A method of reprogramming fibroblasts into sertoli cells in vitro and its application

A technology of fibroblasts and fibroblasts, applied in the field of cell culture, can solve the problem of unrealistic large-scale acquisition of Sertoli cells
CN108795874BActive Publication Date: 2022-04-26TSINGHUA UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TSINGHUA UNIV
Publication Date
2022-04-26

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Abstract

The invention discloses a method for reprogramming fibroblasts into Sertoli cells in vitro and its application. The method comprises the following steps: introducing into the fibroblasts a fluorescent protein reporter system specifically expressed in Sertoli cells; then introducing substances that improve the expression level and / or activity of the NR5A1 protein; adding G418, culturing for 3-7 days; discarding the liquid phase , add the medium for culturing fibroblasts, and continue culturing for 3‑7d; separate Sertoli cells from the culture system. Experiments have proved that the cells prepared by the above method have completely obtained the characteristics of Sertoli cells, such as attracting endothelial cells, forming lipid droplets, interacting with germ cells, inhibiting the growth of lymphocytes and interleukin production, and immune immunity. . The invention has important application value in the fields of infertility treatment, allogeneic transplantation, cell therapy and the like.
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Description

technical field

[0001] The invention belongs to the field of cell culture, and in particular relates to a method for reprogramming fibroblasts into Sertoli cells in vitro and an application thereof. Background technique

[0002] According to the World Health Organization, infertility afflicts about 15% of couples in the world. Among them, male factors account for 40%-60% of the proportion, which is specifically reflected in the number or quality of sperm cells. Human pluripotent stem cells have the potential to differentiate into various cell types in the human body. In vitro differentiation of stem cells into functional sperm cells provides a feasible approach for the treatment of infertility.

[0003] Sertoli cells are a kind of testicular somatic cells, which exist together with germ cells in the seminiferous tubules and play a pivotal role in the triggering and regulation of spermatogenesis. For the construction of sperm in vitro differentiation system, Sertoli cells ...

Claims

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