Method for constructing urinary calculus animal model by using locust

A technology of urinary stones and animal models, applied in the field of biomedical research, can solve problems such as obstacles and restrictions on effective medical treatment, and achieve high efficiency, economic convenience, and good test repeatability

Active Publication Date: 2020-08-14
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The limitations of these models have hindered and limited effective medical treatment, preventive measures, and medical translation applications to a certain extent.

Method used

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  • Method for constructing urinary calculus animal model by using locust

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Catch O. sinensis adults from the paddy field, put them into a ventilated sarong and then place them in a climate incubator and feed them adaptively with fresh wheat seedlings for 2-3 days. The optimal feeding environment conditions are: the light and dark time is 13:11 hours , the temperature in light is 25±2℃, the temperature in darkness is 21±1℃, and the humidity is 60-65%;

[0024] 2. Precisely configure the formula of standard insect saline: NaCl: 0.138 M, KCl: 0.0027 M, NaCl: 0.0027 M, NaCl 2 HPO 4 : 0.0073M, KH 2 PO 4 : 0.00147 M, pH=7.4; the prepared insect saline was placed in a Gaoyan sterilizer and sterilized by steam at 120°C for 20 minutes;

[0025] 3. Prepare a 10.5 mM copper chloride solution with the above-mentioned sterilized insect saline;

[0026] 4. Randomly select the rice locusts that have been adaptively reared, spray 75% alcohol on their body surface for disinfection, and use a micro-syringe to draw 75% alcohol to rinse the inner tube of t...

Embodiment 2

[0034] Example 2: In this example, the concentration of the copper chloride solution injected into the abdominal cavity of Oryza chinensis is 7.0 mM, and the injection volume is 5 μL. 2°C, the temperature in the dark is 20±2°C, the humidity is 70-75%, and other operations are the same as in Example 1.

Embodiment 3

[0035] Example 3: In this example, the concentration of the copper chloride solution injected into the abdominal cavity of Oryza chinensis was 8.0 mM, and the injection volume was 6 μL. The feeding conditions and other operations of Oryza chinensis were the same as in Example 1.

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Abstract

The invention discloses a method for constructing a urinary calculus animal model by using a locust, and belongs to the field of biomedicine. The method comprises the following steps of (1) injecting4-8 [mu]L of a copper chloride solution with the concentration of 5.0-10.5 mM into the abdominal cavity of the locust for the first time, and feeding the locust with fresh wheat seedlings under the conditions that the ratio of illumination time to darkness time is 13: 11 hours, the illumination temperature is (24-28)+/-2 DEG C, the darkness temperature is (20-26)+/-2 DEG C and the humidity is 60-85%; (2) injecting 4-8 [mu]L of the copper chloride solution into the abdominal cavity of the locust for the second time, and feeding the locust with the fresh wheat seedlings under the conditions thatthe ratio of illumination time to darkness time is 14: 10 hours, the illumination temperature is (24-28)+/-2 DEG C, the darkness temperature is (20-26)+/-2 DEG C and the humidity is 60-85%; and (3) after the locust injected with the copper chloride solution is subjected to physiological metabolism for 24-48 hours, gradually generating calcium oxalate crystals in different forms in a Markov tube.The method has the advantages of low material cost, good stability, controllable process and short period.

Description

technical field [0001] The invention belongs to the field of biomedical research, and in particular relates to a method for constructing an animal metabolic urinary calculus model by using Oryza chinensis. Background technique [0002] Metabolic urolithiasis (kidney stones or urolithiasis) is a common disease in the urinary system of animals including humans. Surveys show that the incidence of stones in different regions is between 3% and 12%. After long-term research by medical workers The main component of metabolic urolithiasis was determined to be calcium oxalate, which alone or together with other calcium salts constitutes urolithiasis in animals. Due to the particularity of human beings as medical objects and scientific research materials, the research on related urinary calculi needs to rely on other animals as substitutes and experimental models, select appropriate animals and model them through standardized methods and procedures, and induce or coerce animals to pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A61D7/00
CPCA01K67/033A61D7/00
Inventor 刘耀明
Owner SHANXI UNIV
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