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Preparation and application of bacillus megatherium BM22 and spore powder thereof

A technology of Bacillus megaterium and BM22, applied in the field of microorganisms, can solve the problems of easily polluting the environment and destroying the ecology, and achieve the effects of good storage, low cost and good control effect

Active Publication Date: 2018-11-30
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease is generally controlled by chemical methods, but since the long-term application of chemical pesticides is easy to pollute the environment and destroy the ecology, the comprehensive disease prevention and control measures have yet to be developed.

Method used

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  • Preparation and application of bacillus megatherium BM22 and spore powder thereof
  • Preparation and application of bacillus megatherium BM22 and spore powder thereof
  • Preparation and application of bacillus megatherium BM22 and spore powder thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and Identification of Bacillus megaterium BM22

[0029] 1.1 Isolation, purification and preservation of endophytic bacteria

[0030] Healthy leaves of Eucommia ulmoides were collected from the black spot occurrence area of ​​Eucommia ulmoides in Dayi, Sichuan. Rinse the collected samples with sterile water, weigh 1.0g of tissue, soak in 70% alcohol for 1-2min, then disinfect with 1-3% sodium hypochlorite solution for 3-5min, wash several times with sterile water, absorb Apply 100 μL of washing liquid for the last time to NA plate (3g beef extract, 10g peptone, 5g sodium chloride, 15-20g agar powder, 1000mL distilled water, pH 7.0, after mixing and dispensing, autoclave at 121°C for 30min), culture in the dark at 27°C 24h, used as a control for surface disinfection, to check whether the surface disinfection is thorough.

[0031] Cut the surface-sterilized bark tissue into pieces, put them into a sterile mortar, add sterilized quartz sand and 10mL of...

Embodiment 2

[0046] The preparation of embodiment 2 bacillus megaterium BM22 spore powder

[0047] The bacillus megaterium BM22 obtained according to embodiment 1 is made into spore powder preparation through seed culture and fermentation culture, specifically through the following methods:

[0048] The conventional method is to prepare beef extract peptone slant medium (beef extract 5g, peptone 10g, NaCl 3.5g, agar 18g, water 1000mL), inoculate Bacillus megaterium, and cultivate to make first-class seeds.

[0049] Nutritious succulent culture solution (beef extract 7g, peptone 10g, NaCl 5g, magnolia officinalis root bark soaking solution 1000mL) was autoclaved, and under oxygen control, bottled according to the proportion of 100mL liquid in a 300mL triangular flask, Inoculate Bacillus megaterium slant seeds in the state, inoculate 2 slant seeds per bottle, vibrate and cultivate, and make Bacillus megaterium liquid seeds. Wherein, 10 g of Photinia fragrans leaves are boiled in 1000 mL of ...

Embodiment 3

[0054] Example 3 Bacillus megaterium BM22 spore powder preparation spray method control test

[0055] According to the occurrence degree of erythema, it is divided into 4 occurrence areas (I: 5-10%; II: 11-30%; III: 31-60%; IV: >60%). In 4-5 months, the inoculum prepared in Example 2 adopts the concentration of spray method: 50 times of dilution, 100 times of dilution, 200 times of dilution, 400 times of dilution, 800 times of dilution, 1600 times of dilution, 2400 times of dilution to red leaves The leaves of Photinia were treated, sprayed evenly along the leaves, 100mL per plant, and sterile water was used as a control, repeated 3 times, and the incidence of Photinia fragrans was counted. 20 plants per treatment.

[0056] 15 days after treatment, conduct disease investigation and statistics according to the disease grading standards in Table 3, and calculate the control effect.

[0057] Table 3 Grading standard of Photinia erythema

[0058]

[0059] Incidence rate (%)=...

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Abstract

The invention discloses preparation and application of bacillus megatherium BM22 and spore powder thereof. The strain is preserved in CGMCC (China General Microbiological Culture Collection Center) onDecember 15, 2017, and has the preservation number being CGMCC No.15070. The bacillus megatherium BM22 is used as a strain to prepare the spore powder through seed culture, fermentation culture and kieselguhr adsorption. The bacillus megatherium BM22 provided by the invention has the special effects on photinia fraseri ulerythema; in addition, the stress resistance, good storage performance, hightemperature resistance, low temperature resistance and drying resistance are realized; the strain is applicable to commercials; the serious result of environment pollution due to chemical medicine can be avoided; the characteristics of sustainability and low cost are also realized.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a bacillus megaterium BM22 strain and its spore powder and its application in the prevention and treatment of Photinia erythema. Background technique [0002] Photinia (scientific name: Photinia × fraseri Dress) is a hybrid of Rosaceae and Photinia. It is an evergreen small tree or shrub. The height of the tree can reach 5 meters, and the height of the shrub can reach 2 meters. The crown is spherical, the leaves are leathery, oblong to obovate, lanceolate, the leaf tip is acuminate, the leaf base is wedge-shaped, the leaf margin has serrated glands, the flowers are many and dense, compound corymbs, white flowers , pome fruit yellow-red, blooming from May to July, and from September to October. It is mainly distributed in the subtropical and temperate regions of southeastern and eastern Asia and North America, and has been widely cultivated in many provinces of China...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/11
CPCA01N63/00C12N1/20C12N1/205C12R2001/11
Inventor 朱涵明月朱天辉
Owner SICHUAN AGRI UNIV
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