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Compound microbial purifying agent for river sewage purification and preparation method thereof

A composite microorganism and sewage purification technology, which is applied in the field of composite microorganism purifying agent for river sewage purification and its preparation, can solve the problems of complex preparation, high operating cost and insignificant effect, and achieves simple preparation method, economical cost and obvious effect. Effect

Active Publication Date: 2018-10-12
万创青绿(北京)环境科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a compound microbial purifying agent for purifying river sewage and its preparation method and its preparation method, so as to solve the problems of complex preparation, high operating cost and ineffective effect of the sewage purifying agent in the prior art

Method used

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preparation example Construction

[0024] The preparation method of above-mentioned compound microbial cleaning agent comprises:

[0025] A. Inoculate different high-purity strains in liquid medium, including Bacillus subtilis B.subtilis (Bacillus subtilis), photosynthetic bacteria-Rhodoopseudomonas palustris, Pseudomonas stutzeri, alternate nitrification The genus Alteromonas denitrificans and the genus Nitrosomonas europea, the number of inoculum was 1.5×10 9 ~3.5×10 9 cfu / mL, the number of inoculated bacteria is 2×10 9 cfu / mL, mixed culture and enrichment, based on this to make composite bacteria, for later use;

[0026] The components of the liquid medium include: tryptone 8-12g / L, yeast extract 3-6g / L and NaCl 8-12g / L; preferably, the components of the liquid medium include: tryptone 10g / L , yeast extract 5g / L, NaCl 10g / L; the conditions for cultivating strains are: temperature is 22°C-28°C, pH is 6.7-7.3; Soil, trace element nutrient solution, straw slag powder, corn flour and fish meal are put into a s...

Embodiment 1

[0030] Inoculate different high-purity strains in liquid medium, including Bacillus subtilis (Bacillus subtilis), photosynthetic bacteria-Rhodoopseudomonas palustris, Pseudomonas stutzeri, nitrifying alternation The genus Alteromonas denitrificans and the genus Nitrosomonas europea, the number of inoculated bacteria is 2×10 9 cfu / mL, mixed culture and enrichment, based on this to make composite bacteria, for later use; put 340kg composite bacteria, 500kg diatomaceous earth, 10kg trace element nutrient solution, 50kg straw slag powder, 50kg corn flour and 50kg fish meal into Stir in a stirring device to obtain a mixture, wherein every kilogram of trace element nutrient solution contains 0.5g of ZnSO 4 , 2g of CaCl 2 , 2.5g of MnCl 2 4H 2 O, 0.5g of (NH 4 ) 6 Mo 7 o 24 4H 2 O, 0.1g of CuSO 4 ·5H 2 O and 0.5 g CoCl 2 ·6H 2 O, 3.8g of FeCl 2 4H 2 O, dry the mixture evenly stirred at a low temperature of 35°C to obtain a composite microbial purifier in the form of sol...

Embodiment 2

[0035] Inoculate different high-purity strains in liquid medium, including Bacillus subtilis (Bacillus subtilis), photosynthetic bacteria-Rhodoopseudomonas palustris, Pseudomonas stutzeri, nitrifying alternation The genus Alteromonas denitrificans and the genus Nitrosomonas europea, the number of inoculated bacteria is 3.5×10 9 cfu / mL, mixed culture and enrichment, based on this to make composite bacteria, for later use; put 270kg composite bacteria, 550kg diatomaceous earth, 20kg trace element nutrient solution, 80kg straw residue powder, 30kg corn flour and 80kg fish meal into Stir in a stirring device to obtain a mixture, wherein every kilogram of trace element nutrient solution contains 0.3g of ZnSO 4 , 3g of CaCl 2 , 2g of MnCl 2 4H 2 O, 0.8g of (NH 4 ) 6 Mo 7 o 24 4H 2 O, 0.2g of CuSO 4 ·5H 2 O and 0.3 g CoCl 2 ·6H 2 O, 4.5g of FeCl 2 4H 2 O, dry the mixture evenly stirred at a low temperature of 35°C to obtain a composite microbial purifier in the form of ...

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Abstract

The invention relates to a compound microbial purifying agent for river sewage purification and a preparation method thereof. The compound microbial purifying agent contains compound bacteria, and thecompound bacteria include bacillus subtilis, photosynthetic bacteria-rhodopseudomonas palustris, pseudomonas stutzeri, alteromonas denitrificans and nitrosomonas europaea. The preparation method of the compound microbial purifying agent for river sewage purification is simple; the compound microbial purifying agent can quickly, comprehensively and effectively purify a water body, quickly and effectively reduce the COD, N, TP and chroma in river sewage and obviously relieve the black color and bad odor of the water body, and has obvious effects and significant ecological benefits; the compoundmicrobial purifying agent is also suitable for in-situ remediation of polluted rivers, and has the advantages of being low in cost, easy to use and the like.

Description

technical field [0001] The invention relates to the technical field of environmental protection, in particular to a compound microbial purifying agent for purifying river sewage and a preparation method thereof. Background technique [0002] In recent decades, my country's urbanization process has developed rapidly, and with the rapid development of industrialization, people's demand for water resources has increased, and river water pollution incidents have occurred frequently, and the number of black and odorous water bodies in urban built-up areas Unabated, the supply of water resources has become more tense. Among them, the problem of eutrophication of water body due to excessive discharge of organic matter, nitrogen and phosphorus is particularly prominent. [0003] As an important decomposer in the aquatic ecosystem, microorganisms play an important role in the self-purification process of river water bodies. They can quickly and effectively degrade organic matter, nit...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/125C12R1/38
CPCC02F3/34C02F2103/007C12N1/20
Inventor 张盛斌杨伟明
Owner 万创青绿(北京)环境科技有限公司
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