Application of omeprazole in preparation of liver cancer stem cell inhibitor

A technology of liver cancer stem cells and omeprazole, which is applied in the direction of medical preparations containing active ingredients, organic active ingredients, drug combinations, etc., can solve the problem of lack of liver cancer stem cell inhibitory drugs

Inactive Publication Date: 2018-10-23
CAMOD BIOLOGICAL TECH TIANJIN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides the application of omeprazole for the preparation of liver cancer stem cell inhibitors, so as to solve the technical problem of lacking a liver cancer stem cell inhibitory drug in the prior art

Method used

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  • Application of omeprazole in preparation of liver cancer stem cell inhibitor
  • Application of omeprazole in preparation of liver cancer stem cell inhibitor
  • Application of omeprazole in preparation of liver cancer stem cell inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A liver cancer stem cell inhibitor, which contains omeprazole; the effective concentration of the liver cancer stem cell inhibitor is evaluated by the concentration of omeprazole, and the effective concentration of omeprazole is 0.2mg / mL. The preparation method of the liver cancer stem cell inhibitor is as follows: take 1100 g of starch and 400 g of water to make starch slurry, add 230 g of omeprazole to the starch slurry and mix evenly, then granulate, sieve, dry, and pack into capsules.

Embodiment 2

[0028] A liver cancer stem cell inhibitor, which contains omeprazole, catechin, paromomycin sulfate, ribavirin, said omeprazole, catechin, paromomycin sulfate, ribavirin The mass ratio of the four is 23:1:3:8. The effective concentration of the liver cancer stem cell inhibitor is evaluated by the concentration of omeprazole, and the effective concentration of omeprazole is 0.2 mg / mL. The preparation method of this liver cancer stem cell inhibitor is as follows: take starch 1100g and 400g water to make starch slurry, add omeprazole 230g, catechin 10g, paromomycin sulfate 30g, ribavirin 80g to this starch slurry Mix well, then granulate, sieve, dry and pack into capsules.

Embodiment 3

[0030] This example is used to investigate the inhibitory effect of the liver cancer stem cell inhibitors provided in the above examples on the growth of liver cancer stem cells and liver solid tumors.

[0031] 1. Materials and reagents

[0032] Cell culture medium (DMEM) and fetal bovine serum were purchased from Invitrogen; Taq enzyme (Hot Start Version) and dNTPs were purchased from TaKaRa; lipoprotein-depleted serum (LPDS) was prepared from fetal bovine serum by ultracentrifugation.

[0033] Human hepatoblastoma cell line HepG2 and human liver cancer cell line Huh7 were purchased from Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, and cultured in Dulbecco's modified Eagle's medium (DMEM) medium containing 10% fetal bovine serum. Add 100 U / ml ampicillin and 100 μg / ml streptomycin to the culture medium, and keep the cells at 37°C and 5% CO 2 cultivated under conditions. The experimental medium was DMEM medium containing 5% LPDS.

[0034] Liver ca...

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Abstract

The invention provides application of omeprazole in preparation of a liver cancer stem cell inhibitor. According to the technical scheme, CD133 is used as a molecular marker to sort so as to obtain LCSC cells at first, an animal transplantation tumor model is constructed, and then the inhibition effect of the omeprazole to liver cancer stem cells is inspected. Results show that by application of the omeprazole, proliferation of the LCSC cells can be inhibited directly, and the proliferation rate of solid tumor is reduced; moreover, the liver cancer stem cell inhibitor which contains the omeprazole has certain targeting on LCSC cell inhibition effect, and effect of inhibiting common liver cancer cells is relatively weak; and meanwhile, it further discovers that a certain synergism can be achieved for the inhibitor under the conditions that ingredients such as catechin and ribavirin are added. On the basis of the beneficial discovery, application of the omeprazole in preparation of the liver cancer stem cell inhibitor is determined, therefore, a new way is provided for treatment of tumors of livers, and meanwhile, the medical application range of the omeprazole is expanded.

Description

technical field [0001] The invention relates to the technical field of chemical medicines, and further relates to the new medical application of substances, in particular to the application of omeprazole in the preparation of liver cancer stem cell inhibitors. Background technique [0002] Stem cells are a kind of pluripotent cells with self-replication ability, which can differentiate into various functional cells under certain conditions. Stem cells have high hopes in cell replacement and tissue regeneration therapy due to their proliferation and differentiation characteristics. They can not only be used for the treatment of diseases caused by definite cell type lesions such as neurodegeneration and diabetes, but also can be used in tissue engineering and organ regeneration. Reengineering technology is used in tissue repair and organ transplantation, as well as new drug development, gene therapy, immune regulation therapy and other fields. Cancer stem cells, also known as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4439A61K31/353A61K31/7036A61K31/7056A61P35/00
CPCA61K31/353A61K31/4439A61K31/7036A61K31/7056A61P35/00A61K2300/00
Inventor 邢继亮江志华
Owner CAMOD BIOLOGICAL TECH TIANJIN CO LTD
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