48 results about "Calcium Oxalate Crystals" patented technology

The invention discloses a high-yield cultivation method of interplanting of pinellia ternata in apple orchards, belonging to the technical field of interplanting cultivation. The high-yield cultivation method of interplanting of pinellia ternata in apple orchards has the following beneficial effects: apple trees are utilized to shield pinellia ternate from intense light such that pinellia ternate is protected against sprout tumble or death due to exposure under intense light, thereby decreasing production cost; gaps among apple trees are fully utilized so that land resources are saved; meanwhile, fertilizers and water resources in the process of growing pinellia ternate are utilized; without additional fertilizers and water nutrients, the nutrition demand during the growth of apple trees is met; as a result, yield of apples is increased and the incidence rate of brown spots and rotten diseases during the process of planting apples is reduced; yield of pinellia ternate is greatly improved; and the content of needle-like calcium oxalate crystal with stimulating components in pinellia ternata is also decreased, thereby ensuring medical security of pinellia ternate to the large extent.

In an embodiment of the present disclosure, there is provided a composition for dissolving calcium oxalate crystals thereby inhibiting / preventing further growth of crystals. In some embodiments, such a composition comprises at least one stereoisomer of hydroxycitrate, a derivative of the organic acid citrate. Such a method comprises administering to the subject an effective amount of the aforementioned composition. In another embodiment, the present disclosure pertains to a method of treating kidney stone disorder. Such a method comprises administering to a subject in need thereof a therapeutically effective amount of the aforementioned composition. In yet another embodiment, the present disclosure relates to a method of treating calcium oxalate stone disease. In an embodiment, the method comprises administering to a subject in need thereof a therapeutically effective amount of the aforementioned composition.

The invention discloses medicinal wine for tranquilizing mind by nourishing the heart and a preparation method thereof. The medicinal wine is prepared from the following raw materials in parts by mass: 1,000 parts of white spirit with alcohol strength of 60%, 45 to 50 parts of sealwort, 18 to 20 parts of red dates, 18 to 20 parts of spina date seed, 18 to 20 parts of fructus lycii, 18 to 20 parts of polygala tenuifolia, 18 to 20 parts of citron or 36 parts of fresh lemon, and 18 to 22 parts of momordica grosvenori. The preparation method is characterized in that polygala tenuifolia is processed by using citron or lemon to remove calcium oxalate crystal, and momordica grosvenori is used for seasoning to optimize the taste. The medicinal wine has the effect of effectively relieving insomnia, dreaminess, palpitation and other symptoms caused by anxiety and neurasthenia, has an effective rate of 100%; in addition, the medicinal wine is convenient to prepare, stable in function, safe, effective, pure in taste, and suitable for public consumers.

In an embodiment of the present disclosure, there is provided a composition for inhibiting calcium oxalate crystal growth. In an embodiment of the present disclosure, there is provided a composition for inhibiting calcium phosphate crystal growth. In some embodiments, such a composition comprises at least one citrate derivative of an organic acid and at least one pharmaceutically acceptable carrier. In another embodiment, the present disclosure relates to a method of controlling calcium oxalate crystal growth in a subject in need thereof. Such a method comprises administering to the subject an effective amount of the aforementioned composition. In another embodiment, the present disclosure pertains to a method of treating kidney stone disorder. Such a method comprises administering to a subject in need thereof a therapeutically effective amount of the aforementioned composition. In yet another embodiment, the present disclosure relates to a method of treating calcium oxalate stone disease. In an embodiment, the method comprises administering to a subject in need thereof a therapeutically effective amount of the aforementioned composition.

The invention relates to the field of pickling of foods, and particularly relates to a pickling method of pickled cowpea. The pickling method comprises steps of stir-frying, early-stage fermentation, middle-stage pickling and last-stage pickling. By adopting the raw materials and the method provided by the invention, the pickled cowpea prepared by pickling is unique in taste, short in pickling time, low in nitrate content and free of calcium oxalate crystal deposited in the urinary system so that no stone is formed to cause potential safety hazards to customers.

The invention relates to a drink composition capable of inhibiting urinary calculi formation and promoting litholysis and an application of the drink composition. The invention relates to a novel application of a garcinia cambogia extract, namely, an application of the garcinia cambogia extract in preparation of food or drugs for inhibiting urinary calculi formation and promoting litholysis. The invention also provides a drink capable of inhibiting urinary calculi formation and promoting litholysis. The drink has the advantages as follows: animal experiments find that the garcinia cambogia extract can inhibit urinary calculi formation and promote litholysis for the first time. The concentration of 24 h urinary oxalic acid and calcium ions in rats in a garcinia cambogia extract interventiongroup is significantly reduced as compared with a calculus induction group; dotted calcium oxalate crystals can be seen in kidney tubules in the garcinia cambogia extract intervention group, flaky calcium oxalate crystals are seldom seen, and the um oxalate crystal deposition and lumen expansion conditions are remarkably better than those in the calculus induction group.

The invention provides a seed dressing agent without pesticide residue and a preparation method thereof, and belongs to the field of biological products. The seed dressing agent comprises, by weight,25-30 parts of metarhizium anisopliae, 25-30 parts of beauveria bassiana, 2000-3000 parts of animal protein, 200-300 parts of brown sugar, and 15-20 parts of yeast. Animal protein and brown sugar provide nutrition for beauveria bassiana, metarhizium anisopliae and yeast in the fermentation process. Some toxins can be generated in the fermentation process of metarhizium anisopliae and has high insecticidal activity, and hyphae and spores of metarhizium anisopliae itself also have high bactericidal activity. Toxins, hydrolase, organic acids and other compounds can be generated in the fermentation process of beauveria bassiana to resist insects, and beauvericin, ovosporin and calcium oxalate crystals can also be generated to cause insect poisoning, disruption of metabolism and death. In addition, beauveria bassiana is mainly used for infecting host cells and forming specific sexual organisms after symbiosis with hosts so as to exert a pesticide effect, and beauveria bassiana can also be used for inducing cell apoptosis and the like and can be used for well preventing and treating underground insect pests.

The invention discloses a method for preparing decoction pieces of traditional Chinese medicinal material rhizoma polygonati. The method comprises the following steps: S1, preparing raw materials, namely selecting, washing, draining and slicing edible parts in raw materials; S2, pretreating, namely putting the raw materials prepared in the S1 into an acidic soaking solution, and soaking; S3, performing ultrasonic treatment, namely keeping the raw materials obtained in the S2 in the acidic soaking solution, performing ultrasonic treatment for 3-4 times; and S4, performing enzymatic honeying oncellulase, namely drying the product obtained in the S3, adding honey containing cellulase, uniformly stirring, performing enzymatic honeying for a period of time, drying, and screening chippings. According to the method, the needle-like calcium oxalate crystal in rhizoma polygonati can be degraded with a degradation rate of 87.5 percent, and the active ingredient polygahatous polysaccharides canbe well maintained with a retention rate of 99.342 percent, the taste can be improved, and the utilization rate of traditional Chinese medicinal resource rhizoma polygonati can be improved.

The invention discloses quality standard for Dieda analgesic ointment and a testing method thereof. The quality standard includes an identification item, an inspection item, a volatile oil item and a content determination item; the identification item includes microscopic identification and volatile oil identification; the microscopic identification incudes identification for crystal fiber, bast fiber, calcium oxalate crystal clusters, single cells and cell body walls; the volatile oil identification includes quantitative detection for menthol, borneol, methyl salicylate and camphor; the inspection item includes quantitative inspection for strychnine, quantitative inspection for aconitine, determination for ointment content and provisions with respect to adhesive plasters among 0122 plasters and meeting general rules of Chinese Pharmacopoeia (2015 edition); the volatile oil item includes determination for volatile oil total quantity; the content determination item includes quantitative determination for camphor by gas chromatography. The quality standard established herein and a testing method thereof are high in stability, good linear relation, high in accuracy and good in repeatability, the quality of Dieda analgesic ointment may be evaluated scientifically according to this method, and quality control for industrial production is facilitated.

The invention discloses Oxalobacter formigenes and application thereof. Oxalobacter formigenes is collected under CCTCC M2016183. The Oxalobacter formigenes can degrade oxalic acid, can decrease concentrations of oxalic acid and oxalates in human and animal blood and urea, and can effectively decrease calcium oxalate crystal; a bacterial agent prepared with Oxalobacter formigenes can be active forlong time.

The invention discloses a field processing method of fresh pinellia ternata and belongs to the technical field of processing of toxic traditional Chinese herbal medicines. The invention aims at solving the problem of toxic component residue in the pinellia ternata and aims at improving the functions of relieving cough and eliminating phlegm of the pinellia ternata. According to the field processing method of the fresh pinellia ternata, the physiological property of pinellia ternata plant cells is sufficiently used, steps such as pricking, fumigation, hypertonic solution soaking, penetrating fluid gas-tight preparation and high pressure treatment are conducted, so that the content of stimulating and toxic component calcium oxalate crystal in a prepared thin pinellia ternata sheet is lowered, the content of aspartic acid which has the functions of relieving cough and eliminating phlegm is increased, the medical value of the pinellia ternata is improved, and the medical safety of the pinellia ternata is guaranteed.

The invention relates to a plant source organic-inorganic compounding molluscicide fertilizer preparation and a preparation method and application thereof, and relates to the technical field of biological pesticides. The plant source organic-inorganic compounding molluscicide fertilizer preparation comprises a needle-like calcium oxalate crystal and an inorganic fertilizer, wherein the needle-likecalcium oxalate crystal is obtained by separating and extracting from a plant of Araceae or Amaryllidaceae. The plant source organic-inorganic compounding molluscicide fertilizer preparation also preferably includes a plant fermented organic fertilizer. A suitable amount of chemical fertilizer is added to a needle-like calcium oxalate crystal extract contained in the plant of Araceae and Amaryllidaceae, and a plant extract residue fermented product is used as an organic fertilizer carrier to prepare the plant source organic-inorganic compounding molluscicide fertilizer preparation. The plantsource organic-inorganic compounding molluscicide fertilizer preparation can not only promote rice growth, increase yield, also has the effect of killing snails and achieve the double effect of killing Oncomelania hupensis to achieve the integration of double effects of molluscicide and fertilizing. In addition, the plant source organic-inorganic compounding molluscicide fertilizer preparation issimple in preparation method, the production cost is low, the use is convenient, and the plant source organic-inorganic compounding molluscicide fertilizer preparation is conductive to large-scale application and promotion in a rice field in a schistosomiasis epidemic area.

The invention relates to the technical field of pet feed, in particular to a prescription diet for calcium oxalate calculus of dogs. The dog food mainly comprises the following raw materials in partsby weight: 70-90 parts of corn, 15-25 parts of beef, 10-15 parts of fish, 10-20 parts of duck, 5-10 parts of dried cranberries, 2-3 parts of poria cocos, 3-5 parts of semen plantaginis, 5-10 parts ofchicken oil, 5-10 parts of peanut oil, 1-2 parts of premixes and 1-5 parts of salt. The dog food provided by the invention is characterized in that the beef, the fish and the duck, which are rich in trace element, are added into the raw materials, and diuretic traditional Chinese medicinal materials such as the poria cocos and the semen plantaginis are also added; through increase of the amount ofurine of the pet dogs, the concentration of oxalic acid in the urine is reduced, and small calcium oxalate crystals are carried out of the bodies to prevent retention of the calcium oxalate calculusin the bodies.

The invention belongs to the technical field of medicines, and particularly relates to application of desmodium styracifolium extract in urinary calculus. Three positions of desmodium styracifolium are extracted, and the effect of each part on calcium oxalate crystal is studied by adopting a mouse calcium oxalate crystal model which is manufactured by injecting a glyoxylate solution into the peritoneal cavity. Results indicate that the desmodium styracifolium extract can be used for promoting the serum creatinine and urea nitrogen content of a mouse to be remarkably reduced, the calcium content of kidneys to be remarkably reduced, the calcium oxalate crystal deposition in kidney tissues and kidney pathological tissue injury to be remarkably alleviated, the levels of CAT, CuZn-SOD, GSH, osteopontin, CD44, TRPV5, Calbindin-D28 and Ki67 to be remarkably increased, and the content of MDA and TUNEL to be remarkably reduced.

The invention discloses a test method for calcium content in a tussah cocoon shell. The method comprises the steps: preparing multiple parts of EDTA or EDTA-2Na solutions by using a NaOH solution, a HCL solution, EDTA and EDTA-2Na, adding the tussah cocoon shell to react, selecting the solution with best apparent form of the tussah cocoon shell to filter so as to obtain filtrate after the reaction is finished, conducting a single-channel scanning inductively coupled plasma emission spectroscopy (ICP-AES) on the filtrate to determine the content of Ca<2+>, that is, calcium content in the tussah cocoon shell, and then calculating the calcium oxalate crystal content of the tussah cocoon shell. The design is not only high in precision and good in accuracy, but also simple in operation process and conducive to cocoon reelability of silks in the tussah cocoon.

The invention discloses a method for modifying oxalate decarboxylase by means of ethylenediaminetetraacetic dianhydride. The method comprises the steps of dissolving ethylenediaminetetraacetic dianhydride and oxalate decarboxylase in phosphate buffer, and conducting stirring reaction for 1-24 h; dialyzing the reaction product after reaction ends, and then conducting concentration to obtain ethylenediaminetetraacetic dianhydride modified oxalate decarboxylase. Chemical modification of oxalate decarboxylase is directly conducted with ethylenediaminetetraacetic dianhydride, conditions are mild, operation is easy, and cost is low; the heat stability, heat resistance, pH adaptability and trypsin tolerance of modified oxalate decarboxylase are improved; the adsorption capacity of oxalate decarboxylase on calcium oxalate crystals is improved especially; the problem that oxalate decarboxylase can be affected by gastric acid and pepsin in use to lose activity easily is solved; the practical problem that zymoprotein in enzymic preparations is diluted easily to disappear in practical use is also solved.

The invention discloses a formula for preventing and treating kidney stone by adding garcinia cambogia and roselle, the formula comprises 20-50 parts of the garcinia cambogia, 10-40 parts of the roselle, 20-50 parts of lysimachia christinae hance, 15-20 parts of plantain herb, 15-50 parts of corn stigma and 10-25 parts of lygodium japonicum, and hydroxycitrate contained in the garcinia cambogia and the roselle can not only prevent calcium oxalate crystal generation, but also can dissovle the generated stones, and the components have the effects of calming the liver, decreasing internal heat, clearing heat, diminishing inflammation, promoting the production of body fluid to quench thirst, reducing blood pressure and fat, restoring consciousness, soothing the nerves, scavenging free radicals and the like. Hibiscus syriacus acid is considered to have a certain curative effect on heart disease, hypertension, arteriosclerosis and the like, also has an anti-worm effect, can promote biliary secretion, reduce blood concentration and stimulate intestinal wall peristalsis, and has the effects of clearing heat and promoting diuresis, inducing diuresis and treating stranguria, clearing summer-heat, regulating qi and tonifying spleen, drying dampness and reducing phlegm, activating blood circulation and regulating menstruation and the like by being matched with medicinal materials such as the lysimachia christinae hance, the plantain herb, the corn stigma and the lygodium japonicum, and the kidney stones in the human body can be quickly and effectively eliminated.

The invention discloses a method for sequestering carbon dioxide, which is directly applied to the production of oxalate after trapping and absorbing carbon dioxide with an inorganic base. The oxalate is used as a detergent additive in laundry detergent. Salt and calcium ions in water form calcium oxalate crystals, thereby realizing the utilization and mineralization of carbon dioxide. The present invention absorbs carbon dioxide through carbonate and directly uses it in the production of oxalate, avoiding a large amount of energy loss in the regeneration process of the adsorbent, and the whole process can realize zero emission; the oxalate produced from carbon dioxide is used as detergent Additives, through the combination of oxalate radicals and calcium ions during the washing process to form calcium oxalate precipitation, finally realize the mineralization and storage of carbon dioxide and alleviate the greenhouse effect.

The invention discloses a preparation method of pullingqianzheng pills, and the preparation method is characterized in that 3 to 5 parts by weight of rhizoma typhonii, 3 to 5 parts by weight of stiff silkworm and 4 to 6 parts by weight of scorpio are adopted as crude drugs, and the crude drugs are sorted to remove the impurities; the scorpio is dried after being broken; the crude drugs are shattered into fine powder through an ultrafine pulverizer to be sterilized; the ultrafine powder of the crude drugs is mixed with yellow wine suspension to prepare softwood; the softwood is prepared into pills through plastic method, and then the pills are coated after being dried under the temperature of 50 to 80DEG C; and the pills are dried after being coated to obtain the pullingqianzheng pills. Due to the adoption of the preparation method, not only can biological active ingredients of the pullingqianzheng pills be maintained, but also the toxicity of the calcium oxalate crystal which is the toxic component in the rhizome typhonii can be reduced. The prepared pullingqianzheng pills hasve the advantages of high treatment effect, small dosage, small toxicity and side effect and convenience in use.

The invention discloses a formula for preventing and treating kidney stone by adding a hydroxycitric acid extract, the formula comprises 3-30 g of the hydroxycitric acid extract, 10-30 g of houttuynia cordata, 15-50 g of lophatherum gracile, 20-50 g of lalang grass rhizome and 10-30 g of pyrrosia lingua, the main effective component in the formula for preventing and treating kidney stone is hydroxycitric acid, the hydroxycitric acid can help dissolve calcium oxalate crystals, the calcium oxalate crystals is the main component of kidney stone, the hydroxycitric acid can inhibit calcium salt crystallization, and prevents kidney stone formation.

The invention relates to a traditional Chinese medicine preparation, namely, Weichangning tablets, in particular to a quality control method thereof. The quality control method comprises the following steps of: the content measurement of caulis mahoniae: each tablet contains the caulis mahoniae of not being less than 0.50mg calculated by berberine hydrochloride; identification: the Weichangning tablet is taken and arranged under a microscope for observation: epidermal tissue fragments are more, cells are square and pores are inconstant; fibers form bundles, calcium oxalate crystals are attached on the surfaces of a plurality of the fibers, and the scattered calcium oxalate cluster crystals are occasionally seen; identification of the caulis mahoniae: in the chromatogram recorded according to the content measurement item, the retention time of a main peak of a test article is consistent with the retention time of the main peak of a contrast article. The quality standards adopted by the invention can effectively supervise the production, prevent the occurrence of phenomena of wrong feed, mis-feed or more feed or less feed, being more benfiicial to the supervision of product quality, ensuring the stability of the product quality and guaranteeing the safety of drug administration of patients.

The invention discloses an intelligent identification method and system for calcium oxalate crystals based on microscopic images, and the method comprises the steps: obtaining the training data of thecalcium oxalate crystals, wherein the training data comprises various types of calcium oxalate crystals; training the training data by using a deep convolutional neural network to obtain a deep convolutional neural network model for identifying calcium oxalate crystals; performing feature matching on the microscopic image of the collected sample by using a deep convolutional neural network modelto obtain the crystal type of the calcium oxalate crystal contained in the collected sample; and generating and outputting a calcium oxalate crystal identification result of the collected sample according to the crystal type of the calcium oxalate crystal contained in the collected sample. According to the technical scheme, the problems of large error, long consumed time and low working efficiencycaused by manual identification in the prior art can be solved.

The invention discloses a test method for calcium content in a tussah cocoon shell. The method comprises the steps: preparing multiple parts of EDTA or EDTA-2Na solutions by using a NaOH solution, a HCL solution, EDTA and EDTA-2Na, adding the tussah cocoon shell to react, selecting the solution with best apparent form of the tussah cocoon shell to filter so as to obtain filtrate after the reaction is finished, conducting a single-channel scanning inductively coupled plasma emission spectroscopy (ICP-AES) on the filtrate to determine the content of Ca<2+>, that is, calcium content in the tussah cocoon shell, and then calculating the calcium oxalate crystal content of the tussah cocoon shell. The design is not only high in precision and good in accuracy, but also simple in operation process and conducive to cocoon reelability of silks in the tussah cocoon.

The invention discloses application of allicin and derivatives thereof in the preparation of a medicine for preventing and treating kidney damage caused by urinary calculi and crystals. The first study provided by the invention proves that the allicin has the effects of inhibiting crystal-induced damage and adhesion and reducing the kidney calcium oxalate crystal in a kidney stone model rat, and has the potential to treat urinary calculi. The invention provides a new usage of the allicin and the derivatives thereof, namely the application in preparation of a medicine for preventing and treating kidney damage caused by urinary calculi and crystals, also provides a new selection scheme for the treatment of urinary calculus diseases, and provides diallyl sulfide and derivatives thereof extracted from food such as natural garlic. A research basis is provided for garlic-based urinary calculus diet therapy, and the application prospect is good.

The invention relates to application of ouabain in preparation of an anti-kidney stone kidney tubule injury drug. The experiment shows that the plant-derived ouabain compound adopted can effectively improve the decline in vitality of human proximal kidney tubule epithelial cells caused by calcium oxalate monohydrate, release of lactate dehydrogenase in cells and apoptosis caused thereby. At the same time, the ouabain also can alleviate the mouse weight loss and kidney function decrease caused by glyoxylic acid, reduce the accumulation of calcium oxalate crystals in the kidney caused by glyoxylic acid, and the resulting mouse kidney tubule injury and kidney apoptosis. At present, the drug which effectively resists the kidney tubule injury caused by kidney stone is deficient, especially in the case of small molecule drugs, the ouabain has good development value and application prospects.

The invention discloses a preparation method of pullingqianzheng pills, and the preparation method is characterized in that 3 to 5 parts by weight of rhizoma typhonii, 3 to 5 parts by weight of stiff silkworm and 4 to 6 parts by weight of scorpio are adopted as crude drugs, and the crude drugs are sorted to remove the impurities; the scorpio is dried after being broken; the crude drugs are shattered into fine powder through an ultrafine pulverizer to be sterilized; the ultrafine powder of the crude drugs is mixed with yellow wine suspension to prepare softwood; the softwood is prepared into pills through plastic method, and then the pills are coated after being dried under the temperature of 50 to 80DEG C; and the pills are dried after being coated to obtain the pullingqianzheng pills. Due to the adoption of the preparation method, not only can biological active ingredients of the pullingqianzheng pills be maintained, but also the toxicity of the calcium oxalate crystal which is the toxic component in the rhizome typhonii can be reduced. The prepared pullingqianzheng pills hasve the advantages of high treatment effect, small dosage, small toxicity and side effect and convenience in use.

The invention provides a concrete protective agent, a concrete protective film and a preparation method thereof. The concrete protective agent provided by the invention comprises the following components: water, oxalic acid, defoamer and film-forming agent. When the concrete protective agent provided by the invention is used for concrete protection, the oxalic acid in the protective agent can react with the calcium ions in the concrete, and generate calcium oxalate monohydrate in situ inside and on the surface of the concrete, so as to obtain a protective film with strong bonding force with the concrete; The film-forming agent in the protective agent serves as a template to regulate the growth of calcium oxalate crystals, improving the waterproof performance and corrosion resistance of sulfate and chloride ions of the protective film. The preparation method provided by the invention is simple, practical and suitable for large-scale production.

The invention discloses a method for constructing a urinary calculus animal model by using a locust, and belongs to the field of biomedicine. The method comprises the following steps of (1) injecting4-8 [mu]L of a copper chloride solution with the concentration of 5.0-10.5 mM into the abdominal cavity of the locust for the first time, and feeding the locust with fresh wheat seedlings under the conditions that the ratio of illumination time to darkness time is 13: 11 hours, the illumination temperature is (24-28)+ / -2 DEG C, the darkness temperature is (20-26)+ / -2 DEG C and the humidity is 60-85%; (2) injecting 4-8 [mu]L of the copper chloride solution into the abdominal cavity of the locust for the second time, and feeding the locust with the fresh wheat seedlings under the conditions thatthe ratio of illumination time to darkness time is 14: 10 hours, the illumination temperature is (24-28)+ / -2 DEG C, the darkness temperature is (20-26)+ / -2 DEG C and the humidity is 60-85%; and (3) after the locust injected with the copper chloride solution is subjected to physiological metabolism for 24-48 hours, gradually generating calcium oxalate crystals in different forms in a Markov tube.The method has the advantages of low material cost, good stability, controllable process and short period.

The invention provides an identification method and system based on calcium oxalate crystal. The method comprises the following steps: firstly, acquiring a urinary sediment image; secondly, carrying out binarization segmentation on the urinary sediment image by adopting a local adaptive threshold binarization method to obtain a binary segmentation image; determining a mark image according to the binary segmentation image; determining the number of straight line segments of the edge contour of each cell region in the marked image by adopting Hough transform; and finally, identifying calcium oxalate crystals according to the number of straight line segments of the edge contour. Factors such as uneven illumination during urinary sediment image shooting are considered. Therefore, firstly, a local adaptive threshold binarization method is adopted to carry out binarization segmentation on the urinary sediment image. Then, the number of straight line segments of the edge contour of each cellregion is determined by adopting Hough transform, and finally, the calcium oxalate crystals is identified according to the number of the straight line segments of the edge contour, so that the cell regions are accurately separated, and the accuracy of identifying the calcium oxalate crystals is further improved.