The invention discloses a traditional Chinese medicine composition used for treating immune infertility, and a preparation method and application thereof. According to the composition, with Mongolian milkvetch root, largehead atractylodes rhizome, Asiatic cornelian cherry fruit, and medlar, spleen invigorating, kidney nourishing, immunity enhancing, and abnormal immune suppression effects are provided; with salvia miltiorrhiza, blue turmeric rhizome, red peony root, peony tree root bark, pangolin, cowherb seed, and white peony root, blood circulation is activated, stasis is removed, excessive antigens can be removed, immune complex generation can be prevented, and blood circulation is improved; with paniculate swallowwort, ramie root, divaricate saposhnikovia, Mongolian milkvetch root, and black cicada slough, antibody generation is inhibited, and good desensitization effect is provided; and with glabrous greenbrier rhizome, coix seed, licorice root, and giant knotweed rhizome, heat clearing, detoxifying, dampness removing, and humoral immune inhibition effects are provided. As results of clinical experiments, the traditional Chinese medicine composition provided by the invention has good immune infertility treatment effect, and is safe and reliable. The composition can be widely applied in treatments of immune infertility.