Jujube flower extract and antibacterial application thereof

A technology of extract and jujube flower, which is applied in the field of jujube flower extract and its preparation, can solve the problem of no research reports on active chemical components, and achieve good antibacterial activity

Inactive Publication Date: 2018-11-30
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although there are many studies on the jujube part of the fruit of the jujube tree, the research on the jujube flower of the jujub...

Method used

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  • Jujube flower extract and antibacterial application thereof
  • Jujube flower extract and antibacterial application thereof
  • Jujube flower extract and antibacterial application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] After crushing 5kg jujube flowers, add 15kg of ethanol water solution with a volume concentration of 90% for cold soaking, each cold soaking time is 48h, and cold soaking is 2 times in total; the cold soaking liquid is concentrated under reduced pressure at 35°C to obtain an extract About 760g; the extract was dispersed and extracted with ethyl acetate and water, and the ethyl acetate phase was concentrated under reduced pressure at 35°C to obtain 200g of jujube flower extract.

[0016] 200 g of jujube flower extract was mixed with 300 g of silica gel and loaded into a column for silica gel column chromatography with a column volume of 1.5 L. Use chloroform / methanol as the eluent for gradient elution, the elution gradients are 100:1, 60:1, 30:1, 5:1, 1:1, 0:1, and two column volumes are collected for each gradient Each effluent of gained is analyzed and detected by silica gel thin-layer plate, and the combined chloroform / methanol volume ratio is the eluent of 30:1 and 3...

Embodiment 2

[0020] The antibacterial activity of the jujube flower extract of embodiment 1

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PUM

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Abstract

The invention discloses jujube flower extract and an antibacterial application thereof. The extract is obtained in the following steps: crushing the jujube flowers, soaking the crushed jujube flowersfor twice by using an ethanol aqueous solution with the volume concentration of 90 percent according to a material-liquid ratio of 1:2 to 1:4, wherein the soaking time for each time is 24 to 72 hours;decompressing concentrating the soaking solution at the temperature of 30 to 50 DEG C, and obtaining extract; and extracting the extract by using ethyl acetate and water in a dispersion manner, and decompressing concentrating an ethyl acetate phase at 25 to 40 DEG C, and obtaining the jujube flower extract. By virtue of the activity screening of the jujube flower extract as well as a monomer compound for the seven selected bacteria (grampositive staphylococcus aureus ATCC 43300, staphylococcus aureus ATCC 33591, staphylococcus aureus ATCC 29213, staphylococcus aureus ATCC 25923, enterococcusfaecalis ATCC 51299, enterococcus faecium ATCC 35667 and escherichia coli ATCC 25922), the jujube flower extract has good antibacterial activity, and can be used as an antibacterial agent.

Description

technical field [0001] The invention relates to a chemical active ingredient extracted from plant flowers and its preparation and use, in particular to a jujube flower extract and its preparation and use. Background technique [0002] Jujube is a plant of the genus Jujube in the family Rhamnaceae. It is native to China and has a planting history of more than 4,000 years in my country. It is a traditional dominant tree species in our country. Jujube plants are found in tropical, subtropical and temperate regions. There are about 170 species in the world, and there are about 14 species in my country. At present, my country's jujube cultivation area is as high as 1.5 million hectares, with an annual output of 3 million tons, accounting for more than 99% of the world's total jujube output. Jujube trees occupy a very important position in my country because of their early fruit and high yield, rich nutrition, drought resistance, barren tolerance, remarkable ecological and econom...

Claims

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Application Information

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IPC IPC(8): C07J9/00C07J63/00C07J17/00C07H1/08C07H17/07C07D311/30C07D311/40C07C51/42C07C59/64A01P1/00A61P31/04
CPCA61P31/04C07C51/42C07C59/64C07D311/30C07D311/40C07H1/08C07H17/07C07J9/00C07J17/005C07J63/008Y02A50/30
Inventor 刘庆超郭甜甜白乃生刘浪浪李国林刘甜韩稳荣卢英辉周鑫白吉祥
Owner NORTHWEST UNIV(CN)
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