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Reagent for cleaning sequencing chip and its preparation method and application

A technology for sequencing chips and reagents, applied in detergent compositions, detergent compounding agents, chemical instruments and methods, etc., can solve the problems of poor cleaning effect, high toxicity of isopropanol, and irritating effect on mucous membranes. Sequencing quality, stable chemical safety, good cleaning effect

Active Publication Date: 2020-11-24
WUHAN BGI CLINICAL LAB CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, isopropanol is highly toxic, has obvious anesthetic effect, has a stimulating effect on the mucous membranes of the eyes and respiratory tract, and can damage the retina and optic nerve; it has been included in the list of three types of carcinogens by the International Agency for Research on Cancer of the World Health Organization
Moreover, the cleaning effect of ethanol and isopropanol is not good, and it is easy to decompose and volatilize, especially under high temperature storage conditions, it will decompose and cause the cleaning agent to fail

Method used

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  • Reagent for cleaning sequencing chip and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The reagents used for sequencing chip cleaning in this example include 10%-25% limonol by mass fraction, 5%-14% saponin extract, 8%-20% potassium hydroxide, 0.3%-2% EDTA, 1%-7% epoxy soybean oil, 0.1%-3% polyether modified silicone oil, 10%-20% ethanol and 23%-60% pure water; The amount of components used to prepare sequencing chip cleaning reagents with multiple formulations, and tested the surface volatilization time, light transmittance and intensity A of these sequencing chip cleaning reagents, as a comparison, at the same time comparatively tested using pure water and Illumina recommended Surface volatilization time, light transmittance, and intensity A performance of cleaning reagents, among which, Illumina recommends cleaning reagents that are equal volumes of ethanol and isopropanol mixed. In addition, some cleaning reagents were also set up using limonol, saponin extract, potassium hydroxide, EDTA, epoxidized soybean oil, polyether modified silicone oil, ethano...

Embodiment 2

[0050] In this example, the sequencing chip cleaning reagent of the formula 14 in Example 1 (hereinafter referred to as formula 14) was used for the test. At the same time, the same cleaning reagent recommended by Illumina as in Example 1 was used as a comparison to compare the sequencing chips after cleaning with different reagents. impact on subsequent sequencing quality. Specifically, in this example, three libraries were used for the test, and the three libraries were all phix control libraries, all of which were provided by the illumina kit product number: FC-110-3001. Each library was equally divided into two parts, one was sequenced with the sequencing chip cleaned with the sequencing chip cleaning reagent of Formula 14, and the other was sequenced with the sequencing chip cleaned with the cleaning reagent recommended by Illumina. The cleaning method of the sequencing chip is the same as that in Example 1. Except for the different reagents used for cleaning the sequenc...

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Abstract

A cleaning agent for a sequencing chip and a preparing method and application thereof are provided. The cleaning agent includes nerol, Chinese honeylocust extract liquid, potassium hydroxide, EDTA, epoxidized soybean oil, polyether modified silicone oil, ethanol and pure water. Components of the cleaning agent are designed aiming at oil stains and other pollutant sources of the sequencing chip, aplurality of plant extracts are adopted as active degreasing agents, the components in the cleaning agent cooperate with each other so that the sequencing chip can be cleaned effectively to remove surface oil stains and other impurities, no spot is left after cleaning, fogging is avoided and cleaning effects are good. The cleaning agent does not contain chloride or lower aromatic hydrocarbons, hasmain components which are plant extracts and is environmentally friendly, safe, stable and free of halogen, and therefore, the cleaning agent does not destroy the ozone layer. The cleaning agent specially used for the sequencing chip is provided.

Description

technical field [0001] The present application relates to the field of sequencing chip cleaning, in particular to a reagent for cleaning the sequencing chip and its preparation method and application. Background technique [0002] The purpose of cleaning the sequencing chip is to ensure that the sequencing chip is clean and pollution-free, thereby ensuring the quality of sequencing. At present, there is no special sequencing chip cleaning reagent, and there is a lack of corresponding research and reports. In practice, it is usually enough to use ethanol and isopropanol to clean the chip according to the chip manufacturer's suggestion. The role of ethanol and isopropanol is to dissolve and remove organic matter, accelerate volatilization, and achieve the effect of cleaning the sequencing chip. However, isopropanol is highly toxic, has obvious anesthetic effect, stimulates the mucous membrane of the eyes and respiratory tract, and can damage the retina and optic nerve; it ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11D1/66C11D3/04C11D3/20C11D3/33C11D3/37C11D3/60
CPCC11D1/662C11D3/044C11D3/201C11D3/2031C11D3/2096C11D3/33C11D3/3738
Inventor 刘晨黄金田志坚许振朋丁芬吴昊
Owner WUHAN BGI CLINICAL LAB CO LTD