Microbial bacterial manure with ascophyllum nodosum as matrix

A technology of microbial fertilizer and microbial bacteria, applied in the field of microbial fertilizer, can solve the problems of no optimal effect and optimal bacterial agent, etc., achieve good field practical effect, increase yield, and improve fertilizer utilization rate

Inactive Publication Date: 2018-11-23
安徽金敦福农业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the shortcoming that Ascophyllum nodosum can not exert the maximum effect when used alone, adding microbial inoculum can improve the fertilizer utilization rate of Ascophyllum nodosum residue, but there has not been a unified optimal effect and selection of the best inoculum. The present invention mainly solves the problem of The coordination relationship between microbial agents and Ascophyllum nodosum, the optimal microbial fertilizer for Ascophyllum nodosum

Method used

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  • Microbial bacterial manure with ascophyllum nodosum as matrix
  • Microbial bacterial manure with ascophyllum nodosum as matrix
  • Microbial bacterial manure with ascophyllum nodosum as matrix

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Embodiment

[0029] (1) The microbial bacteria are Bacillus subtilis and Pseudomonas preserved on a glycerol slant, the ratio is 1:1; the microbial bacteria are cultured on the basal medium at 32°C and shaken at 180r / min for 18h, The bacterial liquid is obtained, and then the bacterial liquid is inoculated in the sterilized seed culture liquid according to the inoculum amount of 5% by volume, and shaken at 100r-150r / min for 16-20h under the condition of 30°C to obtain the seed liquid.

[0030] The basal medium is obtained by mixing 4g of beef extract, 10g of peptone, 5g of NaCl, 15g of agar and 1000ml of water, heating to 90°C and stirring for 30min until the nutrients are dissolved, adjusting the pH to 7.5, and cooling at room temperature.

[0031] The seed culture solution is obtained by mixing 45 g of glucose, 30 g of soybean powder, 5 g of yeast powder, 4.0 g of CaCO and 1000 ml of distilled water, and sterilizing at 121° C. for 20 minutes.

[0032] (2) At 30°C, put the seed solution i...

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Abstract

The invention discloses microbial bacterial manure with ascophyllum nodosum as a matrix. The microbial biological manure is prepared by the steps: activating and culturing microbial flora to obtain aseed solution, performing amplification culture on the seed solution to obtain a microbial bacterial agent, then mixing and feeding raw materials into a stirring tank according to a weight ratio of 30% to 70% of ascophyllum nodosum dreg, 1% to 20% of soya bean cake and 1% to 5% of microbial bacterial agent, keeping a water content as 40 to 60%, bagging after the materials are evenly stirred, sealing an opening, obtaining finished product stacking, continuing fermentation and naturally degrading for 7 to 15d to obtain the microbial bacterial manure. The microbial bacterial manure not only can improve soil and improve soil breathability and agglomeration capacity, but also can stimulate plant to generate growth regulating matter (polyamine) and formation of antibiotic (plant antitoxin) afterbeing absorbed by plants.

Description

technical field [0001] The invention relates to the technical field of green biological fertilizers, in particular to a microbial fertilizer based on Ascophyllum nodosum. Background technique [0002] Ascophyllum nodosum belongs to the Phaeophyta and is mainly grown on the coast near the Arctic Ocean. Seaweed fertilizer is made of large fast-growing brown algae that grows in the ocean as raw material. Through chemical, physical or biological methods, the active ingredients in seaweed are extracted to make fertilizer and given to plants as nutrients. Seaweed fertilizer contains various macroelements, trace elements, seaweed polysaccharides, highly unsaturated fatty acids and various natural plant growth regulators, such as auxins, gibberellins, cytokinins, and polyphenolic compounds. and antibiotics etc. These substances have high biological activity, can stimulate the production of non-specific active factors in plants and regulate the balance of endogenous hormones, can p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F17/00C05G3/00C05G3/04C12N1/20C12R1/125C12R1/38C05G3/80
CPCC05F5/002C05F17/00C05G3/00C05G3/80C12N1/20C05F11/00C05F11/08Y02W30/40
Inventor 戴乐天邓大鹏蔡永欢
Owner 安徽金敦福农业科技有限公司
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