroporous resin, and eluted with water, 10%, 50% and 90% ethanol-water mixed solvent;

[0042] (3) 50% ethanol eluate in step (2), followed by dichloromethane / methanol mixed solvent 100:1, 100:2, 100:3, 100:8, 100:10, 4:1, 2: 1, 1:1 gradient elution;

[0043] (4) The dichloromethane / methanol 100:8 fraction obtained in step (3) adopts high performance liquid phase separation, and 250nm detects, and flow rate is 1mL / min, and mobile phase is methanol: water=60:40, obtains sedumol A ( tR = 7.9 min).

Embodiment 3

[0045] (1) Preparation method of bacterial suspension

[0046] Inoculate Escherichia coli, Staphylococcus aureus, and Bacillus subtilis into the broth and culture at 37°C for 18-24 hours. Add 10ml of sterile distilled water to the sterile test tubes respectively, draw the bacterial solution into the test tubes with a pipette gun, and shake fully for 30 minutes to make a bacterial suspension. Count under a microscope with a hemocytometer. Adjust the bacterial concentration to l05CFU / ml with liquid medium.

[0047] (2) Detection method of minimum inhibitory concentration (MIC) by liquid trace method

[0048]For aseptic operation, add 100 μL of the corresponding liquid medium for detection to each small well of a sterilized 96-well polystyrene culture plate, add 100 μL of the sample to be tested to the first small well, and use a pipette gun to Inhale repeatedly and mix well. Take 100 μL from the first small hole and put it into the second small hole, mix, perform 2-fold prop...

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Abstract

The invention relates to a structure and preparation method of a new phenolic acid compound and application in the antibacterial field. According to the invention, extraction of the sedum aizoon overground part, separation and purification are carried out to obtain a new compound named as sedumol A. Pharmacodynamic experiments show that sedumol A has strong inhibitory effect on Escherichia coli, Staphylococcus aureus and the like.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a preparation method and medical application of a new phenolic acid component in celery. Background technique [0002] Sedum aizoon L. is a plant of the genus Sedum in the Crassulaceae family. It has a long history of medicinal use in the folk and is widely used to prevent and treat cardiovascular diseases. There are about 470 species of plants in the genus Sedum, which are mainly distributed in the northern hemisphere. There are 124 species, 1 subspecies, 14 variants and 1 variant in China, mainly distributed in the southwest region. The sedum plant has a long history of medicinal use among the people in our country. There are many kinds of medicinal plants in this genus, which have a wide range of effects, such as clearing away heat and detoxification, stopping bleeding and astringent, and treating sores, carbuncles and furuncles. [0003] Feicai is also known as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/84C07C67/48C07C67/56A61P31/04
CPCA61P31/04C07C69/84Y02A50/30
Inventor 路宜蕾吕重宁
Owner SHANDONG UNIV
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