Microbial bacterial manure with ascophyllum nodosum as matrix
A technology of microbial fertilizer and microbial bacteria, applied in the field of microbial fertilizer, can solve the problems of no optimal effect and optimal bacterial agent, etc., achieve good field practical effect, increase yield, and improve fertilizer utilization rate
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[0029] (1) The microbial bacteria are Bacillus subtilis and Pseudomonas preserved on a glycerol slant, the ratio is 1:1; the microbial bacteria are cultured on the basal medium at 32°C and shaken at 180r / min for 18h, The bacterial liquid is obtained, and then the bacterial liquid is inoculated in the sterilized seed culture liquid according to the inoculum amount of 5% by volume, and shaken at 100r-150r / min for 16-20h under the condition of 30°C to obtain the seed liquid.
[0030] The basal medium is obtained by mixing 4g of beef extract, 10g of peptone, 5g of NaCl, 15g of agar and 1000ml of water, heating to 90°C and stirring for 30min until the nutrients are dissolved, adjusting the pH to 7.5, and cooling at room temperature.
[0031] The seed culture solution is obtained by mixing 45 g of glucose, 30 g of soybean powder, 5 g of yeast powder, 4.0 g of CaCO and 1000 ml of distilled water, and sterilizing at 121° C. for 20 minutes.
[0032] (2) At 30°C, put the seed solution i...
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