Jujube flower extract and antibacterial application thereof
A technology of extract and jujube flower, which is applied in the field of jujube flower extract and its preparation, can solve the problem of no research reports on active chemical components, and achieve good antibacterial activity
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Embodiment 1
[0015] After crushing 5kg jujube flowers, add 15kg of ethanol water solution with a volume concentration of 90% for cold soaking, each cold soaking time is 48h, and cold soaking is 2 times in total; the cold soaking liquid is concentrated under reduced pressure at 35°C to obtain an extract About 760g; the extract was dispersed and extracted with ethyl acetate and water, and the ethyl acetate phase was concentrated under reduced pressure at 35°C to obtain 200g of jujube flower extract.
[0016] 200 g of jujube flower extract was mixed with 300 g of silica gel and loaded into a column for silica gel column chromatography with a column volume of 1.5 L. Use chloroform / methanol as the eluent for gradient elution, the elution gradients are 100:1, 60:1, 30:1, 5:1, 1:1, 0:1, and two column volumes are collected for each gradient Each effluent of gained is analyzed and detected by silica gel thin-layer plate, and the combined chloroform / methanol volume ratio is the eluent of 30:1 and 3...
Embodiment 2
[0020] The antibacterial activity of the jujube flower extract of embodiment 1
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