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Oxalobacter formigenes and application thereof

A technology for oxalic acid bacteria and formic acid production, applied in the field of microorganisms, can solve the problem of low ability to degrade oxalic acid, and achieve the effects of reducing calcium oxalate crystallization and increasing biomass

Inactive Publication Date: 2019-03-01
SHANGHAI SINE PHARMA LAB +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a kind of oxalobacillus formate and its application aiming at the low ability of oxalic acid-degrading oxalic acid in the prior art.

Method used

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  • Oxalobacter formigenes and application thereof
  • Oxalobacter formigenes and application thereof
  • Oxalobacter formigenes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The screening of embodiment 1 producing formic acid oxalobacillus

[0031] 1. Material preparation

[0032] (1) Bacterial source

[0033] Bacterial source: Feces of healthy Chinese children (without infection history for three months).

[0034] (2) culture medium

[0035] Medium 1: potassium dihydrogen phosphate 0.25g / L, dipotassium hydrogen phosphate 0.25g / L, sodium acetate 0.9g / L, ammonium sulfate 0.8g / L, sodium oxalate 1g / L, yeast powder 5g / L and chloride Calcium 1g / L.

[0036] Medium 2: potassium dihydrogen phosphate 0.25g / L, dipotassium hydrogen phosphate 0.25g / L, sodium acetate 0.9g / L, ammonium sulfate 0.8g / L, sodium oxalate 1g / L and yeast powder 5g / L.

[0037] Medium 1 and 2 were both sterilized at 115° C. for 20 minutes; 2% (mass ratio) agar was added when using the above medium to prepare a solid plate (medium 1).

[0038] (3) Experimental equipment

[0039] Table 1 Summary of Experimental Instruments

[0040]

[0041]

[0042] 2. Isolation and scree...

Embodiment 2

[0099] Example 2 Fermentative preparation of oxalobacterium formigenes (Oxalobacter formigenes) SSYG-15

[0100] Prepare oxalobacter formigenes (Oxalobacter formigenes) SSYG-15 according to the following steps:

[0101] (1) Prepare 250L of fermentation medium in a 500L fermenter, and steam sterilize at 115°C for 20min;

[0102] (2) During the sterilization and cooling process, feed protective gas (carbon dioxide, nitrogen, hydrogen, argon or helium) at a flow rate of 25 L / h until the fermentation broth is cooled to room temperature, and adjust the flow rate to 10 L / h;

[0103] (3) inoculate the seed liquid in the fermenter by 1%, set the tank temperature to be 37°C, and stir at a speed of 100rpm;

[0104] (4) sampling and measuring the thalline content in its fermented liquid every 12h;

[0105] (5) After 72 hours of fermentation, the cell concentration reached 2.1×10 8 CFU / mL;

[0106] (6) Take the above fermented liquid and carry out centrifugation at 4°C to obtain the b...

Embodiment 4

[0114] Example 4 Use of Oxalobacter formigenes SSYG-15 to reduce urinary oxalate excretion and kidney calcium oxalate crystal formation in rats with calcium oxalate stones

[0115] (1) Animals: male Sprague Dawley rats weighing 180-200g;

[0116] (2) Food: conventional feed;

[0117] (3) Administration method: gavage;

[0118] (4) Test plan:

[0119] Blank control group (n=7): fed with sterile pure water and free to drink.

[0120] Calcium oxalate stone modeling group (n=7): feeding medium and sterile pure water + 0.8% ethylene glycol, free drinking water, rats were given PBS as a placebo at 5 pm every day, and free drinking water.

[0121] SSYG-15 10 8 cfu treatment group (n=7): feeding SSYG-15 fermentation liquid (10 7 cfu / ml) 1ml and sterile pure water + 0.8% ethylene glycol, the rats were gavaged at 5:00 p.m. every day and had free access to water.

[0122] DSM 4420 10 8 cfu treatment group (n=7): feeding DSM 4420 fermented liquid (10 8 cfu / ml) 1ml and sterile pure...

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Abstract

The invention discloses Oxalobacter formigenes and application thereof. Oxalobacter formigenes is collected under CCTCC M2016183. The Oxalobacter formigenes can degrade oxalic acid, can decrease concentrations of oxalic acid and oxalates in human and animal blood and urea, and can effectively decrease calcium oxalate crystal; a bacterial agent prepared with Oxalobacter formigenes can be active forlong time.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to an Oxalobacter formigenes and an application thereof. Background technique [0002] Calcium oxalate stones are the most common type of kidney stones, accounting for 70-80% of all stone types, and they tend to recur after treatment. Excessive excretion of oxalate in urine is an important pathological factor for the formation of calcium oxalate stones. Both endogenous metabolites and exogenous oxalic acid intake can cause hyperoxaluria. It has been confirmed that 46% of patients with idiopathic calcium oxalate stones have increased intestinal oxalate absorption. However, patients with hereditary hyperoxaluria still lack effective treatment drugs, and develop end-stage renal failure at an average age of 37. Therefore, increasing the degradation and excretion of oxalate in the intestine may be one of the effective methods to prevent and treat calcium oxalate stones. [0003] Oxalobac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/741A61P13/04A61P13/00A61P13/12C12R1/01
CPCA61P13/00A61P13/04A61P13/12C12N1/20A61K2035/115C12R2001/01C12N1/205
Inventor 文彬梅长林孙宁云汤晓静高远于鸿晶
Owner SHANGHAI SINE PHARMA LAB
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