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A kind of new bird's nest extract and its preparation method and application

The technology of extracting liquid and bird's nest is applied to the new bird's nest extracting liquid and its preparation and application fields, which can solve the problems of low macromolecular protein bioavailability, low product protein yield, complicated process, etc., and achieve unique biological functions, The effect of simple preparation process and huge market potential

Active Publication Date: 2021-11-12
SHENZHEN MANNAY COSMETIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are many bird’s nest products on the market, most of which are extracted by steaming in a relatively simple and rough way, and usually retain almost complete macromolecular forms, but the bioavailability of macromolecular proteins is generally relatively low
Some use ultrasonic crushing of bird’s nest, and then add enzymatic hydrolysis; in addition to enzymatic hydrolysis steps, the existing technology also needs to go through pretreatment steps such as crushing, soaking, boiling, and pH adjustment to improve the efficiency of hydrolysis. The process is complicated and the product protein low yield

Method used

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  • A kind of new bird's nest extract and its preparation method and application
  • A kind of new bird's nest extract and its preparation method and application
  • A kind of new bird's nest extract and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The preparation method of the bird's nest extract described in the present embodiment comprises the following steps:

[0048] a) Soak the bird’s nest in water 30-100 times the weight of the bird’s nest, soak at room temperature for 1.5-12 hours, and filter to obtain the bird’s nest filter residue; the bird’s nest is the bird’s nest of white swallow or yellow swallow;

[0049] Stew the bird's nest filter residue with water 30-100 times the weight of the bird's nest for 3-24 hours to obtain a preliminary bird's nest extract (second-generation bird's nest extract), freeze-dry the extract to obtain the bird's nest extract and freeze dry powder;

[0050] or

[0051] When the bird’s nest is blood bird’s nest, soak the bird’s nest in water 30-100 times the weight of the bird’s nest, soak at room temperature for 12-26 hours, and filter to obtain the bird’s nest filter residue;

[0052] Stew the bird's nest filter residue with water 30-100 times the weight of the bird's nest f...

Embodiment 2

[0056] The preparation method of the bird's nest extract described in the present embodiment uses high performance liquid chromatography to detect the digestion efficiency of the bird's nest extract, comprising the following steps:

[0057] a) After the bird's nest extract obtained according to the preparation method described in Example 1 is neutralized, it is filtered with a hydrophilic 0.45 μm filter membrane.

[0058] b) High performance liquid chromatography separation

[0059] 1. Instrument: HPLC column: Superdex 75 10 / 300GL (GE Life Sciences)

[0060] 2. Chromatography conditions: flow rate 0.5mL / min, injection volume 10μL

[0061] 3. Chromatography time: 70 minutes

[0062] 4. Mobile phase: Phosphate buffered saline

[0063] 5.DAD absorption brightness: 280nm

[0064] c) Measurement results

[0065] The size exclusion chromatography of white bird's nest, yellow bird's nest and red bird's nest extracts collected at different times, the molecular weight of which is ...

Embodiment 3

[0070] The preparation method of the bird's nest extract described in the present embodiment uses high performance liquid chromatography-mass spectrometry to detect the content of free sialic acid in the bird's nest extract, comprising the following steps:

[0071] a) After neutralizing the bird's nest digestive juice obtained according to the preparation method described in Example 1, dilute it 40 times with 0.1% formic acid, and add an internal standard.

[0072] b) Centrifuge at high speed for 5 minutes, take the supernatant and inject.

[0073] c) Preparation of standard solution: Accurately measure 10mg of N-acetyl-D-neuraminic acid, place it in a 10mL volumetric flask, add water to dilute to the mark, shake well to get 1mg / ml N-acetyl-D-neuraminic acid standard solution.

[0074] d) liquid chromatography

[0075] The conditions are as follows:

[0076] 1. Instrument: HPLC column: Eclipse XDB-C18 (2.1×100mm, 3.5μm) chromatographic column

[0077] 2. Chromatography con...

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Abstract

The invention discloses the third-generation bird's nest extract and its preparation method and application. The preparation method is digested with simulated gastric acid for 1-72 hours, and can decompose the protein components greater than 100kDa in the second-generation bird's nest water extract to obtain more and more A polypeptide component that is easily absorbed by the human body, the molecular weight of the polypeptide in the bird’s nest extract is mainly distributed below 6.5kDa; at the same time, this extraction process can promote the release of free sialic acid of the bird’s nest active ingredient, and the release rate is 0.2% of the weight of the bird’s nest. ‑3.8% to 8‑12%. The bird's nest extract prepared by the method has a higher whitening ability than the first and second generation extracts, while retaining its antioxidant effect. The bird's nest extract prepared by the extraction method can be processed into various products, which are widely used in the preparation of health products, food and beauty products for whitening, anti-oxidation, anti-fatigue, anti-aging, anti-virus, and improving body immunity.

Description

technical field [0001] The present invention relates to a new bird's nest extract and its preparation method and application, especially the preparation method can obtain bird's nest extract with bird's nest polypeptide and high-concentration free sialic acid, and the bird's nest extract can be used for health care products, food and in beauty products. Background technique [0002] The bird's nest is a bird of the Swift family Swiftlet. The secreted saliva or saliva mixed with its down feathers condenses on the cliffs and cliffs to form a nest. The main production areas are Indonesia, Malaysia, Vietnam, Thailand, etc. According to the "Supplements to Materia Medica": "Bird's nest is sweet and flat, nourishes lung yin, reduces phlegm and relieves cough, nourishes and clears, and is a holy medicine for recuperating fatigue. All diseases are due to lung deficiency , and those who cannot clear up the descending, can be cured with this." Chinese medicine believes that bird's ne...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/98A23L33/10A23L2/38A61Q19/02A61Q19/08
CPCA61Q19/02A61Q19/08A23L2/38A23L33/10A61K8/981
Inventor 詹华强董婷霞黄振辉陈嘉伦谭天乐
Owner SHENZHEN MANNAY COSMETIC
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