Composition for replacing radix bupleuri to prepare glioma treatment medicine and determination method of composition

A technology for glioma and therapeutic drugs, which is used in the field of preparation of glioma therapeutic drugs instead of Bupleuri Coniferae and its determination, and can solve the problems of difficult quality, complex components, and unclear active ingredients, etc. question

Inactive Publication Date: 2018-11-02
杨真慧
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Problems solved by technology

[0003] Using roots as medicine has the disadvantages of complex ingredients, unclear medicinal ingredients, and difficult quality control

Method used

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  • Composition for replacing radix bupleuri to prepare glioma treatment medicine and determination method of composition
  • Composition for replacing radix bupleuri to prepare glioma treatment medicine and determination method of composition
  • Composition for replacing radix bupleuri to prepare glioma treatment medicine and determination method of composition

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Embodiment Construction

[0046] The substantive content of the present invention will be described in detail below in conjunction with the accompanying drawings and embodiments, but the protection scope of the present invention is not limited thereto.

[0047] 1. Experimental materials

[0048] Bupleurum chinensis was collected from Hailaer, Inner Mongolia, dried at 55°C overnight after crushing, and stored in a dry place for future use.

[0049] Agilent 1100 liquid chromatograph; Sartorius BS210S electronic balance, Beijing Sartorius Instrument System Co., Ltd.; Sartorius CP225D electronic balance, Beijing Sartorius Instrument System Co., Ltd.; acetonitrile (chromatographically pure) and methanol (chromatographically pure) were purchased from TEDIA; the water is Wahaha purified water.

[0050] RNAiso plus reagent, SYBRGreen PCRMaster Mix and PrimeScript RT kit were purchased from TaKaRa.

[0051] 2. Experimental method

[0052] 1. Determination of saikosaponin F, saikosaponin G, saikosaponin I, sa...

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Abstract

The invention discloses composition for replacing radix bupleuri to prepare a glioma treatment medicine and a determination method of the composition. The composition consisting of saikosaponins F, G,C, A and D can basically equivalently replace the inhibition effect of the radix bupleuri to expression of an mTOR gene and has the same intensity with the radix bupleuri. The composition can equivalently replace the radix bupleuri to prepare an mTOR gene expression inhibitor. The skilled in the art know that inhibition of the expression of the mTOR gene can effectively inhibit proliferation of glioma cells (miRNA-99b negatively regulates the invasion ability of mTOR in inhibiting glioma cells, China Pharmacology General, volume 34, number 4, in 4, 2018), so that the composition can be used for equivalently replace the radix bupleuri to prepare the glioma treatment medicine.

Description

technical field [0001] The invention relates to a composition used to replace Bupleuri Coniferae to prepare tumor therapeutic drugs and an assay method thereof. Background technique [0002] Bupleurum bicaule Helm is the dried root of Bupleurum bicaule Helm in the genus Bupleurum bicaule Helm of the family Bupleurum. Saponins are the main active ingredients of Bupleurum genus medicinal plants. Modern pharmacological studies have shown that saikosaponins have anti-inflammatory, analgesic and sedative, liver-protecting, anti-depressant, and anti-cancer effects. [0003] Using roots as medicine has the disadvantages of complex components, unclear efficacy components, and difficult quality control. If a composition that can replace Bupleuri Coniferae as medicine can be found, a chemical blend can be used instead of Bupleurum Coniferae as medicine, with clear ingredients and controllable quality. [0004] Bupleurum chinensis mainly contains saikosaponin F, saikosaponin G, saiko...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61K31/704A61P35/00G01N30/02
CPCA61K31/7048A61K31/704A61P35/00G01N30/02A61K2300/00
Inventor 杨真慧
Owner 杨真慧
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