A special diluent for alkaline phosphatase antigen antibody

An antigen-antibody and phosphatase technology, applied in the field of medicine, can solve the problems of low stability, technical difficulties, endangering the accuracy of test results, etc., and achieve the effects of reduced production cost, simple components and good hydrophilicity

Active Publication Date: 2021-01-29
南京斯博伏特新材料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the insufficient preparation process of quality control products, the stability of quality control samples used for reagent assessment, indoor quality control and external quality assessment in China is not high. The results of quality control and quality evaluation have large deviations. On the one hand, it affects the correct judgment of laboratory test results, and even directly endangers the accuracy of test results. Risk of administrative confusion and technical difficulties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A special diluent for alkaline phosphatase antigen antibody, including the following components by mass fraction: 92% phosphate buffer solution, 1% polymer hydrophilic compound, 0.2% sodium edetate, and 0.01% theophylline , modified amino acid 1%, anticoagulant 0.02%, silk protein 0.05%, nano silver powder 5.72%.

[0018] The above-mentioned phosphate buffer solution includes the following components by weight: 10 parts of sodium chloride, 2 parts of potassium chloride, 25 parts of potassium phosphate, and 100 parts of deionized water. The polymer hydrophilic compound is formed by condensation of hexane oxide, chitosan and polyethylene glycol, and the molar ratio of hexane oxide, chitosan and polyethylene glycol is 2:1:3.

[0019] Wherein, the preparation method of the modified amino acid is as follows: after dissolving the dopamine solution, adding the amino acid and stirring evenly, then adding titanium dioxide powder, continuing to stir, and spray-drying at 50° C. to...

Embodiment 2

[0023] A special diluent for alkaline phosphatase antigen antibody, including the following components by mass fraction: 93% phosphate buffer solution, 1.5% polymer hydrophilic compound, 0.3% sodium edetate, and 0.015% sodium azide %, modified amino acid 2%, anticoagulant 0.03%, silk protein 0.1%, nano silver powder 3.055%.

[0024] The phosphate buffer solution comprises the following components by weight: 12 parts of sodium chloride, 3 parts of potassium chloride, 45 parts of potassium phosphate, and 180 parts of deionized water.

[0025] Wherein, the polymer hydrophilic compound is formed by condensation of hexane oxide, chitosan and polyethylene glycol, and the molar ratio of hexane oxide, chitosan and polyethylene glycol is 3:1:4.

[0026] The preparation method of the modified amino acid is as follows: after dissolving the dopamine solution, adding the amino acid and stirring evenly, then adding titanium dioxide powder, continuing stirring, and spray drying at 60° C. to ...

Embodiment 3

[0030] A special diluent for alkaline phosphatase antigen antibody, including the following components by mass fraction: 93% phosphate buffer saline, 1.9% polymer hydrophilic compound, 0.4% sodium edetate, citronella extract 0.02%, modified amino acid 1.9%, anticoagulant 0.03%, silk protein 0.07%, nano silver powder 2.68%.

[0031] The phosphate buffer solution comprises the following components by weight: 16 parts of sodium chloride, 5 parts of potassium chloride, 50 parts of potassium phosphate, and 200 parts of deionized water.

[0032] The polymer hydrophilic compound is formed by condensation of hexane oxide, chitosan and polyethylene glycol, and the molar ratio of hexane oxide, chitosan and polyethylene glycol is 4:2:5.

[0033] Wherein, the preparation method of the modified amino acid is as follows: after dissolving the dopamine solution, adding the amino acid and stirring evenly, then adding titanium dioxide powder, continuing stirring, and spray-drying at 65° C. to o...

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PUM

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Abstract

The invention discloses a special diluent for an alkaline phosphatase antigen-antibody. The special diluent is prepared from the following components in percentage by mass: 92 to 95 percent of phosphate buffer, 1 to 3 percent of polymer hydrophilic compound, 0.2 to 0.5 percent of sodium ethylene diamine tetracetate, 0.01 to 0.02 percent of preservative, 1 to 3 percent of modified amino acid, 0.02to 0.04 percent of an anticoagulant, 0.05 to 0.12 percent of fibroin and 2.68 to 5.72 percent of nano silver powder. Compared with the prior art, the special diluent for the alkaline phosphatase antigen-antibody, disclosed by the invention, has the advantages of simple components, good hydrophilicity and high capability of stabilizing the activity of the antigen-antibody labeling alkaline phosphatase; in addition, the required quantity is low, so that the production cost is reduced.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a special diluent for alkaline phosphatase antigen and antibody. Background technique [0002] The quality of laboratory testing is related to the definite diagnosis and condition monitoring of patients. In the field of blood infectious disease screening, the correctness of test results is also closely related to the safety of blood. Quality assessment of reagents used, indoor quality control of infectious disease testing, and participation in an appropriate external quality assessment system are a series of methods to ensure the quality of testing. However, due to the insufficient preparation process of quality control products, the stability of quality control samples used for reagent assessment, indoor quality control and external quality assessment in China is not high. The results of quality control and quality evaluation have large deviations. On the one hand, it affects the correc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/535
CPCG01N33/535
Inventor 凌云凌天阳金可心夏戊君
Owner 南京斯博伏特新材料有限公司
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