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Ascorbic acid electrochemiluminescence determination method

A technology of electrochemistry and ascorbic acid, applied in analytical chemistry and nanometer fields, can solve the problems of affecting the measurement results, unfavorable rapid analysis, complicated steps, etc., and achieve the effects of high accuracy, low cost, and simple production.

Inactive Publication Date: 2018-10-23
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most require high experimental conditions and operating techniques, and some methods are cumbersome and not conducive to rapid analysis
In addition, some methods of analysis by means of color will also affect the results of the determination due to the color of the sample to be tested

Method used

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  • Ascorbic acid electrochemiluminescence determination method
  • Ascorbic acid electrochemiluminescence determination method
  • Ascorbic acid electrochemiluminescence determination method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Add 0.6 mL of 0.5 mol / L sodium hydroxide and 0.4 mL of 20 mg / mL chloroauric acid solution to 4 mL of 0.08 mol / L N-acetyl-L-cysteine ​​solution, mix After uniformity, they were incubated in a constant temperature water tank at 37 °C for 3 h. After the reaction, the reaction solution was purified by dialysis with a dialysis bag with a molecular cut-off of 3500 to obtain an aqueous solution of N-acetyl-L-cysteine-gold nanoclusters, which was freeze-dried to obtain N-acetyl-L-cysteine Acid-gold nanocluster powder.

Embodiment 2

[0051] Glassy carbon electrodes with a diameter of 3 mm were coated with 1.0 μm, 0.3 μm and 0.05 μm Al 2 o 3 The powder is polished and polished in turn until it reaches a smooth mirror surface, and then put in HNO in turn 3 solution, absolute ethanol, ultrasonic cleaning in deionized water for 3 minutes, N 2 blow dry. Take 5 μL of the N-acetyl-L-cysteine-gold nanocluster solution prepared in Example 1 and drop it on the surface of the treated glassy carbon electrode, and dry it at room temperature to obtain N-acetyl-L-cysteine-gold nanocluster solution Gold nanoclusters modified glassy carbon electrodes. The N-acetyl-L-cysteine-gold nanocluster modified glassy carbon electrode was soaked in 0.1 mol / L sodium borohydride solution for 5 min to obtain the gold nanocluster probe modified electrode. The above-mentioned gold nanocluster probe modified electrode was used as the working electrode, the platinum wire was used as the counter electrode, and the Ag / AgCl electrode was u...

Embodiment 3

[0053] Glassy carbon electrodes with a diameter of 3 mm were coated with 1.0 μm, 0.3 μm, 0.05 μm Al 2 o 3 The powder is polished and polished in turn until it reaches a smooth mirror surface, and then put in HNO in turn 3 solution, absolute ethanol, ultrasonic cleaning in deionized water for 3 minutes, N 2 blow dry. Take 5 μL of the N-acetyl-L-cysteine-protected gold nanocluster solution prepared in Example 1 and drop it on the surface of the treated glassy carbon electrode, dry it at room temperature, and further soak the electrode in 0.1 mol / L react in sodium borohydride solution for 5 minutes to obtain a gold nanocluster probe modified glassy carbon electrode. A three-electrode system was adopted, with gold nanocluster probe-modified glassy carbon electrode as the working electrode, platinum wire electrode as the counter electrode, and Ag / AgCl as the reference electrode. 4 -H 2 SO 4 (1:1 V / V) solution, manganese dioxide was electrodeposited, the potential was -0.2 V, ...

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Abstract

The invention discloses an ascorbic acid electrochemiluminescence determination method. According to the ascorbic acid electrochemiluminescence determination method of a gold nano-cluster probe, the high-quantum-yield gold nano-cluster electrochemiluminescence probe is prepared by a reduction method, a manganese dioxide nano-material serves as an electrochemiluminescence quenching agent, electrochemiluminescence signals are restored by oxidation-reduction reaction of ascorbic acid and manganese dioxide, and the content of the ascorbic acid is measured. Within the concentration range of 1.0*10<-9>-1.0*10<-2>mol / L, the logarithm value of the concentration of the ascorbic acid and the changing value of relative electrochemiluminescence strength have a linear relation, and detection limit is 2.56*10<-10>mol / L. Moreover, the ascorbic acid electrochemiluminescence determination method is simple to operate, high in sensitivity and good in reproducibility and selectivity, and accordingly has agood application prospect.

Description

technical field [0001] The invention relates to an ascorbic acid electrochemiluminescence assay method for a gold nano cluster probe, which belongs to the field of analytical chemistry and nanotechnology. Background technique [0002] Ascorbic acid (ascorbic acid), also known as vitamin C, widely exists in food and plant tissues, is an important antioxidant, and is also one of the important vitamins for maintaining normal physiological functions of the body. Ascorbic acid is widely involved in a series of metabolism and redox reactions in the body, and helps to synthesize intercellular substances such as collagen and mucopolysaccharides. It is also indispensable in the synthesis of adrenal cortex hormones and redox reactions in the body. The lack of human ascorbic acid can lead to scurvy, so sensitive and selective detection of ascorbic acid is of great significance in the fields of medicine and food. [0003] Commonly used methods for the determination of ascorbic acid inc...

Claims

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Application Information

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IPC IPC(8): G01N21/76
CPCG01N21/76
Inventor 彭花萍黄种南查代君邓豪华陈伟
Owner FUJIAN MEDICAL UNIV
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