Application of allicin and its derivatives in the preparation of medicines for preventing and treating kidney damage caused by urinary stones and crystals

A technology of urinary calculi and allicin, applied in the field of biomedicine, achieving good application prospects and the effect of inhibiting adhesion

Active Publication Date: 2021-05-28
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, surgery is the main treatment for stones, and there are no other medicines for stone-dissolving drugs except potassium hydrogen citrate for cystine stones.

Method used

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  • Application of allicin and its derivatives in the preparation of medicines for preventing and treating kidney damage caused by urinary stones and crystals
  • Application of allicin and its derivatives in the preparation of medicines for preventing and treating kidney damage caused by urinary stones and crystals
  • Application of allicin and its derivatives in the preparation of medicines for preventing and treating kidney damage caused by urinary stones and crystals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 A certain concentration of allicin DATS and DADS has no toxic effect on renal tubular epithelial cells NRK-52E and HK-2

[0042] 1. Materials and methods

[0043] 1. Main reagents

[0044] Dulbecco's Modified Eagle's Medium dry powder (DMEM, low sugar), fetal bovine serum (FBS), trypsin (typsin), dimethyl sulfoxide (dimethyl sulfoxide, DMSO), and Tris were purchased from Sigma-Aldrich (St.Louis, MO, USA); Allicin (Diallyl disulfide, DADS) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Allicin (Diallyl trisulfide, DATS) was purchased from Guangdong Drug Control Institute. Other commonly used laboratory reagents are of domestic analytical grade.

[0045]2. Cell culture

[0046] At 37°C, 5% CO 2 And under the condition of saturated humidity, we used DMEM (low glucose) medium containing 10% fetal bovine serum to culture rat renal tubular epithelial cells NRK-52E and human renal tubular epithelial cells HK-2. The cell culture is carried out in a petri...

Embodiment 2

[0068] Example 2 Allicin and its derivatives have inhibitory effect on renal cell damage caused by sodium oxalate

[0069] 1. Experimental method

[0070] The experimental determination method of cell culture, MTT or MTS and the statistical method of other kits are the same as Example 1; 24 hours after the cells are seeded into the 96-well plate, different drug groups are set, and the different concentrations of the drug to be determined are added to the medium. Drug treatment for 24 hours. In the injury experiment caused by sodium oxalate (NaOx), allicin (DATS) was pre-added to the cells for treatment, and after 3 hours, soluble sodium oxalate of different concentrations was added to the cells (sodium oxalate combined with calcium ions in the medium Calcium oxalate crystals were formed later); 24 hours later, the corresponding MTS was added to measure the absorbance of each group, and the corresponding blank control wells were set (because calcium oxalate crystals and drugs ...

Embodiment 3

[0074] Example 3 Allicin and its derivatives have inhibitory effect on crystal adhesion after renal cell injury caused by sodium oxalate

[0075] 1. Experimental method

[0076] Materials are the same as Example 1.

[0077] Crystal adhesion experiment: First, cells were seeded into 6-well plates at a certain density (about 150,000-200,000 cells / well), and after 24 hours, the cells adhered to the wall. Change the medium and add different concentrations of allicin (DATS, DADS) and sodium oxalate (NaOx) to be measured for 24 hours. After aspirating and discarding the culture medium, add the prepared calcium oxalate suspension containing 20ug / cm2 per well, incubate at 37°C for 3 minutes, and then take out the cells. After the cells were washed 5 times with PBS, the cells were placed under a microscope to observe the cells, and the number of adhered crystals on the cell surface was counted under a field of view of 10*4. powerful.

[0078] All experiments were repeated at least ...

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Abstract

The invention discloses the application of allicin and its derivatives in the preparation of medicines for preventing and treating kidney damage caused by urinary stones and crystals. The first study of the present invention proves that allicin has the effect of inhibiting crystal-induced damage and adhesion, reducing calcium oxalate crystallization in the kidney of rat kidney stone model, and has the potential to treat urinary stones. The invention provides a new application of allicin and its derivatives, that is, its application in the prevention and treatment of kidney damage caused by urinary calculus drugs and crystals, and also provides a new option for the treatment of urinary calculus diseases, providing A diallyl sulfide and its derivatives extracted from natural garlic and other foods have been developed, which provides a research basis for garlic-based diet therapy for urinary calculi and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to the application of allicin and its derivatives in the preparation of medicines for preventing and treating renal cell damage caused by urinary stones and crystals. Background technique [0002] Urinary calculus is a common disease worldwide and the most common urological disease in my country. A prerequisite for the formation of stones in urine is the aggregation and nucleation of crystals. The study of the components of urinary calculi found that calcium oxalate is the most common chemical component of urinary calculi. The formation of calcium oxalate stones roughly includes the process of crystal nucleus formation, crystal growth, crystal aggregation, crystal adhesion and retention until stone formation. The existing hypotheses for calcium oxalate stone formation include calcium spot theory, matrix theory, supersaturation and crystallization theory , Inhib...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/255A61K31/10A61K31/095A61P13/12A61P13/04
CPCA61K31/095A61K31/10A61K31/255A61P13/04A61P13/12
Inventor 吴文起赖永长陈东钟芳灵黄健欧莉莉
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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