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A kind of bacillus megaterium bacterial strain zt-p and its application

A technology of Bacillus megaterium and ZT-P, which is applied in the field of agricultural microorganisms, can solve the problems of poor adaptability and insufficient activity, and achieve the effects of strong adaptability, wide application range and shortened dissolution rate.

Active Publication Date: 2020-07-28
NANJING AGRICULTURAL UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phosphorus-dissolving fungi: Aspergillus, Penicillium, but the existing phosphorus-dissolving bacteria have problems such as insufficient activity and poor adaptability

Method used

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  • A kind of bacillus megaterium bacterial strain zt-p and its application
  • A kind of bacillus megaterium bacterial strain zt-p and its application
  • A kind of bacillus megaterium bacterial strain zt-p and its application

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Experimental program
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Effect test

Embodiment 1

[0032] The screening and identification of embodiment 1 phosphate-dissolving bacteria ZT-P

[0033] Medium formula: LB Trptone 10g, Yeast extract 5g, NaCl 10g, add 15g-18g agar powder to the solid medium, distilled water to 1000mL. 121°C, 20min.

[0034] PVK medium: Glucose 10g, Ca 3 (PO 4 ) 2 5g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.2g, MgSO 4 0.1g, KCl 0.2g, yeast extract 0.5g, MnSO 4 0.002g, FeSO 4 0.002g, 0.4% bromophenol blue 6mL, distilled water to 1000mL. 121°C, 20min.

[0035] Montkina Solution Inorganic Phosphorus Medium: Glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, KCl 0.3g, NaCl 0.3g, MgSO 4 7H20 0.3g, FeS0 4 ·7H 2 O0.03g, MnS0 4 4H 2 O 0.03g, Ca 3 (PO 4 ) 2 (Inorganic phosphorus source) 10g, add 15g-18g agar powder to the solid medium with pH7.0-7.5, and distilled water to make the volume to 1000mL. 121°C, 20min. Montina solution organic phosphorus medium: Glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, KCl 0.3g, NaCl 0.3g, MgSO 4 ·7H 2 00.3g, FeSO 4 ·7H 2 O 0.0...

Embodiment 2

[0052] The germination test of the bacterial strain shaking flask supernatant of embodiment 2 phosphate-solubilizing bacteria ZT-P

[0053] Surface disinfection of plant seeds: select plump rice seeds, fully rinse with tap water, and then wash with distilled water; soak in 75% ethanol for 3 minutes, discard 75% ethanol, soak in 1.6% sodium hypochlorite for 3 minutes, and wash with sterile water for 5~ 10 times until there is no smell of sodium hypochlorite.

[0054] Inoculate the phosphate-dissolving bacteria into 100 / 250mL LB medium according to 1% inoculum amount and place it in a 37°C, 180rpm constant temperature shaker for 3D culture; centrifuge the obtained fermentation product at 10000rpm for 10min, and save the supernatant for later use . Effects of fermentation products on rice seed germination (germination rate, germination potential, root length, and bud length): 3 treatments were set up in this experiment, and each treatment was set with 3 repetitions, that is, the...

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Abstract

The invention discloses a Bacillus megaterium strain ZT-P and its application. The strain ZT-P is Bacillus megaterium. Its property is to dissolve organic phosphorus and inorganic phosphorus bacteria, and it was preserved in China on April 11, 2018. General Microbiology Center of Microbial Culture Collection Management Committee, culture preservation number CGMCC NO.15602. The bacterial strain ZT-P provided by the invention has high activity and strong adaptability, and can be widely used in the treatment of microbial fertilizers for weight loss and drug reduction in soil. The microbial fertilizer made by the strain ZT‑P was tested, and the amount of dissolved phosphorus in the shake flask reached a maximum of 156.7 μg / mL on the 5th day. Compared with before mutagenesis, the phosphorus dissolution rate of the strain increased by 20%, and the dissolution rate of organic phosphorus and inorganic phosphorus in soil was shortened. It shows that the prepared microbial organic fertilizer shortens the dissolution period and amount of organic phosphorus and inorganic phosphorus in soil.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to a Bacillus megaterium strain ZT-P with dissolved inorganic organic phosphorus and its application in soil fertilizer. Background technique [0002] Modern agricultural production has great requirements for the versatility and high efficiency of chemical fertilizers. With the continuous development of science and technology, the use of chemical fertilizers is increasing year by year, and the impact on agricultural soil is also increasing. The use of chemical fertilizers is increasing every year, followed by the utilization rate and fertilizer efficiency of chemical fertilizers are getting lower and lower, the efficiency of crop production is decreasing year by year and the use of chemical fertilizers is increasing year by year, soil fertility is also declining, and soil desertification is becoming more and more Serious; the use of pesticides is also increasing year by yea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/11
CPCC12N1/205C12R2001/11
Inventor 钟增涛曹亚君江高飞马东燕杨善忠韩福忠
Owner NANJING AGRICULTURAL UNIVERSITY
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